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Product Info Summary
| SKU: | EK2081 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse DNER ELISA Kit PicoKine®
SKU/Catalog Number
EK2081
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse DNER ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Dner in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 50pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse DNER ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2081)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: A26-Y640
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<50 pg/ml
Assay Range
312 pg/ml - 20,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2081 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2081 is reactive to Dner in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK2081 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Dner
Delta and Notch-like epidermal growth factor-related receptor is a protein that in humans is encoded by the DNER gene. It is mapped to 1; 1 C5. DNER, also known as BET, is a type I transmembrane glycoprotein that is specifically expressed on nonaxonal areas of post-mitotic neurons. The protein has an extracellular domain containing ten distinct EGF-like repeats similar to those found on Delta and Notch. Human and mouse DNER share 90% amino acid sequence identity.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2081-CAP | Anti-Mouse Dner Pre-coated 96-well strip microplate | 1 |
| EK2081-ST | Mouse Dner Standard | 2 vials, 20 ng/tube |
| EK2081-DA | Mouse Dner Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (ng/ml) | 0 | 0.312 | 0.62 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.006 | 0.037 | 0.071 | 0.14 | 0.253 | 0.483 | 0.922 | 1.81 |
Data Example Images
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Mouse DNER PicoKine ELISA Kit Standard Curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse DNER ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 362 | 1554 | 8817 | 431 | 1816 | 9475 |
| Standard deviation | 17.74 | 82.36 | 476.12 | 28.45 | 123.49 | 748.53 |
| CV (%) | 4.9% | 5.3% | 5.4% | 6.6% | 6.8% | 7.9% |
Specific Publications For Mouse DNER ELISA Kit PicoKine® (EK2081)
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Customer Reviews
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Customer Q&As
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5 Customer Q&As for Mouse DNER ELISA Kit PicoKine®
Question
Q: What is the optimal O.D. value for DNER ELISA kit? I performed your DNER ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain DNER even though the O.D. values are not very high?
L. Davis
Verified customer
Asked: 2020-11-03
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this DNER ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of DNER that can be declared/interpreted as positive by the above standard is 50pg/ml.
Boster Scientific Support
Answered: 2020-11-03
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
A. Lopez
Verified customer
Asked: 2018-11-29
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-11-29
Question
Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?
L. Rodriguez
Verified customer
Asked: 2018-11-28
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-11-28
Question
Q: is it okay to use heparin plasma as samples in Mouse DNER Picokine® ELISA Kit (Catalog # EK2081)?
J. Thomas
Verified customer
Asked: 2016-09-16
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of DNER causing disruption of its protein structure. DNER may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes a must for detection and block the antigen antibody reaction. We have tested the DNER ELISA, treating samples with different anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2016-09-16
Question
Q: if the enzyme conjugated DNER antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the DNER antigen?
Verified Customer
Verified customer
Asked: 2015-01-09
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2015-01-09
