Human IL-17/IL-17A ELISA Kit PicoKine®

IL-17/IL-17A ELISA kit for Human

Human IL-17/IL-17A ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL17A in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0430
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Customers Who Bought This Also Bought

Product Name

Human IL-17/IL-17A ELISA Kit PicoKine®

View all IL-17/IL-17A ELISA kits

SKU/Catalog Number

EK0430

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-17/IL-17A ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL17A in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human IL-17/IL-17A ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0430)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I20-A155

Sensitivity

<1 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0430 is reactive to IL17A in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0430 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL-17/IL-17A

IL-17 is an inflammatory cytokine produced primarily by a unique lineage of CD4 T cells that plays critical roles in the pathogenesis of multiple autoimmune diseases. Interleukin-17 is expressed by activated T cells and is 57% identical to the 17- to 26-kD secretory glycoprotein encoded by gene 13 of the herpesvirus saimiri (HVS-13). IL17 induces nuclear factor kappa-B and the expression of IL6, intercellular adhesion molecule-1, granulocyte macrophage colony-stimulating factor,and prostaglandin E2, as well as the maturation of CD34 positive hematopoietic precursors into neutrophils. Anti-IL17 antibodies significantly inhibited osteoclast formation induced by culture media of RA synovial tissues. The standard product used in this kit is recombinant human IL-17, consisting of 136 amino acids with the molecular mass of 16KDa.

Kit Components

Catalog Number Description Quantity
EK0430-CAP Anti-Human IL17A Pre-coated 96-well strip microplate 1
EK0430-ST Human IL17A Standard 2 vials, 10 ng/tube
EK0430-DA Human IL17A Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0450.0810.1630.2380.3830.6831.0451.963

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-17/IL-17A, Dilution ratio of 1:1.

Some articles we found to cite concentrations of IL-17/IL-17A in samples: 24076595 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-17/IL-17A ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5427676352257703
Standard deviation3.8819.5934.334.5218.537.96
CV (%)7.2%7.1%4.5%8.7%7.2%5.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-17/IL-17A in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 154546258573.315.8%
Sample 22762732832612737.942.9%
Sample 376376885478179136.674.6%
*number of samples for each test n=16.

Gene/Protein Information For IL17A (Source: Uniprot.Org, NCBI)

Uniprot ID

Q16552

Gene ID

3605

Gene Name

IL17A

Full Name

Interleukin-17A

Weight

17.504kDa

Superfamily

IL-17 family

Alternative Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A IL17A CTLA-8, CTLA8, IL-17, IL-17A, IL17 interleukin 17A interleukin-17A|cytotoxic T-lymphocyte-associated antigen 8|cytotoxic T-lymphocyte-associated protein 8|interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL17A, check out the IL17A Infographic

IL17A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL17A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0430 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Correlation between intestinal flora and serum inflammatory factors in patients with Crohns disease.

Imbalances of Th17 and Treg cells and their respective cytokines in COPD patients by disease stage

Elevated synovial fluid IL-33 and IL-6 levels and cartilage degeneration in stage III osteonecrosis of the femoral head

What is the impact of SOCS3, IL-35 and IL17 in immune pathogenesis of recurrent pregnancy loss?

Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of disease

Serum levels of TGFβ, IL-10, IL-17, and IL-23 cytokines in β-thalassemia major patients: the impact of silymarin therapy

The interleukin-17 G-197A polymorphism is associated with cyclosporine metabolism and transplant rejection in liver transplant recipients

The activation of IL-17 signaling pathway promotes pyroptosis in pneumonia-induced sepsis

Mesenchymal stromal cells ameliorate acute lung injury induced by LPS mainly through stanniocalcin-2 mediating macrophage polarization

Relationship between Th17-mediated immunity and airway inflammation in childhood neutrophilic asthma

Have you used Human IL-17/IL-17A ELISA Kit PicoKine®?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Human IL-17/IL-17A ELISA Kit PicoKine®

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

12 Customer Q&As for Human IL-17/IL-17A ELISA Kit PicoKine®

Question

Is Human IL-17/IL-17A ELISA Kit PicoKine™ (EK0430) the same with Anti- IL17 ELISA?

Verified customer

Asked: 2021-01-26

Answer

The Anti- usually indicates antibodies rather than ELISA kits.

Boster Scientific Support

Answered: 2021-01-27

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

R. Martinez

Verified customer

Asked: 2020-03-05

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-03-05

Question

Q: if the enzyme conjugated IL-17 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-17 antigen?

Verified Customer

Verified customer

Asked: 2019-12-27

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-12-27

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

E. Huang

Verified customer

Asked: 2019-07-10

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-07-10

Question

What is the recommended dilution ratio of serum samples for the Human IL-17 ELISA Kit PicoKine?

Verified customer

Asked: 2019-06-17

Answer

The suggested dilution ratio for all the sample is 1:1.

Boster Scientific Support

Answered: 2019-06-17

Question

Q: is it okay to use citrate plasma as samples in Human IL-17 Picokine® ELISA Kit (Catalog # EK0430)?

S. Dhawan

Verified customer

Asked: 2019-05-17

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of IL-17 causing disruption of its protein structure. IL-17 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-17 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-05-17

Question

Is the standard in EK0430 a recombinant protein? If yes, what is the host species?

Verified customer

Asked: 2019-03-07

Answer

For the Human IL-17 ELISA Kit PicoKine™ (EK0430), the standard is a recombinant protein: Expression system for standard: E.coli

Boster Scientific Support

Answered: 2019-03-07

Question

Q: how can I thaw whole blood sample for IL-17 ELISA after freezing?

H. Dhawan

Verified customer

Asked: 2018-11-03

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-17 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-11-03

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

B. Patel

Verified customer

Asked: 2018-10-18

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-10-18

Question

Q: What is the optimal O.D. value for IL-17 ELISA kit? I performed your IL-17 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-17 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2016-04-03

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-17 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of IL-17 that can be declared/interpreted as positive by the above standard is 1pg/ml.

Boster Scientific Support

Answered: 2016-04-03

Question

Does this Human IL-17 ELISA Kit PicoKine detects forms A, F and A/F?

Verified Customer

Verified customer

Asked: 2015-12-09

Answer

The product Human IL-17 ELISA Kit PicoKine recognizes IL-17A but does not cross react with IL-17B, C, D, E and F. However at some degree of cross reactivity the kit recognize the IL-17A/F heterodimer based on sequence similarity.

Boster Scientific Support

Answered: 2015-12-09

Question

This is regarding IL 17 , Dr Preethi bought a kit on 11/4/2019.In this kit mentioned about the sample dilution .For this which dilution is recommended .Kindly check and reply me as soon as possible

Verified Customer

Verified customer

Asked: 2014-03-28

Answer

The suggested dilution ratio for all the sample is 1:1.

Boster Scientific Support

Answered: 2014-03-28

$499

Ask a question

Get A Quote

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK0430
$499.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.