Product Info Summary
| SKU: | EK0333 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin). |
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Product info
Product Name
Mouse EPO/Erythropoietin ELISA Kit PicoKine®
View all Erythropoietin/EPO ELISA kits
SKU/Catalog Number
EK0333
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse EPO/Erythropoietin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Epo in cell culture supernatants, serum and plasma (heparin). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse EPO/Erythropoietin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0333)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: A27-R192
Sensitivity
<15 pg/ml
Assay Range
46.9 pg/ml - 3,000 pg/ml
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0333 is reactive to Epo in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
Application Guarantee
EK0333 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Erythropoietin/EPO
Erythropoietin, EPO, also known as hematopoietin or hemopoietin, is a glycoprotein hormone that controls erythropoiesis, or red blood cell production. It is a cytokine for erythrocyte (red blood cell) precursors in the bone marrow. Its gene is mapped to 7q22. It is said that the EPO gene encodes a deduced 193-amino acid propolypeptide. This hormone can be found in kidney and liver. It is the hormone that regulates red blood cell production. And it plays an important role in the brain's response to neuronal injury. What's more, EPO is also involved in the wound healing process.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0333-CAP | Anti-Mouse Epo Pre-coated 96-well strip microplate | 1 |
| EK0333-ST | Mouse Epo Standard | 2 vials, 10 ng/tube |
| EK0333-DA | Mouse Epo Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
| O.D. | 0.053 | 0.126 | 0.204 | 0.346 | 0.555 | 0.875 | 1.385 | 1.999 |
Data Example Images
Click image to see more details
Mouse EPO PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Erythropoietin/EPO, Dilution ratio of 1:1, concentration in serum is 300-700pg/ml.
Some articles we found to cite concentrations of Erythropoietin/EPO in samples: 25395194 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse EPO/Erythropoietin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 111 | 307 | 1298 | 106 | 278 | 1216 |
| Standard deviation | 7.87 | 17.19 | 55.81 | 7.86 | 16.68 | 71.74 |
| CV (%) | 7.1% | 5.6% | 4.3% | 7.8% | 6% | 5.9% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of Erythropoietin/EPO in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 111 | 109 | 96 | 102 | 104 | 5.93 | 5.7% |
| Sample 2 | 307 | 322 | 308 | 317 | 313 | 6.26 | 2% |
| Sample 3 | 1298 | 1321 | 1280 | 1385 | 1321 | 39.7 | 3% |
Protein Target Info & Infographic
Gene/Protein Information For Epo (Source: Uniprot.Org, NCBI)
Uniprot ID
P07321
Gene ID
13856
Gene Name
Epo
Full Name
Erythropoietin
Weight
21.365kDa
Superfamily
EPO/TPO family
Alternative Names
ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2 Epo|erythropoietin|erythropoietin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Epo, check out the Epo Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Epo: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Mouse EPO/Erythropoietin ELISA Kit PicoKine® (EK0333)
EK0333 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Effects of ferulic acid on hematopoietic cell recovery in whole-body gamma irradiated mice
Improvement of icaritin on hematopoietic function in cyclophosphamide-induced myelosuppression mice
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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Submit your question
12 Customer Q&As for Mouse EPO/Erythropoietin ELISA Kit PicoKine®
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Erythropoietin using the EK0333 Mouse Erythropoietin Picokine® ELISA Kit?
W. Patel
Verified customer
Asked: 2020-11-26
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-11-26
Question
Q: What is the optimal O.D. value for Erythropoietin ELISA kit? I used your Erythropoietin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Erythropoietin even though the O.D. values are not very high?
K. Davis
Verified customer
Asked: 2020-08-24
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Erythropoietin ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of Erythropoietin that can be declared/interpreted as positive by the above standard is 15pg/ml.
Boster Scientific Support
Answered: 2020-08-24
Question
Do other components in EK0333 contain any recombinant proteins?
Verified customer
Asked: 2019-12-26
Answer
Other components in the Mouse EPO/Erythropoietin ELISA Kit PicoKine® (EK0333) don't contain any recombinant proteins except the mouse EPO Standard.
Boster Scientific Support
Answered: 2019-12-30
Question
Can you comment on potential cross-reactivity of Erythropoietin (EPO) Mouse ELISA Kit EK0333 with rat and human samples, please? Or any other species that you might have tested with the kit.
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
Mouse EPO Elisa kit has no corss reactivity with human samples, but it will cross react with rat samples.
Boster Scientific Support
Answered: 2019-09-30
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
J. Walker
Verified customer
Asked: 2018-05-26
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2018-05-26
Question
Q: which procedure should I follow in order to thaw whole blood sample for Erythropoietin ELISA after freezing?
H. Hill
Verified customer
Asked: 2018-01-17
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Erythropoietin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-01-17
Question
Can I detect eosinophils in mice intestine homogenates?
Verified Customer
Verified customer
Asked: 2017-10-02
Answer
A. This kit can detect eosinophils in mice intestine homogenates if the target is in the detectable range. A pilot study is recommended.
Boster Scientific Support
Answered: 2017-10-02
Question
How do I convert international units to mass units for EPO? Keyword: conversion units
Verified Customer
Verified customer
Asked: 2016-09-30
Answer
Please refer to the following post: https://www.researchgate.net/post/How_can_I_convert_ng_mL_to_mIU_mL_of_EPO
Boster Scientific Support
Answered: 2016-09-30
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2016-06-20
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-06-20
Question
Is it okay to use EDTA-treaded blood/RBC instead of heparin-treated blood/RBC?
Verified Customer
Verified customer
Asked: 2015-01-22
Answer
We have not validated this kit with EDTA-treated plasma and don’t know if it will work well.
Boster Scientific Support
Answered: 2015-01-22
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2014-09-21
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2014-09-21
Question
Q: if the enzyme conjugated Erythropoietin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Erythropoietin antigen?
Verified Customer
Verified customer
Asked: 2014-09-12
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2014-09-12
