Human IL-17C ELISA Kit PicoKine®

IL-17C ELISA kit for Human

Human IL-17C ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL17C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK0789
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human IL-17C ELISA Kit PicoKine®

View all IL-17C ELISA kits

SKU/Catalog Number

EK0789

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-17C ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL17C in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human IL-17C ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0789)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: H19-V197

Sensitivity

<10 pg/ml

Assay Range

125 pg/ml - 8,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK0789 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0789 is reactive to IL17C in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0789 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL-17C

IL17C, also known as CX2, is a protein that in humans is encoded by the IL17C gene. IL17C is mapped to 16q24.3. The protein encoded by this gene is a T cell-derived cytokine that shares the sequence similarity with IL17. This cytokine was reported to stimulate the release of tumor necrosis factor alpha and interleukin 1 beta from a monocytic cell line. The expression of this cytokine was found to be restricted to activated T cells. IL17C is an essential autocrine cytokine regulating innate epithelial immune responses. It also plays an important role in the pathogenesis of inflammatory arthritis.

Kit Components

Catalog Number Description Quantity
EK0789-CAP Anti-Human IL17C Pre-coated 96-well strip microplate 1
EK0789-ST Human IL17C Standard 2 vials, 10 ng/tube
EK0789-DA Human IL17C Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)01252505001000200040008000
O.D.0.1120.2090.2700.3960.6530.9701.4221.821

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-17C, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 125 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-17C ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)1881000586117210826289
Standard deviation12.465304.7711.5288.72377.34
CV (%)6.6%6.5%5.2%6.7%8.2%6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-17C in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 118815815717216812.597.4%
Sample 21000103095594198135.483.6%
Sample 358616256601456555946219.443.6%
*number of samples for each test n=16.

Gene/Protein Information For IL17C (Source: Uniprot.Org, NCBI)

Gene Name

IL17C

Full Name

Interleukin-17C

Weight

21.765kDa

Superfamily

IL-17 family

Alternative Names

CX2; Cytokine CX2; IL17C; IL-17C; IL-17CMGC126884; interleukin 17C; interleukin-17C; MGC138401 IL17C CX2, IL-17C interleukin 17C interleukin-17C|cytokine CX2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL17C, check out the IL17C Infographic

IL17C infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL17C: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

EK0789 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

‘Psoriasis 1’ reduces psoriasis‑like skin inflammation by inhibiting the VDR‑mediated nuclear NF‑κB and STAT signaling pathways

Psoriasis 1'reduces psoriasis%u2011like skin inflammation by inhibiting the VDR%u2011mediated nuclear NF%u2011%u03BAB and STAT signaling pathways

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7 Customer Q&As for Human IL-17C ELISA Kit PicoKine®

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-08-04

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2020-08-04

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of IL-17C in Human serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-04-24

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-17C ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-04-24

Question

Q: which procedure should I follow in order to thaw whole blood sample for IL-17C ELISA after freezing?

Verified Customer

Verified customer

Asked: 2019-06-20

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-17C for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-06-20

Question

Can EK0789 and EK0793 be used with skin biopsies? If yes, is there a need to modify the protocol?

Verified customer

Asked: 2019-03-27

Answer

Our lab has not worked on skin biopsies using the Human IL-17C ELISA Kit PicoKine™ (EK0789) and Human IL-17E/IL-25 ELISA Kit PicoKine™ (EK0793). We suggest you to run a pilot test using the kits. The following is the suggested sample preparation protocol; 1. Mince skin biopsies and then rinse skin biopsies in ice-cold PBS. 2. Place minced skin biopsies in tissue homogenizer. 3. Add ice-cold PBS. For a 0.5g piece of tissue, add 5ml of ice-cold PBS (Volumes of PBS must be determined in relation to the amount of skin biopsies present.) 5. Homogenize for several minutes at high speed. 6. Sonicate until no skin biopsies chunks remain. 7. Centrifuge at ~10000 x g for 10 minutes. The centrifugation force and time can be varied depending on the sample type. 8. Aspirate supernatant. 9. Aliquot supernatant and store at -20°C. There is no need to adjust the main protocol.

Boster Scientific Support

Answered: 2019-03-28

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-01-15

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-01-15

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

G. Babu

Verified customer

Asked: 2018-11-11

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-11-11

Question

Q: if the enzyme conjugated IL-17C antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-17C antigen?

Verified Customer

Verified customer

Asked: 2017-02-25

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-02-25

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