Overview
Product Name |
Mouse EPO/Erythropoietin ELISA Kit PicoKine™
See more Erythropoietin/EPO products |
Catalog# |
EK0333 |
Storage & Handling |
Store at -20˚C for one year from date of receipt. After reconstitution, at 4˚C for one month. It can also be aliquotted and stored frozen at -20˚C for six months. Avoid repeated freeze-thaw cycles. |
Description |
Mouse EPO/Erythropoietin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Epo in cell culture supernatants, serum and plasma (heparin).. Sensitivity: 15pg/ml. |
Cite This Product |
Mouse EPO/Erythropoietin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0333)
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ELISA Validation |
ELISA Validation Information
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Product Specs
Reactivity/Species |
Mouse |
Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
Sample Type |
cell culture supernatants, serum and plasma (heparin). |
Sensitivity |
<15 pg/ml |
Assay Range |
46.9 pg/ml - 3,000 pg/ml |
Immunogen/Standard |
Expression system for standard: NS0; Immunogen sequence: A27-R192 |
Antibody Clonalities |
Capture antibody|Detection antibody monoclonal antibody from rat|monoclonal antibody from rat |
Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
Pack Size |
96 wells/kit, with removable strips. |
Gene/Protein Basic Information For Epo (Source: Uniprot.org, NCBI)
Uniprot Id | P07321 |
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NCBI Gene Id | 13856 |
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Species Of This Entry | Mouse |
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Gene Name | Epo |
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Protein Name | Erythropoietin |
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Superfamily | EPO/TPO family |
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Alternative Names | Erythropoietin/EPO|ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2 |
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Molecular Weight | 21365 |
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*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Epo, check out the Epo Infographic
We have 30,000+ of these available, one for each gene! Check them out.
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Epo: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
Take me to the Epo infographic
Kit Components And Assay QC Data Details Of Mouse EPO/Erythropoietin ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
Catalog number | Description | Quantity |
EK0333-CAP |
Anti-Mouse Epo Pre-coated 96-well strip microplate | 1 |
EK0333-ST | Mouse Epo Standard | 2 vials, 10 ng/tube |
EK0333-DA | Mouse Epo Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
This data is generated from a recent batch of EK0333 Mouse EPO/Erythropoietin ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
Concentration (pg/ml) | 0 | 46.9 | 93.8 | 187.5 | 375 | 750 | 1500 | 3000 |
O.D. | 0.053 | 0.104 | 0.146 | 0.255 | 0.475 | 0.885 | 1.699 | 2.595 |
We measured random samples of Mouse EPO/Erythropoietin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
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Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 111 | 307 | 1298 | 106 | 278 | 1216 |
Standard deviation | 7.87 | 17.19 | 55.81 | 7.86 | 16.68 | 71.74 |
CV (%) | 7.1% | 5.6% | 4.3% | 7.8% | 6% | 5.9% |
We measured the performance consistency of Mouse EPO/Erythropoietin ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
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Sample 1 | 111 | 109 | 96 | 102 | 104 | 5.93 | 5.7% |
Sample 2 | 307 | 322 | 308 | 317 | 313 | 6.26 | 2% |
Sample 3 | 1298 | 1321 | 1280 | 1385 | 1321 | 39.7 | 3% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Other Recommended Resources
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Total number of citations: 0
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Questions and answers from customers related to EK0333 Mouse EPO/Erythropoietin ELISA Kit PicoKine™
13 Related Questions
Question
Can you comment on potential cross-reactivity of Erythropoietin (EPO) Mouse ELISA Kit EK0333 with rat and human samples, please? Or any other species that you might have tested with the kit.
Verified Customer
Asked: 2019-09-30
Answer
Mouse EPO Elisa kit has no corss reactivity with human samples, but it will cross react with rat samples.
Boster Scientific Support
Answered: 2019-09-30
Question
Can I detect eosinophils in mice intestine homogenates?
Verified Customer
Asked: 2017-10-02
Answer
A. This kit can detect eosinophils in mice intestine homogenates if the target is in the detectable range. A pilot study is recommended.
Boster Scientific Support
Answered: 2017-10-02
Question
How do I convert international units to mass units for EPO?
Keyword: conversion units
Verified Customer
Asked: 2016-09-30
Answer
Please refer to the following post:
https://www.researchgate.net/post/How_can_I_convert_ng_mL_to_mIU_mL_of_EPO
Boster Scientific Support
Answered: 2016-09-30
Question
Is it okay to use EDTA-treaded blood/RBC instead of heparin-treated blood/RBC?
Verified Customer
Asked: 2015-01-22
Answer
We have not validated this kit with EDTA-treated plasma and don’t know if it will work well.
Boster Scientific Support
Answered: 2015-01-22
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Erythropoietin using the EK0333 Mouse Erythropoietin Picokine® ELISA Kit?
W. Patel
Asked: 2020-11-26
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2020-11-26
Question
Q: What is the optimal O.D. value for Erythropoietin ELISA kit? I used your Erythropoietin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Erythropoietin even though the O.D. values are not very high?
K. Davis
Asked: 2020-08-24
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Erythropoietin ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of Erythropoietin that can be declared/interpreted as positive by the above standard is 15pg/ml.
Boster Scientific Support
Answered: 2020-08-24
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
J. Walker
Asked: 2018-05-26
Boster Scientific Support
Answered: 2018-05-26
Question
Q: which procedure should I follow in order to thaw whole blood sample for Erythropoietin ELISA after freezing?
H. Hill
Asked: 2018-01-17
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Erythropoietin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2018-01-17
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Asked: 2016-06-20
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2016-06-20
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Asked: 2014-09-21
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2014-09-21
Question
Q: if the enzyme conjugated Erythropoietin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Erythropoietin antigen?
Verified Customer
Asked: 2014-09-12
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2014-09-12
Question
Do product EK0333 other components contain any recombinant proteins?
Answer
Other components in Mouse EPO/Erythropoietin ELISA Kit PicoKine don't contain any recombinant proteins except the mouse EPO Standard.
Boster Scientific Support
Answered: 2019-12-30
Question
Will EK0333 work with rabbit samples?
Answer
Our lab technicians haven't worked on rabbit samples using the Mouse EPO/Erythropoietin ELISA Kit PicoKine™ (EK0333).
The immunogen sequence of the antibodies in the kit is A27-R192.
The mouse sequence shares 77.8% homology with rabbit EPO.
The kit is not likely to work on rabbit samples on the basis of sequence homology.
Boster Scientific Support
Answered: 2019-04-09