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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Mouse ESM1/Endocan ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse ESM1/Endocan ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Esm1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse ESM1/Endocan ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0753)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: W20-R184
23.4 pg/ml - 1500 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0753 is reactive to Esm1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0753 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0753-CAP||Anti-Mouse Esm1 Pre-coated 96-well strip microplate||1|
|EK0753-ST||Mouse Esm1 Standard||2 vials, 10 ng/tube|
|EK0753-DA||Mouse Esm1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse ESM1/Endocan PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Endocan/ESM-1, Dilution ratio of 1:1, concentration in serum is 400-2000pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse ESM1/Endocan ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Endocan/ESM-1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Esm1 (Source: Uniprot.Org, NCBI)
Endothelial cell-specific molecule 1
Endocan; endothelial cell-specific molecule 1; ESM1; ESM-1; IGFBP-rp6 Esm1|0610042H23Rik, AV004503, ESM-1|endothelial cell-specific molecule 1|endothelial cell-specific molecule 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Esm1, check out the Esm1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Esm1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0753 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
ESM1 mediates NGFR-induced invasion and metastasis in murine oral squamous cell carcinoma
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