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Product Info Summary
| SKU: | EK0898 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine®
SKU/Catalog Number
EK0898
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine® (96 Tests). Quantitate Human Tryptase/TPSAB1,B2 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0898)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: Met1-Pro275
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0898 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0898 is reactive to Tryptase/TPSAB1,B2 in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0898 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Tryptase/TPSAB1,B2
Tryptases are serine proteases implicated in asthma and are highly expressed in human mast cells. They are derived from at least 4 nonallelic genes clustered on chromosome 16p13.3: TPSAB1, which represents the alpha and beta-I tryptase alleles; TPSB2, which represents the beta-II and beta-III tryptase alleles; TPSG1; and TPSD1. Elevated levels of serum tryptase occur in both anaphylactic and anaphylactoid reactions, but a negative test does not exclude anaphylaxis. Tryptase is less likely to be elevated in food allergy reactions as opposed to other causes of anaphylaxis.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0898-CAP | Anti-Human Tryptase/TPSAB1,B2 Pre-coated 96-well strip microplate | 1 |
| EK0898-ST | Human Tryptase/TPSAB1,B2 Standard | 2 vials, 10 ng/tube |
| EK0898-DA | Human Tryptase/TPSAB1,B2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.067 | 0.172 | 0.232 | 0.368 | 0.62 | 1.013 | 1.576 | 2.125 |
Data Example Images
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Human Tryptase/TPSAB1,B2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Tryptase/TPSAB1,B2, Dilution ratio of 1:1, concentration in serum and plasma is 2.5-5 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 276 | 1887 | 5608 | 283 | 2054 | 5485 |
| Standard deviation | 18.49 | 143.41 | 420.6 | 23.48 | 191.02 | 488.16 |
| CV (%) | 6.7% | 7.6% | 7.5% | 8.3% | 9.3% | 8.9% |
Specific Publications For Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine® (EK0898)
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine®
Question
Could EK0898 also test Alpha?
Verified customer
Asked: 2023-08-10
Answer
Yes, the Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine (EK0898) could also test Alpha.
Boster Scientific Support
Answered: 2023-08-11
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
V. Martin
Verified customer
Asked: 2020-02-06
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may depends upon the kit used so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-02-06
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification TPSB1 using the EK0898 Human TPSB1 Picokine® ELISA Kit?
M. Banerjee
Verified customer
Asked: 2019-10-29
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples in order to prevent protein degradation.
Boster Scientific Support
Answered: 2019-10-29
Question
For EK0898, Does this kit detect all three tryptase types; A , B1 and B2 ?
Verified Customer
Verified customer
Asked: 2019-10-08
Answer
This kit detects all three tryptase types; A , B1 and B2.
Boster Scientific Support
Answered: 2019-10-08
Question
Does EK0898 have cross reactivity to mouse?
Verified customer
Asked: 2019-09-20
Answer
The Human Tryptase/TPSAB1,B2 ELISA Kit PicoKine™ (EK0898) does not cross react with mouse.
Boster Scientific Support
Answered: 2019-09-23
Question
Q: how to thaw whole blood sample for TPSB1 ELISA after freezing?
M. Tiwari
Verified customer
Asked: 2019-03-20
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TPSB1 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-03-20
Question
Q: can you suggest the dilution ratio of serum samples for detection of TPSB1 in Human cell lysates? I am trying to measure a a number of analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
D. Dhar
Verified customer
Asked: 2018-11-30
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TPSB1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/elisa-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-11-30
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2017-01-25
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-01-25
