Human IL-2/Interleukin-2 ELISA Kit PicoKine™

Human IL-2/Interleukin-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL2 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Sensitivity: 1pg/ml. Cited in 22 publication(s). Independently reviewed in 1 review(s).

Product Info Summary

SKU: EK0397
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).

Product Name

Human IL-2/Interleukin-2 ELISA Kit PicoKine™

See all IL-2 products

SKU/Catalog Number

EK0397

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human IL-2/Interleukin-2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human IL2 in cell culture supernatants, serum and plasma ( heparin, EDTA, citrate). Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human IL-2/Interleukin-2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0397)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: A21-T153

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0397 is reactive to IL2 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma ( heparin, EDTA, citrate).

Application Guarantee

EK0397 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0397-CAP Anti-Human IL2 Pre-coated 96-well strip microplate 1
EK0397-ST Human IL2 Standard 2 vials, 10 ng/tube
EK0397-DA Human IL2 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0300.0650.1230.2120.3360.6091.0311.798

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-2, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of IL-2 in samples: 22040441 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human IL-2/Interleukin-2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3115848428169474
Standard deviation1.278.84301.1410.9838.39
CV (%)4.1%5.6%6.2%4.1%6.5%8.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 131292830291.113.8%
Sample 21581601511631584.412.7%
Sample 34844694884804807.081.4%
*number of samples for each test n=16.

Gene/Protein Information For IL2 (Source: Uniprot.Org, NCBI)

Uniprot ID

P60568

Gene ID

3558

Gene Name

IL2

Full Name

Interleukin-2

Weight

17.628kDa

Superfamily

IL-2 family

Alternative Names

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; T cell growth factor; T-cell growth factor; TCGF IL2 IL-2, TCGF, lymphokine interleukin 2 interleukin-2|T cell growth factor|aldesleukin|involved in regulation of T-cell clonal expansion

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IL2, check out the IL2 Infographic

IL2 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IL2: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0397 has been cited in 22 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

USP2a positively regulates TCR-induced NF-κB activation by bridging MALT1-TRAF6
Species: Human

Yonglan Wang,Kun Cao,"KDM1A Promotes Immunosuppression in Hepatocellular Carcinoma by Regulating PD-L1 through Demethylating MEF2D",Journal of Immunology Research,vol.2021,Article ID 9965099,19 pages,2021.https://doi.org/10.1155/2021/9965099
Species: Human
EK0397 usage in article: APP:ELISA, SAMPLE:H22 CELL, DILUTION:NA

il-8 regulates the stemness properties of cancer stem cells in the small-cell lung cancer cell line h446

Characteristics of CD4+CD25+Foxp3+?regulatory T cells in patients with multiple organ dysfunction syndrome

Immunomodulatory effects of total intravenous and balanced inhalation anesthesia in patients with bladder cancer undergoing elective radical Cystectomy: preliminary results

Han XR, Wen X, Li YY, Fan SH, Zhang ZF, Li H, Sun XF, Geng GQ, Sun S, Huang SQ, Wu DM, Lu J, Zheng YL. Biosci Rep. 2017 Oct 24;37(5). pii: BSR20170915. doi: 10.1042/BSR20170915. Print 2017 Oct 31. Effect of different anesthetic methods on cellular...

Tong Gq, Zhang Zh, Zhao Y, Liu Jj, Han Jb. Arch Environ Contam Toxicol. 2015 Jan;68(1):31-7. Doi: 10.1007/S00244-014-0077-8. Epub 2014 Sep 7. Traffic-Related Pm2.5 Induces Cytosolic [Ca?????] Increase Regulated By Orai1, Alters The Can-Nfat Signal...

Cheng K, Wu Z, Gao B, Xu J. Cell Biochem Biophys. 2014 Nov;70(2):1305-9. Doi: 10.1007/S12013-014-0055-9. Analysis Of Influence Of Baicalin Joint Resveratrol Retention Enema On The Tnf-??, Siga, Il-2, Ifn-?? Of Rats With Respiratory Syncytial Virus...

