Human Serpin A6 ELISA Kit PicoKine™

Human Serpin A6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human SERPINA6 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK1963
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA) and urine.

Product Name

Human Serpin A6 ELISA Kit PicoKine™

See all Serpin A6/Cortisol Binding Globulin products

SKU/Catalog Number

EK1963

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human Serpin A6 ELISA Kit PicoKine™ (96 Tests). Quantitate Human SERPINA6 in cell culture supernatants, serum and plasma (heparin, EDTA) and urine. Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human Serpin A6 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1963)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: M23-V405

Sensitivity

<50 pg/ml

Assay Range

1.56 ng/ml - 100 ng/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1963 is reactive to SERPINA6 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA) and urine.

Application Guarantee

EK1963 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1963-CAP Anti-Human SERPINA6 Pre-coated 96-well strip microplate 1
EK1963-ST Human SERPINA6 Standard 2 vials, 100 ng/tube
EK1963-DA Human SERPINA6 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (ng/ml)01.563.126.2512.52550100
O.D.0.1070.1760.2280.3560.6201.0911.7872.314

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Serpin A6/Cortisol Binding Globulin, Dilution ratio of 1:50, concentration in serum is 0.5-1ug/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human Serpin A6 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2728121064302829861233347148
Standard deviation141.86 544.772452.60206.03715.312970.32
CV (%)5.2%4.5%5.7%6.9%5.8%6.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Serpin A6/Cortisol Binding Globulin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 127283062267529542854159.045.5%
Sample 21210613177124571292212665413.503.2%
Sample 343028434274538241055432231536.323.5%
*number of samples for each test n=16.

Gene/Protein Information For SERPINA6 (Source: Uniprot.Org, NCBI)

Uniprot ID

P08185

Gene ID

866

Gene Name

SERPINA6

Full Name

Corticosteroid-binding globulin

Weight

45.141kDa

Superfamily

serpin family

Alternative Names

antitrypsin), member 6; CBG; CBGcorticosteroid-binding globulin; corticosteroid binding globulin; member 6; serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase; Serpin A6; serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin); transcortin SERPINA6 CBG serpin family A member 6 corticosteroid-binding globulin|serine (or cysteine) proteinase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6|serpin A6|serpin peptidase inhibitor, clade A (alpha-1 antiproteinase, antitrypsin), member 6|transcortin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on SERPINA6, check out the SERPINA6 Infographic

SERPINA6 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for SERPINA6: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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17 Customer Q&As for Human Serpin A6 ELISA Kit PicoKine™

Question

What range of values with units are experimentally detectable in human plasma using EK1963?

Verified customer

Asked: 2021-01-13

Answer

For the Human Serpin A6 ELISA Kit PicoKine™ (EK1963), range value in human plasma is 0.1-0.5ug/ml.

Boster Scientific Support

Answered: 2021-01-14

Question

Is EK1963 quantitative?

Verified customer

Asked: 2021-01-13

Answer

The Human Serpin A6 ELISA Kit PicoKine™ (EK1963) is quantitative.

Boster Scientific Support

Answered: 2021-01-13

Question

Is EK1963 pre-coated?

Verified customer

Asked: 2021-01-13

Answer

The Human Serpin A6 ELISA Kit PicoKine™ (EK1963) is pre-coated.

Boster Scientific Support

Answered: 2021-01-13

Question

Has EK1963 been tested/validated on human plasma?

Verified customer

Asked: 2021-01-13

Answer

The Human Serpin A6 ELISA Kit PicoKine™ (EK1963) has been tested on human plasma.

Boster Scientific Support

Answered: 2021-01-13

Question

Has EK1963 been tested/validated on human plasma EDTA?

Verified customer

Asked: 2021-01-13

Answer

The Human Serpin A6 ELISA Kit PicoKine™ (EK1963) has been tested on human plasma EDTA.

Boster Scientific Support

Answered: 2021-01-13

Question

What is the range of values (with units) measured in human plasma specifically (not serum) for EK1963?

Verified customer

Asked: 2021-01-13

Answer

For the Human Serpin A6 ELISA Kit PicoKine™ (EK1963), the range of value is 0.1-1.5ug/ml.

Boster Scientific Support

Answered: 2021-01-13

Question

Do you make EK1963 yourself?

Verified customer

Asked: 2021-01-13

Answer

Yes, we make the Human Serpin A6 ELISA Kit PicoKine™ (EK1963) ourselves.

Boster Scientific Support

Answered: 2021-01-13

Question

Does EK1963 measure total concentration?

Verified customer

Asked: 2021-01-13

Answer

The Human Serpin A6 ELISA Kit PicoKine™ (EK1963) measures total concentration.

Boster Scientific Support

Answered: 2021-01-13

Question

Q: is it okay to use citrate plasma as samples in Human Serpin A6 Picokine® ELISA Kit (Catalog # EK1963)?

Verified Customer

Verified customer

Asked: 2020-09-23

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Serpin A6 causing disruption of its protein structure. Serpin A6 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Serpin A6 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-09-23

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

W. Evans

Verified customer

Asked: 2020-08-06

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2020-08-06

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Serpin A6 using the EK1963 Human Serpin A6 Picokine® ELISA Kit?

P. Walker

Verified customer

Asked: 2020-04-29

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2020-04-29

Question

Q: What is the optimal O.D. value for Serpin A6 ELISA kit? I used your Serpin A6 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain Serpin A6 even though the O.D. values are not very high?

C. Anderson

Verified customer

Asked: 2020-02-25

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Serpin A6 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Serpin A6 that can be declared/interpreted as positive by the above standard is 50pg/ml.

Boster Scientific Support

Answered: 2020-02-25

Question

Q: how do I analyze ELISA data? I measured Serpin A6 level in serum.

Verified Customer

Verified customer

Asked: 2019-08-13

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-08-13

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

B. Miller

Verified customer

Asked: 2019-06-05

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-06-05

Question

Q: if the enzyme conjugated Serpin A6 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Serpin A6 antigen?

C. Nelson

Verified customer

Asked: 2017-12-30

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-12-30

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

G. Collins

Verified customer

Asked: 2017-03-14

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-03-14

Question

Q: which procedure should I follow in order to thaw whole blood sample for Serpin A6 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2016-11-27

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Serpin A6 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2016-11-27

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