Human IL-17/IL-17A ELISA Kit PicoKine™

Human IL-17/IL-17A ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL17A in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml. Cited in 28 publication(s).

Product Info Summary

SKU: EK0430
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human IL-17/IL-17A ELISA Kit PicoKine™

See all IL-17/IL-17A products

SKU/Catalog Number

EK0430

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-17/IL-17A ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL17A in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IL-17/IL-17A ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0430)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: I20-A155

Sensitivity

<1 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0430 is reactive to IL17A in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0430 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0430-CAP Anti-Human IL17A Pre-coated 96-well strip microplate 1
EK0430-ST Human IL17A Standard 2 vials, 10 ng/tube
EK0430-DA Human IL17A Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0450.0810.1630.2380.3830.6831.0451.963

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-17/IL-17A, Dilution ratio of 1:1.

Some articles we found to cite concentrations of IL-17/IL-17A in samples: 24076595 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-17/IL-17A ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5427676352257703
Standard deviation3.8819.5934.334.5218.537.96
CV (%)7.2%7.1%4.5%8.7%7.2%5.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-17/IL-17A in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 154546258573.315.8%
Sample 22762732832612737.942.9%
Sample 376376885478179136.674.6%
*number of samples for each test n=16.

Gene/Protein Information For IL17A (Source: Uniprot.Org, NCBI)

Uniprot ID

Q16552

Gene ID

3605

Gene Name

IL17A

Full Name

Interleukin-17A

Weight

17.504kDa

Superfamily

IL-17 family

Alternative Names

CTLA8; CTLA-8; CTLA8cytotoxic T-lymphocyte-associated serine esterase 8; Cytotoxic T-lymphocyte-associated antigen 8; IL17; IL-17; IL17A; IL-17A; IL-17Acytotoxic T-lymphocyte-associated protein 8; IL-17CTLA-8; IL17interleukin-17A; interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8); interleukin 17A IL17A CTLA-8, CTLA8, IL-17, IL-17A, IL17 interleukin 17A interleukin-17A|cytotoxic T-lymphocyte-associated antigen 8|cytotoxic T-lymphocyte-associated protein 8|interleukin 17 (cytotoxic T-lymphocyte-associated serine esterase 8)

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL17A, check out the IL17A Infographic

IL17A infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL17A: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0430 has been cited in 28 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Interleukin-17 and Prostaglandin E2 Are Involved in Formation of an M2 Macrophage-Dominant Microenvironment in Lung Cancer
Species: Human

Yu D,Zhao Y,Wang H,Kong D,Jin W,Hu Y,Qin Y,Zhang B,Li X,Hao J,Li G,Wang H.IL-1β pre-stimulation enhances the therapeutic effects of endometrial regenerative cells on experimental colitis. Stem Cell Res Ther.2021 Jun 5;12(1):324.doi:10.1186/s13287-021-02392-9.PMID:34090510.
Species: Human,Mouse
EK0430 usage in article: APP:ELISA, SAMPLE:ERCS, DILUTION:NA

Charalampia Amerikanou,Eirini Dimitropoulou,Aristea Gioxari,Efstathia Papada,Anthi Tanaini,Charalambos Fotakis,Panagiotis Zoumpoulakis,Andriana C. Kaliora, Linking the IL-17A immune response with NMR-based faecal metabolic profile in IBD patients treated with Mastiha,Biomedicine & Pharmacotherapy,Volume 138,2021,111535,ISSN 0753-3322,https://doi. org/10.1016/j.biopha.2021.111535.
Species: Human
EK0430 usage in article: APP:ELISA, SAMPLE:STOOL, DILUTION:NA

Hussien HA,Habieb MS,Hamdan AM.Evaluation of Serum Total Immunoglobulin E, Interleukin-17 and Pentraxin-3 as Biomarkers for Chronic Rhinosinusitis With Nasal Polyposis.Am J Rhinol Allergy.2020 Dec 23:1945892420983787.doi:10.1177/1945892420983787.Epub ahea
Species: Human
EK0430 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Gioxari A,Amerikanou C,Papada E,Zioga E,Georgoulis AD,Bamias G,Kaliora AC.Serum Vitamins D, B9 and B12 in Greek Patients with Inflammatory Bowel Diseases. Nutrients. 2020 Dec 4;12(12):E3734.doi:10.3390 /nu12123734.PMID:33291539.
Species: Human
EK0430 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

What is the impact of SOCS3, IL-35 and IL17 in immune pathogenesis of recurrent pregnancy loss?

