Human TGF Alpha ELISA Kit PicoKine®

TGF-alpha ELISA kit for Human

Human TGF Alpha ELISA Kit PicoKine™ (96 Tests). Quantitate Human TGFA in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 1pg/ml. Cited in 34 publication(s).

Product Info Summary

SKU: EK0511
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Product Name

Human TGF Alpha ELISA Kit PicoKine®

View all TGF-alpha ELISA kits

SKU/Catalog Number

EK0511

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TGF Alpha ELISA Kit PicoKine™ (96 Tests). Quantitate Human TGFA in cell culture supernatants, serum, plasma (heparin, EDTA) and human milk. Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human TGF Alpha ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0511)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V40-A89

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.

Reactive Species

EK0511 is reactive to TGFA in Human samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and human milk.

Application Guarantee

EK0511 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TGF-alpha

Transforming growth factor alpha (TGF-alpha) is upregulated in some human cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene (TGFA) maps to human chromosome 2 close to the breakpoint of the t (2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic human TGF-alpha showed similar immunoreactivity when compared with rat TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.

Kit Components

Catalog Number Description Quantity
EK0511-CAP Anti-Human TGFA Pre-coated 96-well strip microplate 1
EK0511-ST Human TGFA Standard 2 vials, 10 ng/tube
EK0511-DA Human TGFA Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0810.1830.2810.4730.8161.1051.5502.047

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect TGF-alpha, Dilution ratio of 1:1, concentration in serum is around 30pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TGF Alpha ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3123044829237431
Standard deviation1.9816.5634.492.418.9634.04
CV (%)6.4%7.2%7.7%8.3%8%7.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of TGF-alpha in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 131333135321.655.1%
Sample 22302192272462309.814.2%
Sample 344849847045446719.354.1%
*number of samples for each test n=16.

Gene/Protein Information For TGFA (Source: Uniprot.Org, NCBI)

Uniprot ID

P01135

Gene ID

7039

Gene Name

TGFA

Full Name

Protransforming growth factor alpha

Weight

17.006kDa

Alternative Names

protransforming growth factor alpha; TFGA; TGFA; TGFalpha; TGF-alpha; transforming growth factor, alpha; transforming growth factor-alpha TGFA TFGA transforming growth factor alpha protransforming growth factor alpha|TGF-alpha

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TGFA, check out the TGFA Infographic

TGFA infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TGFA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0511 has been cited in 34 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Cartilage repair mediated by thermosensitive photocrosslinkable TGFβ1-loaded GM-HPCH via immunomodulating macrophages, recruiting MSCs and promoting chondrogenesis

Effects of the eukaryotic initiation factor 6 gene on expression levels of inflammatory mediators in M2 macrophages during scar repair Retraction in /10.3892/mmr.2017.7190

Flagellin ofPseudomonas aeruginosainduces transforming growth factor beta 1 expression in normal bronchial epithelial cells through mitogen activated protein kinase cascades

In Situ Analysis of Interactions between Fibroblast and Tumor Cells for Drug Assays with Microfluidic Non-Contact Co-Culture

Elevated synovial fluid IL-33 and IL-6 levels and cartilage degeneration in stage III osteonecrosis of the femoral head

Is there a correlation between maternal serum TGF-β1 levels and fetal hydronephrosis?

What is the impact of SOCS3, IL-35 and IL17 in immune pathogenesis of recurrent pregnancy loss?

Platelet-rich plasma plays an antibacterial, anti-inflammatory and cell proliferation-promoting role in an in vitro model for diabetic infected wounds

Estrogens Promote Invasion of Prostate Cancer Cells in a Paracrine Manner through Up-Regulation of Matrix Metalloproteinase 2 in Prostatic Stromal Cells

Possible role of HPV/EBV coinfection in anoikis resistance and development in prostate cancer

The Effect of Autophagic Activity on the Function of Apheresis Platelets and on the Efficacy of Clinical Platelet Transfusion

GABABR-Induced EGFR Transactivation Promotes Migration of Human Prostate Cancer Cells

Effects of Ganoderma lucidum polysaccharides on IEC‐6 cell proliferation, migration and morphology of differentiation benefiting intestinal epithelium healing in vitro

IL-2 and IL-2R gene polymorphisms and immune function in people residing in areas with high background radiation, Yangjiang, China

Clinical features and monocyte/macrophage subsets characterization in granulomatous vs non-granulomatous Crohn’s disease

Arsenic Induced Overexpression of Inflammatory Cytokines Based on the Human Urothelial Cell Model in Vitro and Urinary Secretion of Individuals Chronically Exposed to Arsenic

