Mouse Endothelin 1/EDN1 ELISA Kit PicoKine™

Mouse Endothelin 1/EDN1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Edn1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml. Cited in 3 publication(s).

Product Info Summary

SKU: EK0953
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse Endothelin 1/EDN1 ELISA Kit PicoKine™

See all Endothelin-1 products

SKU/Catalog Number

EK0953

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Endothelin 1/EDN1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Edn1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 0.5pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse Endothelin 1/EDN1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0953)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: human peptide; Immunogen sequence: C53-W73

Sensitivity

<0.5 pg/ml

Assay Range

3.9 pg/ml - 250 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0953 is reactive to Edn1 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0953 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0953-CAP Anti-Mouse Edn1 Pre-coated 96-well strip microplate 1
EK0953-ST Mouse Edn1 Standard 2 vials, 1 ng/tube
EK0953-DA Mouse Edn1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03.97.815.631.262.5125250
O.D.0.0010.1070.2060.3580.7011.2811.8302.192

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Endothelin 1/EDN1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)1952101185495
Standard deviation1.253.694.041.334.154.94
CV (%)6.6%7.1%4%7.4%7.7%5.2%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Endothelin-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 119191817180.824.5%
Sample 252526159564.067.2%
Sample 31011061111011044.143.9%
*number of samples for each test n=16.

Gene/Protein Information For Edn1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P22387

Gene ID

13614

Gene Name

Edn1

Full Name

Endothelin-1

Weight

22.809kDa

Superfamily

endothelin/sarafotoxin family

Alternative Names

ARCND3; EDN1; endothelin 1; Endothelin-1; ET1; HDLCQ7; PPET1; preproendothelin-1; QME Edn1|ET-1, PPET1, preproET|endothelin 1|endothelin-1|preproendothelin-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Edn1, check out the Edn1 Infographic

Edn1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Edn1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0953 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Fu DN,Wang Y,Yu LJ, Liu MJ,Zhen D.Silenced long non-coding RNA activated by DNA damage elevates microRNA-495-3p to suppress atherosclerotic plaque formation via reducing Krüppel-like factor 5.Exp Cell Res.2021 Feb 23: 112519.doi:10.1016/j.yexcr.2021.11251
Species: Mouse
EK0953 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Peng Hj, Dai Dz, Ji H, Dai Y. Eur J Pharmacol. 2010 May 25;634(1-3):101-6. Doi: 10.1016/J.Ejphar.2010.02.001. Epub 2010 Feb 16. The Separate Roles Of Endothelin Receptors Participate In Remodeling Of Matrix Metalloproteinase And Connexin 43 Of Car...

Liu B, Zhu Y, Zhou J, Wei Y, Long C, Chen M, Ling Y, Ge J, Zhuo Y. Cell Stress Chaperones. 2014 Nov;19(6):827-35. Doi: 10.1007/S12192-014-0506-7. Epub 2014 Mar 19. Endoplasmic Reticulum Stress Promotes Amyloid-Beta Peptides Production In Rgc-5 Cells.

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10 Customer Q&As for Mouse Endothelin 1/EDN1 ELISA Kit PicoKine™

Question

Q: can I use citrate plasma as samples in Mouse Endothelin-1 Picokine® ELISA Kit (Catalog # EK0953)?

Verified Customer

Verified customer

Asked: 2020-11-29

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Endothelin-1 causing disruption of its protein structure. Endothelin-1 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Endothelin-1 ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-11-29

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Endothelin-1 using the EK0953 Mouse Endothelin-1 Picokine® ELISA Kit?

M. Young

Verified customer

Asked: 2020-06-18

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2020-06-18

Question

Q: how do I analyze ELISA data? I have obtained Endothelin-1 level in plasma.

Verified Customer

Verified customer

Asked: 2020-04-25

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-04-25

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

L. Sunder

Verified customer

Asked: 2020-02-20

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2020-02-20

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

C. Smith

Verified customer

Asked: 2020-02-02

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-02-02

Question

Q: can you suggest the dilution ratio of serum samples for detection of Endothelin-1 in Mouse plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2019-12-12

Answer

A: having little idea about the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Endothelin-1 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-12-12

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

B. Anand

Verified customer

Asked: 2018-10-25

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can effect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-10-25

Question

Q: Can Endothelin-1 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

K. Lopez

Verified customer

Asked: 2017-10-11

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2017-10-11

Question

Q: if the enzyme conjugated Endothelin-1 antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Endothelin-1 antigen?

Verified Customer

Verified customer

Asked: 2016-02-11

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2016-02-11

Question

Q: which procedure should I follow in order to thaw whole blood sample for Endothelin-1 ELISA after freezing?

Verified Customer

Verified customer

Asked: 2015-10-09

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Endothelin-1 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2015-10-09

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