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Product Info Summary
|Size:||96wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human FCRL2 ELISA Kit PicoKine®
96wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human FCRL2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human FCRL2 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human FCRL2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1976)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0, Immunogen sequence: L20-V401
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1976 is reactive to FCRL2 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK1976 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of FCRL2/FcRH2
Fc receptor-like protein 2 is a protein that in humans is encoded by the FCRL2 gene. It is mapped to 1q23.1. This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein has four extracellular C2-type immunoglobulin domains, a transmembrane domain and a cytoplasmic domain that contains one immunoreceptor-tyrosine activation motif and two immunoreceptor-tyrosine inhibitory motifs. This protein may be a prognostic marker for chronic lymphocytic leukemia. Alternatively spliced transcript variants have been described, but their biological validity has not been determined.
|EK1976-CAP||Anti-Human FCRL2 Pre-coated 96-well strip microplate||1|
|EK1976-ST||Human FCRL2 Standard||2 vials, 10 ng/tube|
|EK1976-DA||Human FCRL2 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human FCRL2 PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human FCRL2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of FCRL2/FcRH2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For FCRL2 (Source: Uniprot.Org, NCBI)
Fc receptor-like protein 2
CD307b antigen; CD307b; Fc receptor homolog 2; Fc receptor-like 2; FcRH2; FCRH2SPAP1B; FCRL2; FcR-like protein 2; IFGP family protein 4; IFGP4; Immunoglobulin receptor translocation-associated protein 4; immunoglobulin superfamily Fc receptor, gp42; IRTA4; IRTA4SPAP1C; SH2 domain containing phosphatase anchor protein 1; SH2 domain-containing phosphatase anchor protein 1Fc receptor-like protein 2; SPAP1; SPAP1A; SPAP1immune receptor translocation-associated protein 4 FCRL2 CD307b, FCRH2, IFGP4, IRTA4, SPAP1, SPAP1A, SPAP1B, SPAP1C Fc receptor like 2 Fc receptor-like protein 2|IFGP family protein 4|SH2 domain containing phosphatase anchor protein 1|fc receptor homolog 2|immune receptor translocation-associated protein 4|immunoglobulin receptor translocation-associated protein 4|immunoglobulin superfamily Fc receptor, gp42*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on FCRL2, check out the FCRL2 Infographic
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In this infographic you will see the following information for FCRL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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