Human TIE2/TEK ELISA Kit PicoKine™

Human TIE2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TEK in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml. Cited in 2 publication(s).

Product Info Summary

SKU: EK0519
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Human TIE2/TEK ELISA Kit PicoKine™

See all Tie-2 products

SKU/Catalog Number

EK0519

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human TIE2 ELISA Kit PicoKine™ (96 Tests). Quantitate Human TEK in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Human TIE2/TEK ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0519)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A23-K745

Sensitivity

<5 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is cross-reactivity with human TIE1< 0.1%.

Reactive Species

EK0519 is reactive to TEK in Human samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0519 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0519-CAP Anti-Human TEK Pre-coated 96-well strip microplate 1
EK0519-ST Human TEK Standard 2 vials, 10 ng/tube
EK0519-DA Human TEK Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010000
O.D.0.0210.0750.1200.2310.4300.8881.6232.371

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human TIE2/TEK ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3051860470431117144949
Standard deviation23.7981.84263.4227.0599.41351.37
CV (%)7.8%4.4%5.6%8.7%5.8%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Tie-2 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 130528929827129012.734.3%
Sample 21860184220151990192676.523.9%
Sample 347045338506051685067232.084.5%
*number of samples for each test n=16.

Gene/Protein Information For TEK (Source: Uniprot.Org, NCBI)

Uniprot ID

Q02763

Gene ID

7010

Gene Name

TEK

Full Name

Angiopoietin-1 receptor

Weight

125.83kDa

Superfamily

protein kinase superfamily

Alternative Names

angiopoietin-1 receptor; CD202b antigen; CD202b; EC 2.7.10; EC 2.7.10.1; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; TEK tyrosine kinase, endothelial; TEK; Tie2; Tie-2; TIE2CD202b; Tunica interna endothelial cell kinase; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; venous malformations, multiple cutaneous and mucosal; VMCM; VMCM1 TEK CD202B, GLC3E, TIE-2, TIE2, VMCM, VMCM1 TEK receptor tyrosine kinase angiopoietin-1 receptor|TEK tyrosine kinase, endothelial|endothelial tyrosine kinase|tunica interna endothelial cell kinase|tyrosine kinase with Ig and EGF homology domains-2|tyrosine-protein kinase receptor TEK|tyrosine-protein kinase receptor TIE-2

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TEK, check out the TEK Infographic

TEK infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TEK: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0519 has been cited in 2 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Effects of laser photocoagulation on serum angiopoietin-1, angiopoietin-2, angiopoietin-1/angiopoietin-2 ratio, and soluble angiopoietin receptor Tie-2 levels in type 2 diabetic patients with proliferative diabetic retinopathy

Sancakdar E, Guven As, Uysal Eb, Deveci K, G??lt??rk E. Pediatr Infect Dis J. 2015 Aug;34(8):E200-5. Doi: 10.1097/Inf.0000000000000706. Important Of Angiopoietic System In Evaluation Of Endothelial Damage In Children With Crimean-Congo Hemorrhagic...

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4 Customer Q&As for Human TIE2/TEK ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated Tie-2 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Tie-2 antigen?

Verified Customer

Verified customer

Asked: 2020-02-03

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-02-03

Question

Q: which procedure should I follow in order to thaw whole blood sample for Tie-2 ELISA after freezing?

K. Li

Verified customer

Asked: 2020-01-06

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Tie-2 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-01-06

Question

Q: how do I analyze ELISA data? I measured Tie-2 level in cell lysates.

Verified Customer

Verified customer

Asked: 2019-10-12

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-10-12

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-09-18

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-09-18

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