Effect of high dose vitamin D intake on interleukin-17 levels in multiple sclerosis: a randomized, double-blind, placebo-controlled clinical trial

Serum levels of TGFβ, IL-10, IL-17, and IL-23 cytokines in β-thalassemia major patients: the impact of silymarin therapy

Plasma IL-17, IL-35, interferon-γ, SOCS3 and TGF-β levels in pregnant women with preeclampsia, and their relation with severity of disease

Chronic intermittent hypoxia disturbs insulin secretion and causes pancreatic injury via the MAPK signaling pathway

Mutations in JAK2 and Calreticulin genes are associated with specific alterations of the immune system in myelofibrosis

Peripheral blood mononuclear cells of patients with latent autoimmune diabetes secrete higher levels of pro- & anti-inflammatory cytokines compared to those with type-1 diabetes mellitus following in vitro stimulation with ?-cell autoantigens

The inflammatory cytokine interleukin-23 is elevated in lung cancer, particularly small cell type

The role of soluble tumor necrosis factor like weak inducer of?apoptosis?and interleukin-17A in the etiopathogenesis of celiac?disease: A cross-sectional study

Changes in interleukin-17 and transforming growth factor beta 1 levels in serum and bronchoalveolar lavage fluid and their clinical significance among children with asthma

Min M, Yan BX, Wang P, Landeck L, Chen JQ, Li W, Cai SQ, Zheng M, Man XY. PLoS One. 2017 Dec 22;12(12):e0190051. doi: 10.1371/journal.pone.0190051. eCollection 2017. Rottlerin as a therapeutic approach?in psoriasis: Evidence from in vitro and in v...

Chai H, Yang L, Gao L, Guo Y, Li H, Fan X, Wu B, Xue S, Cai Y, Jiang X, Qin B, Zhang S, Ke Y. Transplantation. 2014 Oct 15;98(7):729-37. Doi: 10.1097/Tp.0000000000000319. Decreased Percentages Of Regulatory T Cells Are Necessary To Activate Th1-Th...

Li Xn, Pan X, Qiu D. Int J Clin Exp Med. 2014 Dec 15;7(12):5324-9. Ecollection 2014. Imbalances Of Th17 And Treg Cells And Their Respective Cytokines In Copd Patients By Disease Stage.

Jiang K, Chen Hb, Wang Y, Lin Jh, Hu Y, Fang Yr. Zhongguo Dang Dai Er Ke Za Zhi. 2013 Aug;15(8):604-8. [Changes In Il-17 And Tgf-??1 Levels In Serum And Bronchoalveolar Lavage Fluid And Their Clinical Significance Among Children With Asthma].

Liu L, Ge D, Ma L, Mei J, Liu S, Zhang Q, Ren F, Liao H, Pu Q, Wang T, You Z. J Thorac Oncol. 2012 Jul;7(7):1091-100. Doi: 10.1097/Jto.0B013E3182542752. Interleukin-17 And Prostaglandin E2 Are Involved In Formation Of An M2 Macrophage-Dominant Mic...

Lu R, Zeng X, Han Q, Lin M, Long L, Dan H, Zhou G, Chen Q. Mediators Inflamm. 2014;2014:701094. Doi: 10.1155/2014/701094. Epub 2014 Jul 8. Overexpression And Selectively Regulatory Roles Of Il-23/Il-17 Axis In The Lesions Of Oral Lichen Planus.

Yang B, Zhou X, Lan C. Bmc Gastroenterol. 2015 Apr 1;15:43. Doi: 10.1186/S12876-015-0272-8. Changes Of Cytokine Levels In A Mouse Model Of Post-Infectious Irritable Bowel Syndrome.

Jin Y, Wan Y, Chen G, Chen L, Zhang Mq, Deng L, Zhang Jc, Xiong Xz, Xin Jb. Plos One. 2014 Oct 16;9(10):E111044. Doi: 10.1371/Journal.Pone.0111044. Ecollection 2014. Treg/Il-17 Ratio And Treg Differentiation In Patients With Copd.

Sereshki N, Gharagozloo M, Ostadi V, Ghahiri A, Roghaei Ma, Mehrabian F, Andalib Aa, Hassanzadeh A, Hosseini H, Rezaei A. Int J Fertil Steril. 2014 Apr;8(1):59-66. Epub 2014 Mar 9. Variations In T-Helper 17 And Regulatory T Cells During The Menstr...

