Human ULBP3 ELISA Kit PicoKine®

ULBP-3 ELISA kit for Human

Human ULBP3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ULBP3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK1686
Size: 96 wells/kit, with removable strips.
Reactive Species: Human
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Product Name

Human ULBP3 ELISA Kit PicoKine®

View all ULBP-3 ELISA kits

SKU/Catalog Number

EK1686

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Human ULBP3 ELISA Kit PicoKine™ (96 Tests). Quantitate Human ULBP3 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Human ULBP3 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1686)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: D30-G217

Sensitivity

<50 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Standard Dilution Instructions

serial dilution instructions image

Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1686 for more details

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1686 is reactive to ULBP3 in Human samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1686 is guaranteed for ELISA in Human by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of ULBP-3

UL16 binding protein 3 (ULBP3) is a cell surface glycoprotein encoded by ULBP3 gene located on the chromosome 6. It is mapped to 6q25.1. ULBP3 is related to MHC class I molecules, but its gene maps outside the MHC locus. The domain structure of ULBP3 differs significantly from those of conventional MHC class I molecules. It does not contain the α3 domain and the transmembrane segment. ULBP3 is thus composed of only the α1α2 domain which is linked to the cell membrane by the GPI anchor. It functions as a stress-induced ligand for NKG2D receptor. The protein encoded by this gene is one of several related ligands of the KLRK1/NKG2D receptor, which is found in primary NK cells. Binding of these ligands to the receptor activates several signal transduction pathways, including the JAK2, STAT5, and ERK pathways. The encoded protein is expressed solubly and on the surface of many tumor cells, making it potentially an important target for therapeutics.

Kit Components

Catalog Number Description Quantity
EK1686-CAP Anti-Human ULBP3 Pre-coated 96-well strip microplate 1
EK1686-ST Human ULBP3 Standard 2 vials, 10 ng/tube
EK1686-DA Human ULBP3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Incubator.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.1480.1890.2280.3190.5730.8391.3842.077

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect ULBP-3, Dilution ratio of 1:1, concentration in serum and plasma is around 260 pg/ml..

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Human ULBP3 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)9847711331014821425
Standard deviation3.9220.5152.125.1526.9984.08
CV (%)4.0%4.3%4.6%5.1%5.6%5.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of ULBP-3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1989810285956.416.7%
Sample 247745949646747413.822.9%
Sample 31133105011161070109233.553.0%
*number of samples for each test n=16.

Gene/Protein Information For ULBP3 (Source: Uniprot.Org, NCBI)

Gene Name

ULBP3

Full Name

UL16-binding protein 3

Weight

27.949kDa

Superfamily

MHC class I family

Alternative Names

NKG2D ligand 3; RAET1N; UL16 binding protein 3; ULBP3; ULBP-3 ULBP3 N2DL-3, NKG2DL3, RAET1N UL16 binding protein 3 UL16-binding protein 3|ALCAN-gamma|NKG2D ligand 3|retinoic acid early transcript 1N

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on ULBP3, check out the ULBP3 Infographic

ULBP3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for ULBP3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].

Hello CJ!

No publications found for EK1686

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Human ULBP3 ELISA Kit PicoKine®?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Human ULBP3 ELISA Kit PicoKine®

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Human ULBP3 ELISA Kit PicoKine®

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis ULBP-3 using the EK1686 Human ULBP-3 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-09-30

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2020-09-30

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

B. Patel

Verified customer

Asked: 2020-09-18

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-09-18

Question

Q: can I use citrate plasma as samples in Human ULBP-3 Picokine® ELISA Kit (Catalog # EK1686)?

Verified Customer

Verified customer

Asked: 2020-05-26

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of ULBP-3 causing degradation of its protein structure. ULBP-3 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the ULBP-3 ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-05-26

Question

Q: how do I analyze ELISA data? I have performed ULBP-3 level in plasma.

Verified Customer

Verified customer

Asked: 2020-05-17

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-05-17

Question

Q: if the enzyme conjugated ULBP-3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the ULBP-3 antigen?

T. Dhawan

Verified customer

Asked: 2019-12-14

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-12-14

Question

Q: What is the optimal O.D. value for ULBP-3 ELISA kit? I performed your ULBP-3 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain ULBP-3 even though the O.D. values are not very high?

T. Thomas

Verified customer

Asked: 2019-02-08

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ULBP-3 ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of ULBP-3 that can be declared/interpreted as positive by the above standard is 50pg/ml.

Boster Scientific Support

Answered: 2019-02-08

Question

Q: how can I thaw whole blood sample for ULBP-3 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-01-20

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test ULBP-3 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-01-20

$499
In stock, 1 left.

Order within 71 hours and 52 minutes to receive by Tue Apr 2

Get A Quote
Eddy test
In stock
Order Product
EK1686
$499.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.