Human Growth Hormone/GH1 ELISA Kit PicoKine™

Human Growth Hormone/GH1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human GH1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK0578
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human Growth Hormone/GH1 ELISA Kit PicoKine™

See all Growth Hormone products

SKU/Catalog Number

EK0578

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Growth Hormone/GH1 ELISA Kit PicoKine™ (96 Tests). Quantitate Human GH1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 2pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Growth Hormone/GH1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0578)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: F27-F217

Sensitivity

<2 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0578 is reactive to GH1 in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0578 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0578-CAP Anti-Human GH1 Pre-coated 96-well strip microplate 1
EK0578-ST Human GH1 Standard 2 vials, 10 ng/tube
EK0578-DA Human GH1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0420.1290.2290.3780.7091.2241.7472.498

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Growth Hormone/GH1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2919239428188423
Standard deviation2.211.1330.332.2911.8439.76
CV (%)7.6%5.8%7.7%8.2%6.3%9.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Growth Hormone in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 129293129290.862.9%
Sample 21921992001971973.081.5%
Sample 339442744344042619.424.5%
*number of samples for each test n=16.

Gene/Protein Information For GH1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P01241

Gene ID

2688

Gene Name

GH1

Full Name

Somatotropin

Weight

24.847kDa

Superfamily

somatotropin/prolactin family

Alternative Names

GH1; GHB5; GHIGHD1B; GHN; GH-N; GHNGrowth hormone; GH-Nsomatotropin; growth hormone 1Pituitary growth hormone; Growth Hormone; hGH-N; IGHD1A; IGHD2; Somatotropin GH1 GH, GH-N, GHB5, GHN, IGHD1A, IGHD1B, IGHD2, hGH-N growth hormone 1 somatotropin|growth hormone B5|pituitary growth hormone

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on GH1, check out the GH1 Infographic

GH1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for GH1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0578 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Alashti, M.,Shirvani, H.,Sabzevari Rad,R. et al.Effects of different small-sided games on blood lactate and GH/IGF-1 axis responses in young soccer players.Ger J Exerc Sport Res(2020).https://doi.org/10.1007/s1266 2-020-00691-6
Species: EK0578;APP:ELISA;SAMPLE:BLOOD;DILUTION:NA|EK0776;APP:ELISA;SAMPLE:BLOOD;DILUTION:NA

Inorganic nitrate mimics exercise-stimulated muscular fiber-type switching and myokine and %u03B3-aminobutyric acid release

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4 Customer Q&As for Human Growth Hormone/GH1 ELISA Kit PicoKine™

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

C. Lewis

Verified customer

Asked: 2020-07-16

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-07-16

Question

Q: Can Growth Hormone ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-06-20

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-06-20

Question

Q: is it okay to use citrate plasma as samples in Human Growth Hormone Picokine® ELISA Kit (Catalog # EK0578)?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Growth Hormone causing degradation of its protein structure. Growth Hormone may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Growth Hormone ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-09-17

Question

Q: What is the optimal O.D. value for Growth Hormone ELISA kit? I used your Growth Hormone ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Growth Hormone even though the O.D. values are not very high?

L. Martinez

Verified customer

Asked: 2019-01-04

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Growth Hormone ELISA kit to have sensitivity of 2pg/ml, that means the minimum amount of Growth Hormone that can be declared/interpreted as positive by the above standard is 2pg/ml.

Boster Scientific Support

Answered: 2019-01-04

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