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Human LIFR/Lif R Alpha ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Human LIFR. 96wells/kit, with removable strips.
Human LIFR/Lif R Alpha ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK1200 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Human |
| Sample Type: | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <10pg/ml |
| Assay Range: | 156pg/ml-10,000pg/ml |
Overview
| Product Name | Human LIFR/Lif R Alpha ELISA Kit PicoKine™
See all LIFR alpha primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK1200 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Human Lif R Alpha/LIFR with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Lif R Alpha/LIFR tissue specificity: |
| Cite This Product | Human LIFR/Lif R Alpha ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1200) |
Product Specs
| Reactivity/Species | Human |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity | <10pg/ml |
| Assay Range | 156pg/ml-10,000pg/ml |
| Immunogen/Standard | Expression system for standard: NSO; Immunogen sequence: Q45-S833 |
| Antibody Clonalities | Capture antibody|Detection antibody polyclonal antibody from goat|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Gene/Protein Basic Information For LIFR (Source: Uniprot.org, NCBI)
| Uniprot Id | P42702 |
|---|---|
| NCBI Gene Id | 3977 |
| Species Of This Entry | Human |
| Gene Name | LIFR |
| Protein Name | Leukemia inhibitory factor receptor |
| Superfamily | type I cytokine receptor family |
| Alternative Names | LIFR alpha|CD118 antigen; CD118; FLJ98106; FLJ99923; leukemia inhibitory factor receptor alpha; leukemia inhibitory factor receptor; LIF R alpha; LIF R beta; LIF receptor; LIFR alpha; LIFR; LIF-R; LIFRa; SJS2; STWS; SWS |
| Molecular Weight | 123743 |
| Post Tranlational Modifications | Phosphorylation; Methylation; Glycosylation; Cleavage; Acetylation; Iodination; Demethylation; Oxidation |
| Subcellular Localization | [Isoform 1]: Cell membrane; Single-pass type I membrane protein.; [Isoform 2]: Secreted. |
| Gene Location | 5p13.1, on Chromasome 5, gene sequence: NC_000005.10 (38474668..38606290, complement) |
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For LIFR (Source: Uniprot.org, NCBI)
| Protein Function | Signal-transducing molecule. May have a common pathway with IL6ST. The soluble form inhibits the biological activity of LIF by blocking its binding to receptors on target cells. |
|---|---|
| Interacting Proteins | CNTF |
| Related Diseases | Leukemia; Neoplasms; Inflammation; Nervousness; Malignant Neoplasms; Janiceps; Endometrial Polyp; Carcinoma; Liver Carcinoma; Hypertrophy; Neuroblastoma; Pituitary Diseases; Myeloid Leukemia; Multiple Myeloma; Malignant Paraganglionic Neoplasm; Choriocarcinoma; Infertility; Malignant Neoplasm Of Breast; Mammary Neoplasms; Chimera Disorder |
| Related Pathways | Secretion; Cell Growth; Cell Proliferation; Regeneration; Cell Differentiation; Cell Death; Embryo Development; Localization; Pathogenesis; Embryo Implantation; Neurogenesis; Inflammatory Response; Reverse Transcription; Bone Resorption; Cell Cycle; Macrophage Differentiation; Osteoblast Differentiation; System Development; Decidualization; Cell Adhesion |
| Background | LIFR, also known as CD118(Cluster of Differentiation 118), is a subunit of a receptor for leukemia inhibitory factor. This gene encodes a protein that belongs to the type I cytokine receptor family. It is mapped to 5p31.1. The LIF receptor(LIFR) is the low-affinity binding chain that, together with the high-affinity converter subunit gp130, forms a high-affinity receptor complex that mediates the action of the leukemia-inhibitory factor. LIF is a polyfunctional cytokine that affects the differentiation, survival, and proliferation of a wide variety of cells in the adult and the embryo. Mutations in this gene cause Schwartz-Jampel syndrome type 2, a disease belonging to the group of the bent-bone dysplasias. A translocation that involves the promoter of this gene, togerther with the pleiomorphic adenoma gene 1, is associated with salivary gland pleiomorphic adenoma, a common type of benign epithelial tumor of the salivary gland. |
| Scientific References | PMID: 1915266 by Gearing D.P., et al. Leukemia inhibitory factor receptor is structurally related to the IL-6 signal transducer, gp130. PMID: 9525740 by Voz M.L., et al. The recurrent translocation t(5;8)(p13;q12) in pleomorphic adenomas results in upregulation of PLAG1 gene expression under control of the LIFR promoter. |
Kit Components And Assay QC Data Details Of Human LIFR/Lif R Alpha ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK1200-CAP | 96-well plate precoated with anti-Human LIFR alpha antibody | 1 |
| EK1200-ST | lyophilized recombinant Human LIFR alpha standard | 10ng/tube |
| EK1200-DA | biotinylated anti-Human LIFR alpha antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1200 Human LIFR/Lif R Alpha ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.047 | 0.159 | 0.255 | 0.432 | 0.770 | 1.308 | 1.980 | 2.392 |
We measured random samples of Human LIFR/Lif R Alpha ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 289 | 1861 | 6421 | 261 | 1769 | 6236 |
| Standard deviation | 16.76 | 91.18 | 276.1 | 16.7 | 107.9 | 386.63 |
| CV(%) | 5.8% | 4.9% | 4.3% | 6.4% | 6.1% | 6.2% |
We measured the performance consistency of Human LIFR/Lif R Alpha ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 289 | 279 | 327 | 287 | 295 | 18.56 | 6.2% |
| Sample 2 | 1861 | 1799 | 1747 | 1540 | 1736 | 120.54 | 6.9% |
| Sample 3 | 6421 | 5493 | 5855 | 5885 | 5913 | 331.13 | 5.6% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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