Mouse EPO/Erythropoietin ELISA Kit PicoKine™

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Mouse EPO Elisa kit has no corss reactivity with human samples, but it will cross react with rat samples.

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A. This kit can detect eosinophils in mice intestine homogenates if the target is in the detectable range. A pilot study is recommended.

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Please refer to the following post: https://www.researchgate.net/post/How_can_I_convert_ng_mL_to_mIU_mL_of_EPO

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We have not validated this kit with EDTA-treated plasma and don’t know if it will work well.

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A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

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A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Erythropoietin ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of Erythropoietin that can be declared/interpreted as positive by the above standard is 15pg/ml.

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A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

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A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Erythropoietin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

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A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

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A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

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A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

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Other components in Mouse EPO/Erythropoietin ELISA Kit PicoKine don't contain any recombinant proteins except the mouse EPO Standard.

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Our lab technicians haven't worked on rabbit samples using the Mouse EPO/Erythropoietin ELISA Kit PicoKine™ (EK0333). The immunogen sequence of the antibodies in the kit is A27-R192. The mouse sequence shares 77.8% homology with rabbit EPO. The kit is not likely to work on rabbit samples on the basis of sequence homology.