Mouse Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine™

Mouse Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ces1d in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK1815
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Product Name

Mouse Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine™

See all Ces1d products

SKU/Catalog Number

EK1815

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Ces1d in cell culture supernatants, serum and plasma (heparin, EDTA, citrate ). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1815)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y19-L565

Sensitivity

<50 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1815 is reactive to Ces1d in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate ).

Application Guarantee

EK1815 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1815-CAP Anti-Mouse Ces1d Pre-coated 96-well strip microplate 1
EK1815-ST Mouse Ces1d Standard 2 vials, 10 ng/tube
EK1815-DA Mouse Ces1d Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0180.1700.2910.6091.1351.7632.1422.383

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Ces1d, Dilution ratio of 1:10, concentration in serum is around 15ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2571095412130312634314
Standard deviation15.9371.2177.215.7677.04310.6
CV (%)6.2%6.5%4.3%5.2%6.1%7.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Ces1d in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 125728228627327411.144.0%
Sample 2109510029691048102847.554.6%
Sample 34121385440054025400195.652.3%
*number of samples for each test n=16.

Gene/Protein Information For Ces1d (Source: Uniprot.Org, NCBI)

Uniprot ID

Q8VCT4

Gene ID

104158

Gene Name

Ces1d

Full Name

Carboxylesterase 1D

Weight

61.788kDa

Superfamily

type-B carboxylesterase/lipase family

Alternative Names

Carboxylesterase 1D Ces1d|Ces, Ces3, TGH|carboxylesterase 1D|carboxylesterase 1D|FAEE synthase|carboxylesterase 3|fatty acid ethyl ester synthase|triacylglycerol hydrolase

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ces1d, check out the Ces1d Infographic

Ces1d infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ces1d: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1815

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6 Customer Q&As for Mouse Carboxylesterase 3/CES3/Ces1d ELISA Kit PicoKine™

Question

Q: how to proceed with the analysis of ELISA data? I have obtained Ces1d level in serum.

Verified Customer

Verified customer

Asked: 2020-02-01

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-02-01

Question

Q: can I use citrate plasma as samples in Mouse Ces1d Picokine® ELISA Kit (Catalog # EK1815)?

K. Chakrabarti

Verified customer

Asked: 2019-12-15

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of Ces1d causing disruption of its protein structure. Ces1d may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the Ces1d ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-12-15

Question

Q: Can Ces1d ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-09-17

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-09-17

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-05-19

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-05-19

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Ces1d using the EK1815 Mouse Ces1d Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-03-01

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to avoid protein degradation.

Boster Scientific Support

Answered: 2019-03-01

Question

Q: What is the optimal O.D. value for Ces1d ELISA kit? I performed your Ces1d ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Ces1d even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-06-10

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Ces1d ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of Ces1d that can be declared/interpreted as positive by the above standard is 50pg/ml.

Boster Scientific Support

Answered: 2018-06-10

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