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Product Info Summary
| SKU: | EK1962 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA). |
Product info
Product Name
Human URB/CCDC80 ELISA Kit PicoKine®
SKU/Catalog Number
EK1962
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human URB/CCDC80 ELISA Kit PicoKine® (96 Tests). Quantitate Human CCDC80 in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Human URB/CCDC80 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1962)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: E22-Y950
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
46.9 pg/ml - 3,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1962 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1962 is reactive to CCDC80 in Human samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
Application Guarantee
EK1962 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of CCDC80
Coiled-coil domain-containing protein 80 is a protein that in humans is encoded by the CCDC80 gene. It is mapped to 3q13.2. Bioinformatics analysis suggests the CCDC80 protein is a peroxiredoxin.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1962-CAP | Anti-Human CCDC80 Pre-coated 96-well strip microplate | 1 |
| EK1962-ST | Human CCDC80 Standard | 2 vials, 10 ng/tube |
| EK1962-DA | Human CCDC80 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
| O.D. | 0.021 | 0.086 | 0.145 | 0.276 | 0.535 | 1.014 | 1.701 | 2.472 |
Data Example Images
Click image to see more details
Human URB/CCDC80 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect CCDC80, Dilution ratio of 1:1, concentration in serum and plasma is around 460 pg/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human URB/CCDC80 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 79 | 359 | 1459 | 86 | 438 | 1497 |
| Standard deviation | 3.32 | 24.05 | 80.25 | 5.85 | 25.84 | 91.32 |
| CV (%) | 4.2% | 6.7% | 5.5% | 6.8% | 5.9% | 6.1% |
Specific Publications For Human URB/CCDC80 ELISA Kit PicoKine® (EK1962)
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Customer Reviews
0 Reviews For Human URB/CCDC80 ELISA Kit PicoKine®
Customer Q&As
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9 Customer Q&As for Human URB/CCDC80 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-08-20
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2020-08-20
Question
Q: Can URB ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
C. Sunder
Verified customer
Asked: 2020-08-01
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-08-01
Question
The EK1962 protocol states that the standard needs to be prepared within 2 hours prior the experiment, we have ran out of liofilitzated standards and we want to do another experiment. Would it be better to freeze the reconstitute standard or keep it at 4C?
Verified customer
Asked: 2020-06-18
Answer
For the Human URB/CCDC80 ELISA Kit PicoKine™ (EK1962), we suggest to store the reconstituted standard stock solution at -20°C. Please do not use it after 48 hours.
Boster Scientific Support
Answered: 2020-06-21
Question
Q: if the enzyme conjugated URB antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the URB antigen?
Verified Customer
Verified customer
Asked: 2020-03-11
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2020-03-11
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification URB using the EK1962 Human URB Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-08-25
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to avoid protein degradation.
Boster Scientific Support
Answered: 2019-08-25
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
M. Tiwari
Verified customer
Asked: 2019-07-22
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-07-22
Question
Q: how can I thaw whole blood sample for URB ELISA after freezing?
Verified Customer
Verified customer
Asked: 2018-10-04
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test URB for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-10-04
Question
Q: What is the optimal O.D. value for URB ELISA kit? I used your URB ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain URB even though the O.D. values are not very high?
B. Kapoor
Verified customer
Asked: 2018-01-26
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this URB ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of URB that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-01-26
Question
Q: how do I analyze ELISA data? I measured URB level in plasma.
Verified Customer
Verified customer
Asked: 2015-05-02
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2015-05-02
