Overview
| Product Name |
Human COMP/Thrombospondin 5 ELISA Kit PicoKine™
See more COMP/Thrombospondin-5 products |
| Catalog# |
EK0913 |
| Storage & Handling |
Store at -20°C for one year. For short term storage and frequent use, store at 4°C for up to one month. Avoid repeated freeze-thaw cycles. |
| Description |
Human COMP/Thrombospondin 5 ELISA Kit PicoKine™ (96 Tests). Quantitate Human COMP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).. Sensitivity: 10pg/ml. |
| Cite This Product |
Human COMP/Thrombospondin 5 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0913)
|
| ELISA Validation |
ELISA Validation Information
|
Product Specs
| Reactivity/Species |
Human |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity |
<10 pg/ml |
| Assay Range |
156 pg/ml - 10,000 pg/ml |
| Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: Q21-A757 |
| Antibody Clonalities |
Capture antibody|Detection antibody
polyclonal antibody from goat|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For COMP (Source: Uniprot.org, NCBI)
| Uniprot Id | P49747 |
|---|
| NCBI Gene Id | 1311 |
|---|
| Species Of This Entry | Human |
|---|
| Gene Name | COMP |
|---|
| Protein Name | Cartilage oligomeric matrix protein |
|---|
| Superfamily | thrombospondin family |
|---|
| Alternative Names | COMP/Thrombospondin-5|cartilage oligomeric matrix protein (pseudoachondroplasia, epiphyseal dysplasia1, multiple); cartilage oligomeric matrix protein; cartilage oligomeric matrix protein(pseudoachondroplasia, epiphyseal dysplasia1, multiple); COMP; EDM1; EPD1; MED; MEDMGC131819; MGC149768; PSACH; pseudoachondroplasia (epiphyseal dysplasia 1, multiple); THBS5; Thrombospondin5; Thrombospondin-5; TSP5 |
|---|
| Molecular Weight | 82860 |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on COMP, check out the COMP Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for COMP: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the COMP infographic
Kit Components And Assay QC Data Details Of Human COMP/Thrombospondin 5 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0913-CAP |
Anti-Human COMP Pre-coated 96-well strip microplate | 1 |
| EK0913-ST | Human COMP Standard | 2 vials, 10 ng/tube |
| EK0913-DA | Human COMP Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0913 Human COMP/Thrombospondin 5 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.011 | 0.117 | 0.179 | 0.330 | 0.605 | 1.047 | 1.824 | 2.521 |
We measured random samples of Human COMP/Thrombospondin 5 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 233 | 1997 | 4935 | 255 | 1893 | 5413 |
| Standard deviation | 9.78 | 137.79 | 246.75 | 12.49 | 138.18 | 281.47 |
| CV (%) | 4.2% | 6.9% | 5% | 4.9% | 7.3% | 5.2% |
We measured the performance consistency of Human COMP/Thrombospondin 5 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 233 | 203 | 234 | 223 | 223 | 12.45 | 5.5% |
| Sample 2 | 1997 | 2142 | 1949 | 1828 | 1979 | 112.46 | 5.6% |
| Sample 3 | 4935 | 5185 | 4588 | 4986 | 4923 | 215.04 | 4.3% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
Here are featured tools and databases that you might find useful.
Total number of citations: 1
Plut D,Faganel Kotnik B,Preložnik Zupan I,Ključevšek D,Vidmar G,Snoj Ž,Salapura V. Detection and evaluation of haemophilic arthropathy: Which tools may be considered more reliable. Haemophilia.2020 Nov 8. doi: 10.1111/hae.14153.Epub ahead of print.PMID:33
Species: Human
EK0913 usage in article: APP:ELISA, SAMPLE:BLOOD, DILUTION:NA |
PubMed ID 33164312 |
Contact us with any questions at [email protected] or go to contact us
Questions and answers from customers related to EK0913 Human COMP/Thrombospondin 5 ELISA Kit PicoKine™
10 Related Questions
Question
I hope that you're doing well. Is product EK0913 predicted to work in Rat samples?
Verified Customer
Asked: 2019-12-05
Answer
We do not recommend the customer to use EK0913 on rat samples.
Boster Scientific Support
Answered: 2019-12-05
Question
For the kit EK0913 we have on file that the standard is produced using an NS0 expression system. A customer asks for the source of the promoter use on the expression vector for this recombinant protein.
Would you have this information available to share?
Verified Customer
Asked: 2018-07-02
Answer
We can not provide this information.
Boster Scientific Support
Answered: 2018-07-02
Question
Q: can you suggest the dilution ratio of serum samples for detection of COMP in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Asked: 2020-03-21
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the COMP ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-03-21
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Asked: 2019-09-30
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-09-30
Question
Q: which procedure should I follow in order to thaw whole blood sample for COMP ELISA after freezing?
Verified Customer
Asked: 2019-02-27
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test COMP for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-02-27
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring COMP using the EK0913 Human COMP Picokine® ELISA Kit?
V. Dayal
Asked: 2017-12-27
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2017-12-27
Question
Q: What is the optimal O.D. value for COMP ELISA kit? I performed your COMP ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain COMP even though the O.D. values are not very high?
Verified Customer
Asked: 2016-04-19
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this COMP ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of COMP that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2016-04-19
Question
Q: how do I analyze ELISA data? I measured COMP level in serum.
Verified Customer
Asked: 2014-10-07
Boster Scientific Support
Answered: 2014-10-07
Question
Has EK0913 been tested for bovine reactivity?
Answer
The Human COMP/Thrombospondin 5 ELISA Kit PicoKine™ (EK0913) hasn't been tested for bovine reactivity.
Boster Scientific Support
Answered: 2019-09-12
Question
Were both antibodies raised against the standard (aa 21-757), or were either of them raised against a shorter immunogen for EK0913?
Answer
For the Human COMP/Thrombospondin 5 ELISA Kit PicoKine™ (EK0913), both antibodies were raised against the standard (aa 21-757).
Boster Scientific Support
Answered: 2019-09-12
