Human IL-18BP ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human IL-18BP. 96wells/kit, with removable strips.
Human IL-18BP ELISA Kit PicoKine™ Info At A Glance
|Size:||96wells/kit, with removable strips.|
|Sample Type:||cell culture supernates, serum, plasma(heparin, EDTA) and urine.|
|Sample Volume:||100μl per well|
|Product Name||Human IL-18BP ELISA Kit PicoKine™
See all IL18BP primary antibodies, ELISA kits and proteins
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Detect Human Il18 Binding Protein/IL18BP with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Il18 Binding Protein/IL18BP tissue specificity: Strongly expressed in heart, lung, placenta and spleen. .|
|Cite This Product||Human IL-18BP ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1282)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernates, serum, plasma(heparin, EDTA) and urine.|
|Immunogen/Standard||Expression system for standard: NSO; Immunogen sequence: T31-G194|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96wells/kit, with removable strips.|
Gene/Protein Basic Information For IL18BP (Source: Uniprot.org, NCBI)
|NCBI Gene Id||10068|
|Species Of This Entry||Human|
|Protein Name||Interleukin-18-binding protein|
|Alternative Names||IL18BP|IL18 BPa; IL-18 BPa; IL18BP; IL-18BP; IL18BPa; interleukin 18 binding protein; interleukin-18-binding protein; MC51L-53L-54L homolog gene product; tadekinig-alfa|
|Post Tranlational Modifications||Cleavage; Phosphorylation|
|Gene Location||11q13.4, on Chromasome 11, gene sequence: NC_000011.10 (71997944..72002919)|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For IL18BP (Source: Uniprot.org, NCBI)
|Protein Function||Isoform A binds to IL-18 and inhibits its activity. Functions as an inhibitor of the early TH1 cytokine response.|
|Tissue Specificity||Strongly expressed in heart, lung, placenta and spleen.|
|Research Areas||Cytokine Research; Immunology; Innate Immunity|
|Related Diseases||Inflammation; Infertility; Rheumatoid Arthritis; Myocardial Ischemia; Coronary Heart Disease; Neoplasms, Radiation-induced; Infection Due To Brucella Ovis; Endometrial Polyp; Sheep Diseases; Malignant Neoplasm Of Lung; Myocardial Infarction; Acute Hepatitis; Malignant Neoplasms; Neoplasms; Nervousness; Thrombocytopenic Purpura; Arthritis, Psoriatic; Purpura, Thrombocytopenic, Idiopathic; Mammary Neoplasms, Experimental|
|Related Pathways||Pathogenesis; Immune Response; Mrna Splicing; Phagocytosis; Virulence; Secretion|
|Background||Interleukin-18-binding protein, also known as IL18BPa, is a protein that in humans is encoded by the IL18BP gene. It is mapped to 11q13.4. The protein encoded by this gene is an inhibitor of the proinflammatory cytokine IL18. This protein binds to IL18, prevents the binding of IL18 to its receptor, and thus inhibits IL18-induced IFN-gamma production. This protein is constitutively expressed and secreted in mononuclear cells. The expression of this protein can be enhanced by IFN-gamma. It has been found that IL18BP expression in intestinal tissue is increased in endothelial cells as well as cells of the submucosa and overlying lymphoid aggregates in Crohn disease.|
|Scientific References||PMID: 10023777 by Novick D., et al. Interleukin-18 binding protein: a novel modulator of the Th1 cytokine response.|
PMID: 10094485 by Aizawa Y., et al. Cloning and expression of interleukin-18 binding protein.
Kit Components And Assay QC Data Details Of Human IL-18BP ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK1282-CAP||96-well plate precoated with anti-Human IL18BP antibody||1|
|EK1282-ST||lyophilized recombinant Human IL18BP standard||10ng/tube|
|EK1282-DA||biotinylated anti-Human IL18BP antibody||130ul|
|AR1106-1||sample diluent buffer||30ml|
|AR1106-2||antibody diluent buffer||12ml|
|AR1106-3||ABC diluent buffer||12ml|
|AR1104||TMB color developing agent||10ml|
|AR1105||TMB stop solution||10ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1282 Human IL-18BP ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Human IL-18BP ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Human IL-18BP ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot1 (pg/ml)||Lot2 (pg/ml)||Lot3 (pg/ml)||Lot4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
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