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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Human TRAILR3/TNFRSF10C ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Human TRAILR3/TNFRSF10C ELISA Kit PicoKine™ (96 Tests). Quantitate Human TNFRSF10C in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 12pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human TRAILR3/TNFRSF10C ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0533)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A26-A236
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0533 is reactive to TNFRSF10C in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK0533 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TRAILR3/TNFRSF10C
TRAILR3/TNFRSF10C is a member of the TNF-receptor superfamily. This receptor contains an extracellular TRAIL-binding domain and a transmembrane domain, but no cytoplasmic death domain. This receptor is not capable of inducing apoptosis, and is thought to function as an antagonistic receptor that protects cells from TRAIL-induced apoptosis. TRAILR3 gene was found to be a p53-regulated DNA damage-inducible gene. The expression of this gene was detected in many normal tissues but not in most cancer cell lines, which may explain the specific sensitivity of cancer cells to the apoptosis-inducing activity of TRAIL.
|EK0533-CAP||Anti-Human TNFRSF10C Pre-coated 96-well strip microplate||1|
|EK0533-ST||Human TNFRSF10C Standard||2 vials, 10 ng/tube|
|EK0533-DA||Human TNFRSF10C Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Human TRAILR3/ TNFRSF10C PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect TRAILR3/TNFRSF10C, Dilution ratio of 1:1, concentration in serum is around 1ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TRAILR3/TNFRSF10C ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of TRAILR3/TNFRSF10C in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For TNFRSF10C (Source: Uniprot.Org, NCBI)
Tumor necrosis factor receptor superfamily member 10C
Antagonist decoy receptor for TRAIL/Apo-2L; CD263 antigen; CD263; cytotoxic TRAIL receptor-3; DcR1; DCR1MGC149502; Decoy receptor 1; Decoy TRAIL receptor without death domain; LITMGC149501; Lymphocyte inhibitor of TRAIL; TNFRSF10C; TRAIL R3; TRAIL receptor 3; TRAIL receptor without an intracellular domain; TRAILR3; TRAIL-R3; TRAILR3TNF-related apoptosis-inducing ligand receptor 3; TRIDDCR1-TNFR; tumor necrosis factor receptor superfamily member 10C; tumor necrosis factor receptor superfamily, member 10c, decoy without anintracellular domain TNFRSF10C CD263, DCR1, DCR1-TNFR, LIT, TRAIL-R3, TRAILR3, TRID TNF receptor superfamily member 10c tumor necrosis factor receptor superfamily member 10C|TNF-related apoptosis-inducing ligand receptor 3|antagonist decoy receptor for TRAIL/Apo-2L|cytotoxic TRAIL receptor-3|decoy TRAIL receptor without death domain|decoy receptor 1|lymphocyte inhibitor of TRAIL|tumor necrosis factor receptor superfamily, member 10c, decoy without an intracellular domain*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on TNFRSF10C, check out the TNFRSF10C Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFRSF10C: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]terbio.com.
No publications found for EK0533
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