Human Cystatin B/CSTB ELISA Kit PicoKine™

Human Cystatin B ELISA Kit PicoKine™ (96 Tests). Quantitate Human CSTB in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1205
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Product Name

Human Cystatin B/CSTB ELISA Kit PicoKine™

See all Cystatin B/Stefin B products

SKU/Catalog Number

EK1205

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Cystatin B ELISA Kit PicoKine™ (96 Tests). Quantitate Human CSTB in cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Cystatin B/CSTB ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1205)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: M2-F98

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1205 is reactive to CSTB in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) , saliva, urine and human milk.

Application Guarantee

EK1205 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1205-CAP Anti-Human CSTB Pre-coated 96-well strip microplate 1
EK1205-ST Human CSTB Standard 2 vials, 10 ng/tube
EK1205-DA Human CSTB Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0250.1750.2960.5180.7711.6342.0122.283

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Cystatin B/Stefin B, Dilution ratio of 1:1, concentration in serum is around 1.7ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Cystatin B/CSTB ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)10852218241105321878
Standard deviation7.7732.88131.329.5740.96153.99
CV (%)7.2%7.7%7.2%8.7%7.7%8.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Cystatin B/Stefin B in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11081021011011032.912.8%
Sample 252246853745749634.136.8%
Sample 31824176719821870186078.944.2%
*number of samples for each test n=16.

Gene/Protein Information For CSTB (Source: Uniprot.Org, NCBI)

Uniprot ID

P04080

Gene ID

1476

Gene Name

CSTB

Full Name

Cystatin-B

Weight

11.14kDa

Superfamily

cystatin family

Alternative Names

CPI-B; CST6cystatin B (liver thiol proteinase inhibitor)10STFBcystatin-B; CSTB; cystatin B (stefin B); Cystatin B; EPM1; Liver thiol proteinase inhibitor; PME; Stefin B; stefin-B CSTB CPI-B, CST6, EPM1, EPM1A, PME, STFB, ULD cystatin B cystatin-B|cystatin B (stefin B)|epididymis secretory sperm binding protein|liver thiol proteinase inhibitor

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on CSTB, check out the CSTB Infographic

CSTB infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for CSTB: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Human Cystatin B/CSTB ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2020-05-25

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-05-25

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

L. Taylor

Verified customer

Asked: 2020-01-23

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-01-23

Question

Q: how can I thaw whole blood sample for Cystatin B ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-11-15

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Cystatin B for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-11-15

Question

Q: how to analyze ELISA data? I have obtained Cystatin B level in saliva.

H. Nelson

Verified customer

Asked: 2019-09-03

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-09-03

Question

Q: What is the optimal O.D. value for Cystatin B ELISA kit? I used your Cystatin B ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Cystatin B even though the O.D. values are not very high?

L. Brown

Verified customer

Asked: 2019-07-24

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Cystatin B ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Cystatin B that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2019-07-24

Question

Q: if the enzyme conjugated Cystatin B antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Cystatin B antigen?

Verified Customer

Verified customer

Asked: 2019-03-04

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-03-04

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis Cystatin B using the EK1205 Human Cystatin B Picokine® ELISA Kit?

P. Hall

Verified customer

Asked: 2018-09-08

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2018-09-08

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