Mouse CD36/SR-B3/GP4 ELISA Kit PicoKine™

Mouse CD36/SR-B3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cd36 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0701
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse CD36/SR-B3/GP4 ELISA Kit PicoKine™

See all CD36/SR-B3 products

SKU/Catalog Number

EK0701

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse CD36/SR-B3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Cd36 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse CD36/SR-B3/GP4 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0701)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: CHO; Immunogen sequence: G30-K439

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0701 is reactive to Cd36 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0701 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0701-CAP Anti-Mouse Cd36 Pre-coated 96-well strip microplate 1
EK0701-ST Mouse Cd36 Standard 2 vials, 10 ng/tube
EK0701-DA Mouse Cd36 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010000
O.D.0.0420.1440.2150.3430.5981.0241.7712.326

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect CD36/SR-B3, Dilution ratio of 1:1, concentration in serum is around 150pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse CD36/SR-B3/GP4 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3271000403230810733654
Standard deviation13.7367221.7618.7883.69204.62
CV (%)4.2%6.7%5.5%6.1%7.8%5.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of CD36/SR-B3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 132733229231931715.434.8%
Sample 21000113411461166111165.385.8%
Sample 340323785449542794147265.896.4%
*number of samples for each test n=16.

Gene/Protein Information For Cd36 (Source: Uniprot.Org, NCBI)

Uniprot ID

Q08857

Gene ID

12491

Gene Name

Cd36

Full Name

Platelet glycoprotein 4

Weight

52.698kDa

Superfamily

CD36 family

Alternative Names

CD36 antigen; CD36 molecule (thrombospondin receptor); CD36; Collagen R; FA6-152; FAT; FATCHDS7; Fatty acid translocase; Glycoprotein IIIb; GP3Bthrombospondin receptor); GPIIIb; GPIV; Leukocyte differentiation antigen CD36; PAS IV; PAS-4; Platelet collagen receptor; platelet glycoprotein 4; Platelet glycoprotein IV; SCARB3; scavenger receptor class B, member 3; SRB3; SR-B3; Thrombospondin R; Thrombospondin receptor Cd36|FA, FAT, GPIV, Sca, Scarb3|CD36 molecule|platelet glycoprotein 4|CD36 antigen|GPIIIB|PAS IV|PAS-4|fatty acid translocase|glycoprotein IIIb|platelet glycoprotein IV

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Cd36, check out the Cd36 Infographic

Cd36 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Cd36: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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5 Customer Q&As for Mouse CD36/SR-B3/GP4 ELISA Kit PicoKine™

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

A. Chen

Verified customer

Asked: 2019-12-29

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-12-29

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-08-19

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-08-19

Question

Can EK0701 detect soluble CD36?

Verified Customer

Verified customer

Asked: 2019-06-04

Answer

EK0701 can detect soluble CD36.

Boster Scientific Support

Answered: 2019-06-04

Question

Q: Can CD36 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2017-04-11

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2017-04-11

Question

Q: if the enzyme conjugated CD36 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the CD36 antigen?

Verified Customer

Verified customer

Asked: 2016-05-27

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-05-27

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