Monkey primate TGF Alpha ELISA Kit PicoKine®

TGF-alpha ELISA kit for Monkey

Monkey primate TGF Alpha ELISA Kit PicoKine™ (96 Tests). Quantitate Monkey TGFA in cell culture supernatants, serum, plasma (heparin, EDTA) and monkey primate milk. Sensitivity: 1pg/ml.

Product Info Summary

SKU: EK0511-PR
Size: 96 wells/kit, with removable strips.
Reactive Species: Monkey
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and monkey primate milk.

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Product Name

Monkey primate TGF Alpha ELISA Kit PicoKine®

View all TGF-alpha ELISA kits

SKU/Catalog Number

EK0511-PR

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Monkey primate TGF Alpha ELISA Kit PicoKine™ (96 Tests). Quantitate Monkey TGFA in cell culture supernatants, serum, plasma (heparin, EDTA) and monkey primate milk. Sensitivity: 1pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Monkey primate TGF Alpha ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0511-PR)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: V40-A89

Sensitivity

<1 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross-reactivity

There is cross-reactivity with TGF beta 2, TGF beta 3, TGF beta 5<1%.

Reactive Species

EK0511-PR is reactive to TGFA in Monkey samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and monkey primate milk.

Application Guarantee

EK0511-PR is guaranteed for ELISA in Monkey by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of TGF-alpha

Transforming growth factor alpha (TGF-alpha) is upregulated in some monkey primate cancers. It is produced in macrophages, brain cells, and keratinocytes, and induces epithelial development. It is closely related to EGF, and can also bind to the EGF receptor with similar effects. TGFalpha stimulates neural cell proliferation in the adult injured brain. Transforming growth factor alpha gene (TGFA) maps to monkey primate chromosome 2 close to the breakpoint of the t (2;8) variant translocation in Burkitt lymphoma. Synthetic TGF-alpha was as active as murine epidermal growth factor in binding to the epidermal growth factor receptor and in stimulation of anchorage-dependent and of anchorage-independent growth of normal indicator cells in culture. Synthetic TGF-alpha stimulated plasminogen activator production in A 431 and HeLa cells; the stimulation was similar to that induced by epidermal growth factor. Furthermore, synthetic monkey primate TGF-alpha showed similar immunoreactivity when compared with monkey primate TGF-alpha. Thus, the 50-amino acid TGF-alpha is likely to be the bioactive principle produced and secreted by tumor cell lines.

Kit Components

Catalog Number Description Quantity
EK0511-PR-CAP Anti-Monkey TGFA Pre-coated 96-well strip microplate 1
EK0511-PR-ST Monkey TGFA Standard 2 vials, 10 ng/tube
EK0511-PR-DA Monkey TGFA Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.1020.1990.2910.4430.7111.1561.6762.315

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Monkey primate TGF Alpha ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)118225516126238509
Standard deviation5.4312.8316.58.9514.2827
CV (%)4.65.73.27.165.3

Gene/Protein Information For TGFA (Source: Uniprot.Org, NCBI)

Uniprot ID

P01135

Gene ID

7039

Gene Name

TGFA

Full Name

Protransforming growth factor alpha

Weight

17.006kDa

Alternative Names

protransforming growth factor alpha; TFGA; TGFA; TGFalpha; TGF-alpha; transforming growth factor, alpha; transforming growth factor-alpha TGFA TFGA transforming growth factor alpha protransforming growth factor alpha|TGF-alpha

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on TGFA, check out the TGFA Infographic

TGFA infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for TGFA: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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6 Customer Q&As for Monkey primate TGF Alpha ELISA Kit PicoKine®

Question

Q: Can TGF-alpha ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2018-08-15

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2018-08-15

Question

Q: how can I thaw whole blood sample for TGF-alpha ELISA after freezing?

Verified Customer

Verified customer

Asked: 2018-07-27

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test TGF-alpha for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2018-07-27

Question

Q: if the enzyme conjugated TGF-alpha antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the TGF-alpha antigen?

Verified Customer

Verified customer

Asked: 2018-07-15

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-07-15

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-04-02

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-04-02

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of TGF-alpha in Monkey serum? I am trying to measure a few analytes and it requires 100ul of diluted samples for each well. We have low serum volumes so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2017-07-26

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the TGF-alpha ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2017-07-26

Question

Q: What is the optimal O.D. value for TGF-alpha ELISA kit? I performed your TGF-alpha ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain TGF-alpha even though the O.D. values are not very high?

C. White

Verified customer

Asked: 2017-03-04

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this TGF-alpha ELISA kit to have sensitivity of 1pg/ml, that means the minimum amount of TGF-alpha that can be declared/interpreted as positive by the above standard is 1pg/ml.

Boster Scientific Support

Answered: 2017-03-04

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