Mouse EPO/Erythropoietin ELISA Kit PicoKine™

Mouse EPO/Erythropoietin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Epo in cell culture supernatants, serum and plasma (heparin). Sensitivity: 15pg/ml.

Product Info Summary

SKU: EK0333
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Mouse EPO/Erythropoietin ELISA Kit PicoKine™

See all Erythropoietin/EPO products

SKU/Catalog Number

EK0333

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse EPO/Erythropoietin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Epo in cell culture supernatants, serum and plasma (heparin). Sensitivity: 15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse EPO/Erythropoietin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0333)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A27-R192

Sensitivity

<15 pg/ml

Assay Range

46.9 pg/ml - 3,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0333 is reactive to Epo in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK0333 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0333-CAP Anti-Mouse Epo Pre-coated 96-well strip microplate 1
EK0333-ST Mouse Epo Standard 2 vials, 10 ng/tube
EK0333-DA Mouse Epo Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)046.993.8187.537575015003000
O.D.0.0530.1260.2040.3460.5550.8751.3851.999

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Erythropoietin/EPO, Dilution ratio of 1:1, concentration in serum is 300-700pg/ml.

Some articles we found to cite concentrations of Erythropoietin/EPO in samples: 25395194 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse EPO/Erythropoietin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)11130712981062781216
Standard deviation7.8717.1955.817.8616.6871.74
CV (%)7.1%5.6%4.3%7.8%6%5.9%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Erythropoietin/EPO in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 1111109961021045.935.7%
Sample 23073223083173136.262%
Sample 31298132112801385132139.73%
*number of samples for each test n=16.

Gene/Protein Information For Epo (Source: Uniprot.Org, NCBI)

Uniprot ID

P07321

Gene ID

13856

Gene Name

Epo

Full Name

Erythropoietin

Weight

21.365kDa

Superfamily

EPO/TPO family

Alternative Names

ECYT5; EP; EPO; epoetin; Erythropoietin; MGC138142; MVCD2 Epo|erythropoietin|erythropoietin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Epo, check out the Epo Infographic

Epo infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Epo: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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13 Customer Q&As for Mouse EPO/Erythropoietin ELISA Kit PicoKine™

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Erythropoietin using the EK0333 Mouse Erythropoietin Picokine® ELISA Kit?

W. Patel

Verified customer

Asked: 2020-11-26

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2020-11-26

Question

Q: What is the optimal O.D. value for Erythropoietin ELISA kit? I used your Erythropoietin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Erythropoietin even though the O.D. values are not very high?

K. Davis

Verified customer

Asked: 2020-08-24

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Erythropoietin ELISA kit to have sensitivity of 15pg/ml, that means the minimum amount of Erythropoietin that can be declared/interpreted as positive by the above standard is 15pg/ml.

Boster Scientific Support

Answered: 2020-08-24

Question

Do product EK0333 other components contain any recombinant proteins?

Verified customer

Asked: 2019-12-26

Answer

Other components in Mouse EPO/Erythropoietin ELISA Kit PicoKine don't contain any recombinant proteins except the mouse EPO Standard.

Boster Scientific Support

Answered: 2019-12-30

Question

Can you comment on potential cross-reactivity of Erythropoietin (EPO) Mouse ELISA Kit EK0333 with rat and human samples, please? Or any other species that you might have tested with the kit.

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

Mouse EPO Elisa kit has no corss reactivity with human samples, but it will cross react with rat samples.

Boster Scientific Support

Answered: 2019-09-30

Question

Will EK0333 work with rabbit samples?

Verified customer

Asked: 2019-04-01

Answer

Our lab technicians haven't worked on rabbit samples using the Mouse EPO/Erythropoietin ELISA Kit PicoKine™ (EK0333). The immunogen sequence of the antibodies in the kit is A27-R192. The mouse sequence shares 77.8% homology with rabbit EPO. The kit is not likely to work on rabbit samples on the basis of sequence homology.

Boster Scientific Support

Answered: 2019-04-09

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

J. Walker

Verified customer

Asked: 2018-05-26

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-05-26

Question

Q: which procedure should I follow in order to thaw whole blood sample for Erythropoietin ELISA after freezing?

H. Hill

Verified customer

Asked: 2018-01-17

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Erythropoietin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2018-01-17

Question

Can I detect eosinophils in mice intestine homogenates?

Verified Customer

Verified customer

Asked: 2017-10-02

Answer

A. This kit can detect eosinophils in mice intestine homogenates if the target is in the detectable range. A pilot study is recommended.

Boster Scientific Support

Answered: 2017-10-02

Question

How do I convert international units to mass units for EPO? Keyword: conversion units

Verified Customer

Verified customer

Asked: 2016-09-30

Answer

Please refer to the following post: https://www.researchgate.net/post/How_can_I_convert_ng_mL_to_mIU_mL_of_EPO

Boster Scientific Support

Answered: 2016-09-30

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2016-06-20

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2016-06-20

Question

Is it okay to use EDTA-treaded blood/RBC instead of heparin-treated blood/RBC?

Verified Customer

Verified customer

Asked: 2015-01-22

Answer

We have not validated this kit with EDTA-treated plasma and don’t know if it will work well.

Boster Scientific Support

Answered: 2015-01-22

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2014-09-21

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2014-09-21

Question

Q: if the enzyme conjugated Erythropoietin antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Erythropoietin antigen?

Verified Customer

Verified customer

Asked: 2014-09-12

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2014-09-12

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