Liang Y, Liu Hh, Chen Yj, Sun H. J Med Food. 2014 Apr;17(4):439-46. Doi: 10.1089/Jmf.2013.2846. Epub 2014 Mar 4. Antitumor Activity Of The Protein And Small Molecule Component Fractions From Agrocybe Aegerita Through Enhancement Of Cytokine Produc...

Hu W,, Jing P, Wang L, Zhang Y, Yong J, Wang Y. Bmc Complement Altern Med. 2015 Apr 15;15:119. Doi: 10.1186/S12906-015-0642-3. The Positive Effects Of Ginsenoside Rg1 Upon The Hematopoietic Microenvironment In A D-Galactose-Induced Aged Rat Model.

Chen F, Ni Y, Ye Y, Sun H, Li X, Xu S. Int Immunopharmacol. 2010 Oct;10(10):1153-60. Doi: 10.1016/J.Intimp.2010.06.021. Epub 2010 Jul 4. Comparison Of Immunosuppressive Activity Of Stephanoside E And Its Aglycone From Stephanotis Mucronata In Vitro.

Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????

Zhu Hc, Qiu T, Liu Xh, Dong Wc, Weng Xd, Hu Ch, Kuang Yl, Gao Rh, Dan C, Tao T. Cell Immunol. 2012;274(1-2):12-8. Doi: 10.1016/J.Cellimm.2012.02.012. Epub 2012 Mar 14. Tolerogenic Dendritic Cells Generated By Relb Silencing Using Shrna Prevent Acu...

Guida F, Luongo L, Aviello G, Palazzo E, De Chiaro M, Gatta L, Boccella S, Marabese I, Zjawiony Jk, Capasso R, Izzo Aa, De Novellis V, Maione S. Mol Pain. 2012 Aug 23;8:60. Doi: 10.1186/1744-8069-8-60. Salvinorin A Reduces Mechanical Allodynia And...

Liu Sh, Shen Xh, Wei Xf, Mao Xh, Huang T. Immunopharmacol Immunotoxicol. 2011 Mar;33(1):100-6. Doi: 10.3109/08923973.2010.483520. Epub 2010 May 20. Immunomodulatory Activity Of Butanol Extract From Solanum Lyratum In Tumor-Bearing Mice.

Eker Ss, Yavasci Eo, Cangur S, Kirli S, Sarandol E. Acta Neuropsychiatr. 2014 Oct;26(5):291-7. Doi: 10.1017/Neu.2014.13. Can Bdnf And Il-2 Be Indicators For The Diagnosis In Schizophrenic Patients With Depressive Symptoms?

Li Y, He X, Wang S, Shu Hb, Liu Y. Protein Cell. 2013 Jan;4(1):62-70. Doi: 10.1007/S13238-012-2120-8. Epub 2012 Dec 20. Usp2A Positively Regulates Tcr-Induced Nf-??b Activation By Bridging Malt1-Traf6.

Li C, Liang J, Ma Z, Hu Y, Yan Z, Li Q, Fang Z, Wang H, Zhang G, Xie L, Zhang R. Fish Shellfish Immunol. 2010 Feb;28(2):253-60. Doi: 10.1016/J.Fsi.2009.11.001. Epub 2009 Nov 5. Calcineurin Mediates The Immune Response Of Hemocytes Through Nf-Kappa...

Zhong G, Cheng X, Long H, He L, Qi W, Xiang T, Zhao Z, Zhu B. J Transl Med. 2013 Mar 21;11:71. Doi: 10.1186/1479-5876-11-71. Dynamically Expressed Microrna-15B Modulates The Activities Of Cd8+ T Lymphocytes In Mice With Lewis Lung Carcinoma.

Sofian M, Aghakhani A, Farazi Aa, Banifazl M, Eslamifar A, Rashidi N, Khadem Sadegh A, Ramezani A. Hepat Mon. 2012 Dec;12(12):E6156. Doi: 10.5812/Hepatmon.6156. Epub 2012 Dec 29. Serum Profile Of T Helper 1 And T Helper 2 Cytokines In Hepatitis C ...

