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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human FCRL1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human FCRL1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FCRL1 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 20pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FCRL1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1930)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A17-S307
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1930 is reactive to FCRL1 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1930 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FCRL1/FcRH1
Fc receptor-like protein 1 is a protein that in humans is encoded by the FCRL1 gene. It is mapped to 1q23.1. This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains three extracellular C2-like immunoglobulin domains, a transmembrane domain and a cytoplasmic domain with two immunoreceptor-tyrosine activation motifs. This protein may play a role in the regulation of cancer cell growth. Alternative splicing results in multiple transcript variants.
|EK1930-CAP||Anti-Human FCRL1 Pre-coated 96-well strip microplate||1|
|EK1930-ST||Human FCRL1 Standard||2 vials, 10 ng/tube|
|EK1930-DA||Human FCRL1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human FCRL1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FCRL1/FcRH1, Dilution ratio of 1:1, concentration in serum is around 180pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FCRL1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of FCRL1/FcRH1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For FCRL1 (Source: Uniprot.Org, NCBI)
Fc receptor-like protein 1
BXMH1; CD307a antigen; CD307a; Fc receptor homolog 1; Fc receptor-like 1; FcRH1; FCRH1DKFZp667O1421; FCRL1; FcR-like protein 1; hIFGP1; IFGP family protein 1; IFGP1; IFGP1Fc receptor-like protein 1; Immune receptor translocation-associated protein 5; IRTA5; IRTA5immunoglobulin superfamily Fc receptor, gp42 FCRL1 CD307a, FCRH1, IFGP1, IRTA5 Fc receptor like 1 Fc receptor-like protein 1|IFGP family protein 1|fc receptor homolog 1|fcR-like protein 1|hIFGP1|immune receptor translocation-associated protein 5|immunoglobulin superfamily Fc receptor, gp42*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on FCRL1, check out the FCRL1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for FCRL1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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