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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Human TNFSF13/APRIL ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human TNFSF13/APRIL ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFSF13 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TNFSF13/APRIL ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0921)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A105-L250
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0921 is reactive to TNFSF13 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0921 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of APRIL/TNFSF13
Tumor necrosis factor ligand superfamily member 13 (TNFSF13) also known as a proliferation-inducing ligand (APRIL) is a protein that in humans is encoded by the TNFSF13 gene. TNFSF13 has also been designated CD256 (cluster of differentiation 256). The protein encoded by this gene is a member of the tumor necrosis factor ligand (TNF) ligand family. This protein is a ligand for TNFRSF17/BCMA, a member of the TNF receptor family. This protein and its receptor are both found to be important for B cell development. In vivo experiments suggest an important role for APRIL in the long-term survival of plasma cells in the bone marrow. Mice deficient in April showed a reduced ability to support plasma cell survival In vitro experiments suggested that this protein may be able to induce apoptosis through its interaction with other TNF receptor family proteins such asTNFRSF6/FAS and TNFRSF14/HVEM. Three alternatively spliced transcript variants of this gene encoding distinct isoforms have been reported. The TNFSF13 gene lies 878 bp downstream of the TNFSF12 gene on chromosome 17p13.1.
|EK0921-CAP||Anti-Human TNFSF13 Pre-coated 96-well strip microplate||1|
|EK0921-ST||Human TNFSF13 Standard||2 vials, 10 ng/tube|
|EK0921-DA||Human TNFSF13 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human TNFSF13/APRIL PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect APRIL/TNFSF13, Dilution ratio of 1:1, concentration in serum is N0-90pg/ml.
Some articles we found to cite concentrations of APRIL/TNFSF13 in samples: 17307753 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TNFSF13/APRIL ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of APRIL/TNFSF13 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For TNFSF13 (Source: Uniprot.Org, NCBI)
Tumor necrosis factor ligand superfamily member 13
tumor necrosis factor family
A proliferation-inducing ligand; APRIL; APRILFLJ57090; CD256 antigen; CD256; TALL2; TALL-2; TNF- and APOL-related leukocyte expressed ligand 2; TNF-related death ligand 1; TNFSF13; TRDL1; TRDL-1; tumor necrosis factor (ligand) superfamily, member 13; tumor necrosis factor ligand superfamily member 13; tumor necrosis factor-like protein ZTNF2; tumor necrosis factor-related death ligand-1; ZTNF2 TNFSF13 APRIL, CD256, TALL-2, TALL2, TNLG7B, TRDL-1, UNQ383/PRO715, ZTNF2 TNF superfamily member 13 tumor necrosis factor ligand superfamily member 13|TNF- and APOL-related leukocyte expressed ligand 2|a proliferation-inducing ligand|tumor necrosis factor (ligand) superfamily, member 13|tumor necrosis factor ligand 7B|tumor necrosis factor superfamily member 13|tumor necrosis factor-like protein ZTNF2|tumor necrosis factor-related death ligand-1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on TNFSF13, check out the TNFSF13 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFSF13: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0921 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Prognostic Significance of Serum BAFF, APRIL, TACI and BCMA Levels in Chronic Lymphocytic Leukemia
AB0018 B cell disturbance in rheumatoid arthritis patients: comparative study between treated and non-treated patients
G??zmen S, Karapnar Th, T??fek??i O, Vergin C, Y??ksel F, Irken G, Oren H. Blood Coagul Fibrinolysis. 2014 Jun 6. [Epub Ahead Of Print] B-Cell-Activating Factor, A Proliferation Inducing Ligand And Co-Stimulatory Molecules In The Pathogenesis Of I...
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