Xu R, Tao A, Zhang S, Zhang M. Acta Biochim Biophys Sin (Shanghai). 2013 Sep;45(9):726-33. Doi: 10.1093/Abbs/Gmt065. Epub 2013 Jun 19. Neutralization Of Interleukin-17 Attenuates High Fat Diet-Induced Non-Alcoholic Fatty Liver Disease In Mice.

Wang Sb, Deng Yq, Ren J, Xiao Bk, Chen Z, Tao Zz. Scand J Immunol. 2013 Dec;78(6):507-15. Doi: 10.1111/Sji.12118. Lactoferrin Administration Into The Nostril Alleviates Murine Allergic Rhinitis And Its Mechanisms.

Ou J, Shi W, Xu Y, Tao Z. Ann Allergy Asthma Immunol. 2014 Dec;113(6):658-665.E1. Doi: 10.1016/J.Anai.2014.08.015. Epub 2014 Sep 17. Intranasal Immunization With Dna Vaccine Coexpressing Der P 1 And Ubiquitin In An Allergic Rhinitis Mouse Model.

Ozkan Zs, Deveci D, Kumbak B, Simsek M, Ilhan F, Sekercioglu S, Sapmaz E. J Reprod Immunol. 2014 Jun;103:53-8. Doi: 10.1016/J.Jri.2013.11.002. Epub 2013 Dec 8. What Is The Impact Of Th1/Th2 Ratio, Socs3, Il17, And Il35 Levels In Unexplained Infert...

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12 Customer Q&As for Human IL-17/IL-17A ELISA Kit PicoKine™

Question

Is Human IL-17/IL-17A ELISA Kit PicoKine™ (EK0430) the same with Anti- IL17 ELISA?

Verified customer

Asked: 2021-01-26

Answer

The Anti- usually indicates antibodies rather than ELISA kits.

Boster Scientific Support

Answered: 2021-01-27

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

R. Martinez

Verified customer

Asked: 2020-03-05

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-03-05

Question

Q: if the enzyme conjugated IL-17 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-17 antigen?

Verified Customer

Verified customer

Asked: 2019-12-27

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-12-27

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

E. Huang

Verified customer

Asked: 2019-07-10

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can change immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-07-10

Question

What is the recommended dilution ratio of serum samples for the Human IL-17 ELISA Kit PicoKine?

Verified customer

Asked: 2019-06-17

Answer

The suggested dilution ratio for all the sample is 1:1.

Boster Scientific Support

Answered: 2019-06-17

Question

Q: is it okay to use citrate plasma as samples in Human IL-17 Picokine® ELISA Kit (Catalog # EK0430)?

S. Dhawan

Verified customer

Asked: 2019-05-17

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of IL-17 causing disruption of its protein structure. IL-17 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the IL-17 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-05-17

Question

Is the standard a recombinant protein for EK0430? If yes, what is the host species?

Verified customer

Asked: 2019-03-07

Answer

For the Human IL-17 ELISA Kit PicoKine™ (EK0430), the standard is a recombinant protein: Expression system for standard: E.coli

Boster Scientific Support

Answered: 2019-03-07

Question

Q: how can I thaw whole blood sample for IL-17 ELISA after freezing?

H. Dhawan

Verified customer

Asked: 2018-11-03

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-17 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-11-03

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

B. Patel

Verified customer

Asked: 2018-10-18

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-10-18

Question

Q: What is the optimal O.D. value for IL-17 ELISA kit? I performed your IL-17 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IL-17 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2016-04-03

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-17 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of IL-17 that can be declared/interpreted as positive by the above standard is 1pg/ml.

Boster Scientific Support

Answered: 2016-04-03

Question

Does this Human IL-17 ELISA Kit PicoKine detects forms A, F and A/F?

Verified Customer

Verified customer

Asked: 2015-12-09

Answer

The product Human IL-17 ELISA Kit PicoKine recognizes IL-17A but does not cross react with IL-17B, C, D, E and F. However at some degree of cross reactivity the kit recognize the IL-17A/F heterodimer based on sequence similarity.

Boster Scientific Support

Answered: 2015-12-09

Question

This is regarding IL 17 , Dr Preethi bought a kit on 11/4/2019.In this kit mentioned about the sample dilution .For this which dilution is recommended .Kindly check and reply me as soon as possible

Verified Customer

Verified customer

Asked: 2014-03-28

Answer

The suggested dilution ratio for all the sample is 1:1.

Boster Scientific Support

Answered: 2014-03-28

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