CPUY192018, a potent inhibitor of the Keap1-Nrf2 protein-protein interaction, alleviates renal inflammation in mice by restricting oxidative stress and NF-κB activation

The impact of Aggregatibacter actinomycetemcomitans biofilm-derived effectors following antimicrobial photodynamic therapy on cytokine production in human gingival fibroblasts

Bufalin targets the SRC-3/MIF pathway in chemoresistant cells to regulate M2 macrophage polarization in colorectal cancer

Shenqi Fuzheng Injection Ameliorates Radiation-induced Brain Injury

Application of decellularized scaffold combined with loaded nanoparticles for heart valve tissue engineering in vitro

Long-term treatment with arsenite activates HER1 and HER2 through upregulating EGF, TGFα, and HSP90 in a human uroepithelial cell line

PDGFRβ-targeted TRAIL specifically induces apoptosis of activated hepatic stellate cells and ameliorates liver fibrosis

Zoledronate dysregulates fatty acid metabolism in renal tubular epithelial cells to induce nephrotoxicity

Respiratory effects induced by occupational exposure to refractory ceramic fibers

CNOT7 depletion reverses natural killer cell resistance by modulating the tumor immune microenvironment of hepatocellular carcinoma

Huang Xq, Zhang Xy, Wang Xr, Yu Sy, Fang Sh, Lu Yb, Zhang Wp, Wei Eq. J Neuroinflammation. 2012 Jun 26;9:145. Doi: 10.1186/1742-2094-9-145. Transforming Growth Factor ??1-Induced Astrocyte Migration Is Mediated In Part By Activating 5-Lipoxygenase...

Li Xn, Pan X, Qiu D. Int J Clin Exp Med. 2014 Dec 15;7(12):5324-9. Ecollection 2014. Imbalances Of Th17 And Treg Cells And Their Respective Cytokines In Copd Patients By Disease Stage.

Chen W, Qi J, Feng F, Wang Md, Bao G, Wang T, Xiang M, Xie Wf. Neurochem Int. 2014 Mar;68:28-37. Doi: 10.1016/J.Neuint.2014.01.015. Epub 2014 Feb 12. Neuroprotective Effect Of Allicin Against Traumatic Brain Injury Via Akt/Endothelial Nitric Oxide...

Ye Xh, Wu Y, Guo Pp, Wang J, Yuan Sy, Shang Y, Yao Sl. Brain Res. 2010 Apr 6;1323:174-83. Doi: 10.1016/J.Brainres.2010.01.079. Epub 2010 Feb 4. Lipoxin A4 Analogue Protects Brain And Reduces Inflammation In A Rat Model Of Focal Cerebral Ischemia R...

Qiu Jl, Zhu Wl, Lu Yj, Bai Zf, Liu Zg, Zhao P, Sun C, Zhang Yb, Li H, Liu W. Neurochem Int. 2015 Jun-Jul;85-86:46-52. Doi: 10.1016/J.Neuint.2015.04.007. Epub 2015 May 4. The Selective Mglur5 Agonist Chpg Attenuates So2-Induced Oxidative Stress And...

Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????

Gao L, Huang W, Li J. Mol Cell Biochem. 2013 Oct;382(1-2):185-91. Doi: 10.1007/S11010-013-1733-4. Epub 2013 Jun 26. Nox1 Abet Mesangial Fibrogenesis Via Inos Induction In Diabetes.

Mao T, Gao L, Li H, Li J. Saudi Med J. 2011 Aug;32(8):769-77. Pigment Epithelium-Derived Factor Inhibits High Glucose Induced Oxidative Stress And Fibrosis Of Cultured Human Glomerular Mesangial Cells.

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7 Customer Q&As for Human TGF Alpha ELISA Kit PicoKine®

Question

Q: how to analyze ELISA data? I have obtained TGF-alpha level in human milk.

J. Surve

Verified customer

Asked: 2019-12-28

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-12-28

Question

Q: if the enzyme conjugated TGF-alpha antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TGF-alpha antigen?

Y. Gandhi

Verified customer

Asked: 2018-04-12

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-04-12

Question

Q: Can TGF-alpha ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Z. Kumar

Verified customer

Asked: 2018-03-14

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-03-14

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-03-06

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-03-06

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2017-06-04

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-06-04

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2016-12-09

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2016-12-09

Question

Q: can you suggest the dilution ratio of serum samples for detection of TGF-alpha in Human human milk? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2014-03-02

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TGF-alpha ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2014-03-02

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