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Product Info Summary
| SKU: | EK0913 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human COMP/Thrombospondin 5 ELISA Kit PicoKine®
SKU/Catalog Number
EK0913
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human COMP/Thrombospondin 5 ELISA Kit PicoKine® (96 Tests). Quantitate Human COMP in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human COMP/Thrombospondin 5 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0913)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Q21-A757
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0913 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0913 is reactive to COMP in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0913 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of COMP
Cartilage oligomeric matrix protein is a protein that in humans is encoded by the COMP gene. The sequences of rat and bovine COMP indicate that it is a member of the thrombospondin gene family. By Southern blot analysis of a somatic cell hybrid DNA panel and by isotopic in situ hybridization, human COMP gene was mapped to 19p13.1, and the murine COMP gene was mapped to the central region of mouse chromosome 8 by use of an interspecific backcross mapping panel. COMP is a marker of cartilage turnover.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0913-CAP | Anti-Human COMP Pre-coated 96-well strip microplate | 1 |
| EK0913-ST | Human COMP Standard | 2 vials, 10 ng/tube |
| EK0913-DA | Human COMP Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.02 | 0.066 | 0.12 | 0.231 | 0.462 | 0.918 | 1.816 | 2.572 |
Data Example Images
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Human COMP PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect COMP, Dilution ratio of 1:5000, concentration in serum is around 4000 ng/ml..
Some articles we found to cite concentrations of COMP in samples: 3389204 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human COMP/Thrombospondin 5 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 233 | 1997 | 4935 | 255 | 1893 | 5413 |
| Standard deviation | 9.78 | 137.79 | 246.75 | 12.49 | 138.18 | 281.47 |
| CV (%) | 4.2% | 6.9% | 5% | 4.9% | 7.3% | 5.2% |
Specific Publications For Human COMP/Thrombospondin 5 ELISA Kit PicoKine® (EK0913)
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Customer Reviews
0 Reviews For Human COMP/Thrombospondin 5 ELISA Kit PicoKine®
Customer Q&As
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9 Customer Q&As for Human COMP/Thrombospondin 5 ELISA Kit PicoKine®
Question
Q: can you suggest the dilution ratio of serum samples for detection of COMP in Human serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2020-03-21
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the COMP ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/elisa-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-03-21
Question
I hope that you're doing well. Is product EK0913 predicted to work in Rat samples?
Verified Customer
Verified customer
Asked: 2019-12-05
Answer
We do not recommend the customer to use EK0913 on rat samples.
Boster Scientific Support
Answered: 2019-12-05
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-09-30
Question
Has EK0913 been tested for bovine reactivity?
Verified customer
Asked: 2019-09-11
Answer
The Human COMP/Thrombospondin 5 ELISA Kit PicoKine™ (EK0913) hasn't been tested for bovine reactivity.
Boster Scientific Support
Answered: 2019-09-12
Question
Q: which procedure should I follow in order to thaw whole blood sample for COMP ELISA after freezing?
Verified Customer
Verified customer
Asked: 2019-02-27
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test COMP for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-02-27
Question
For the kit EK0913 we have on file that the standard is produced using an NS0 expression system. A customer asks for the source of the promoter use on the expression vector for this recombinant protein. Would you have this information available to share?
Verified Customer
Verified customer
Asked: 2018-07-02
Answer
We can not provide this information.
Boster Scientific Support
Answered: 2018-07-02
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring COMP using the EK0913 Human COMP Picokine® ELISA Kit?
V. Dayal
Verified customer
Asked: 2017-12-27
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2017-12-27
Question
Q: What is the optimal O.D. value for COMP ELISA kit? I performed your COMP ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain COMP even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2016-04-19
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this COMP ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of COMP that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2016-04-19
Question
Q: how do I analyze ELISA data? I measured COMP level in serum.
Verified Customer
Verified customer
Asked: 2014-10-07
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2014-10-07