Xu Y, Feng L, Wang S, Zhu Q, Lin J, Lou C, Xiang P, He B, Zheng Z, Tang D, Zuo G. J Cell Biochem. 2011 Oct;112(10):2953-65. Doi: 10.1002/Jcb.23212. Phytoestrogen Calycosin-7-O-??-D-Glucopyranoside Ameliorates Advanced Glycation End Products-Induce...

Liu W, Shan Lp, Dong Xs, Liu Xw, Ma T, Liu Z. World J Gastroenterol. 2013 Jan 28;19(4):492-502. Doi: 10.3748/Wjg.V19.I4.492. Combined Early Fluid Resuscitation And Hydrogen Inhalation Attenuates Lung And Intestine Injury.

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1 Reviews For Human IL-2/Interleukin-2 ELISA Kit PicoKine™

0

"The standards and ELISA kit worked great."

Excellent

Source: Biocompare.com

"The standards and ELISA kit worked great."

--Jason Somarelli, DUKE UNIVERSITY, MEDICINE, RESEARCH ASSOCIATE

Applications Detection of secreted IL-2 in media of Jurkat T cells
Sample Jurkat T cell media (RPMI, 10% FBS, 1% pen/strep)
Detection --

"I was using this kit to detect IL-2 secretion from Jurkat T-cells upon activation with PMA and PHA. I followed the protocol exactly. I had extremely low levels of IL-2 in my experiment, which were just above background. In this case, it was essential for me to use the standards and blanks to ensure that my samples were above background. All components come with the kit, unlike some other kits from other companies in which only some of the necessary components are provided. This is a great kit that is a bit expensive, but worth the cost for an easy-to-use and sensitive assay."

Verified customer

Submitted

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9 Customer Q&As for Human IL-2/Interleukin-2 ELISA Kit PicoKine™

Question

What would be the effects of incubating at room temperature versus 37°C for EK0397?

Verified customer

Asked: 2021-01-13

Answer

For the Human IL-2 ELISA Kit PicoKine™ (EK0397), it takes longer to incubate at room temperature than at 37°C. https://www.bosterbio.com/datasheet?sku=EK0397 Please see the following detailed information. 4. Cover with the plate sealer provided and incubate for 120 minutes at RT (or 90 min. at 37 °C) 7. Cover with plate sealer and incubate for 90 minutes at RT (or 60 minutes at 37°C). 9. Add 100 µl of the prepared 1x Avidin-Biotin-Peroxidase Complex into each well. Cover with the plate sealer provided and incubate for 40 minutes at RT (or 30 minutes at 37°C). 11. Add 90 µl of Color Developing Reagent to each well. Cover with the plate sealer provided and incubate in the dark for 30 minutes at RT (or 15-25 minutes at 37°C).

Boster Scientific Support

Answered: 2021-01-13

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-08-30

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-08-30

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of IL-2 in Human plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-08-15

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-08-15

Question

Q: if the enzyme conjugated IL-2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IL-2 antigen?

L. Clark

Verified customer

Asked: 2020-06-06

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-06-06

Question

We have a customer that has a question regarding your product EK0397 https://www.bosterbio.com/datasheet_new.php?sku=EK0397 The customer is asking whether the standard can be reused after he has added ddH2O to the standard?

Verified Customer

Verified customer

Asked: 2020-03-13

Answer

We have got two questions for this inquiry. 1. What is the volume of the

Boster Scientific Support

Answered: 2020-03-13

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-08-03

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-08-03

Question

Q: is it okay to use heparin plasma as samples in Human IL-2 Picokine® ELISA Kit (Catalog # EK0397)?

Verified Customer

Verified customer

Asked: 2019-01-12

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of IL-2 causing degradation of its protein structure. IL-2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IL-2 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-01-12

Question

Q: What is the optimal O.D. value for IL-2 ELISA kit? I performed your IL-2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-2 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2017-10-22

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-2 ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of IL-2 that can be declared/interpreted as positive by the above standard is 1pg/ml.

Boster Scientific Support

Answered: 2017-10-22

Question

Q: how to thaw whole blood sample for IL-2 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2017-06-03

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-2 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2017-06-03

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