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Product Info Summary
| SKU: | EK0430 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human IL-17/IL-17A ELISA Kit PicoKine®
SKU/Catalog Number
EK0430
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human IL-17/IL-17A ELISA Kit PicoKine® (96 Tests). Quantitate Human IL17A in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human IL-17/IL-17A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0430)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: I20-A155
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<1 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0430 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0430 is reactive to IL17A in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0430 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL17A
IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri (HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant human IL-17, consisting of 136 amino acids with the molecular mass of 16KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0430-CAP | Anti-Human IL17A Pre-coated 96-well strip microplate | 1 |
| EK0430-ST | Human IL17A Standard | 2 vials, 10 ng/tube |
| EK0430-DA | Human IL17A Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.056 | 0.11 | 0.156 | 0.244 | 0.457 | 0.868 | 1.677 | 2.955 |
Data Example Images
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Human IL-17 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IL17A, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..
Some articles we found to cite concentrations of IL17A in samples: 24076595 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human IL-17/IL-17A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 54 | 276 | 763 | 52 | 257 | 703 |
| Standard deviation | 3.88 | 19.59 | 34.33 | 4.52 | 18.5 | 37.96 |
| CV (%) | 7.2% | 7.1% | 4.5% | 8.7% | 7.2% | 5.4% |
Specific Publications For Human IL-17/IL-17A ELISA Kit PicoKine® (EK0430)
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Human IL-17/IL-17A ELISA Kit PicoKine®
Question
Is Human IL-17/IL-17A ELISA Kit PicoKine™ (EK0430) the same with Anti- IL17 ELISA?
Verified customer
Asked: 2021-01-26
Answer
The Anti- usually indicates antibodies rather than ELISA kits.
Boster Scientific Support
Answered: 2021-01-27
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
R. Martinez
Verified customer
Asked: 2020-03-05
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-03-05
Question
Q: if the enzyme conjugated IL-17 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-17 antigen?
Verified Customer
Verified customer
Asked: 2019-12-27
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-12-27
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
E. Huang
Verified customer
Asked: 2019-07-10
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-07-10
Question
What is the recommended dilution ratio of serum samples for the Human IL-17 ELISA Kit PicoKine?
Verified customer
Asked: 2019-06-17
Answer
The suggested dilution ratio for all the sample is 1:1.
Boster Scientific Support
Answered: 2019-06-17
Question
Q: is it okay to use citrate plasma as samples in Human IL-17 Picokine® ELISA Kit (Catalog # EK0430)?
S. Dhawan
Verified customer
Asked: 2019-05-17
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of IL-17 causing disruption of its protein structure. IL-17 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-17 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-05-17
Question
Is the standard in EK0430 a recombinant protein? If yes, what is the host species?
Verified customer
Asked: 2019-03-07
Answer
For the Human IL-17 ELISA Kit PicoKine™ (EK0430), the standard is a recombinant protein: Expression system for standard: E.coli
Boster Scientific Support
Answered: 2019-03-07
Question
Q: how can I thaw whole blood sample for IL-17 ELISA after freezing?
H. Dhawan
Verified customer
Asked: 2018-11-03
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-17 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-11-03
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
B. Patel
Verified customer
Asked: 2018-10-18
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2018-10-18
Question
Q: What is the optimal O.D. value for IL-17 ELISA kit? I performed your IL-17 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-17 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2016-04-03
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-17 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of IL-17 that can be declared/interpreted as positive by the above standard is 1pg/ml.
Boster Scientific Support
Answered: 2016-04-03
Question
Does this Human IL-17 ELISA Kit PicoKine detects forms A, F and A/F?
Verified Customer
Verified customer
Asked: 2015-12-09
Answer
The product Human IL-17 ELISA Kit PicoKine recognizes IL-17A but does not cross react with IL-17B, C, D, E and F. However at some degree of cross reactivity the kit recognize the IL-17A/F heterodimer based on sequence similarity.
Boster Scientific Support
Answered: 2015-12-09
Question
This is regarding IL 17 , Dr Preethi bought a kit on 11/4/2019.In this kit mentioned about the sample dilution .For this which dilution is recommended .Kindly check and reply me as soon as possible
Verified Customer
Verified customer
Asked: 2014-03-28
Answer
The suggested dilution ratio for all the sample is 1:1.
Boster Scientific Support
Answered: 2014-03-28
