Mouse FCRL1 ELISA Kit PicoKine®

FCRLA/FCRLM1 ELISA kit for Mouse

Mouse FCRL1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Fcrla in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Product Info Summary

SKU: EK1977
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA).

Customers Who Bought This Also Bought

Product Name

Mouse FCRL1 ELISA Kit PicoKine®

View all FCRLA/FCRLM1 ELISA kits

SKU/Catalog Number

EK1977

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse FCRL1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Fcrla in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 50pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse FCRL1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1977)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: A17-S219

Sensitivity

<50 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1977 is reactive to Fcrla in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA).

Application Guarantee

EK1977 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of FCRLA/FCRLM1

Fc receptor-like protein 1 is a protein that in humans is encoded by the FCRL1 gene. It is mapped to 3; 3 F1.This gene encodes a member of the immunoglobulin receptor superfamily and is one of several Fc receptor-like glycoproteins clustered on the long arm of chromosome 1. The encoded protein contains three extracellular C2-like immunoglobulin domains, a transmembrane domain and a cytoplasmic domain with two immunoreceptor-tyrosine activation motifs. This protein may play a role in the regulation of cancer cell growth. Alternative splicing results in multiple transcript variants.

Kit Components

Catalog Number Description Quantity
EK1977-CAP Anti-Mouse Fcrla Pre-coated 96-well strip microplate 1
EK1977-ST Mouse Fcrla Standard 2 vials, 10 ng/tube
EK1977-DA Mouse Fcrla Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0410.0960.1660.2960.5831.0271.6742.305

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect FCRLA/FCRLM1, Dilution ratio of 1:1, concentration in serum is around 200pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse FCRL1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)904641704964761814
Standard deviation4.4121.8169.865.3824.2883.44
CV (%)4.9%4.7%4.1%5.6%5.1%4.6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of FCRLA/FCRLM1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 190968588894.024.5%
Sample 246440947642044228.346.4%
Sample 31704161715951596162844.742.7%
*number of samples for each test n=16.

Gene/Protein Information For Fcrla (Source: Uniprot.Org, NCBI)

Uniprot ID

Q920A9

Gene ID

98752

Gene Name

Fcrla

Full Name

Fc receptor-like A

Weight

38.348kDa

Alternative Names

Fc receptor homolog expressed in B cells (FREB); Fc receptor homolog expressed in B-cells; Fc receptor related protein X; Fc receptor-like A; Fc receptor-like and mucin-like 1; Fc receptor-related protein X; FCRL1; FCRLA; FCRLb; FCRLc1; FCRLc2; FCRLd; FCRLe; FCRLFc receptor-like protein; FCRLM1; FCRLX; FcRX; FREB; FREBFc receptor-like and mucin-like protein 1; MGC4595 Fcrla|BB219290, FCR, FCRL, FCRL1, FRE, FREB, Fcrlm1, Fcrx, Freb1, mFRE, mFREB, mFcr, mFcrX|Fc receptor-like A|Fc receptor-like A|Fc receptor related X|Fc receptor related protein X|Fc receptor-like mucin-like 1|fc receptor homolog expressed in B-cells|fc receptor-like and mucin-like protein 1|fc receptor-like protein|fc receptor-related protein X

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Fcrla, check out the Fcrla Infographic

Fcrla infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Fcrla: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1977

*Do you have publications using this product? Share with us and receive a reward. Ask us for more details.

Have you used Mouse FCRL1 ELISA Kit PicoKine®?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Mouse FCRL1 ELISA Kit PicoKine®

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives product credits. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

7 Customer Q&As for Mouse FCRL1 ELISA Kit PicoKine®

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

K. Tiwari

Verified customer

Asked: 2020-12-21

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-12-21

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement FCRLA using the EK1977 Mouse FCRLA Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2020-10-08

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample right after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2020-10-08

Question

Q: What is the optimal O.D. value for FCRLA ELISA kit? I used your FCRLA ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain FCRLA even though the O.D. values are not very high?

A. Hill

Verified customer

Asked: 2020-04-28

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. in the example mentioned, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this FCRLA ELISA kit to have sensitivity of 50pg/ml, that means the minimum amount of FCRLA that can be declared/interpreted as positive by the above standard is 50pg/ml.

Boster Scientific Support

Answered: 2020-04-28

Question

Q: if the enzyme conjugated FCRLA antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the FCRLA antigen?

K. Kaur

Verified customer

Asked: 2019-01-08

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-01-08

Question

Q: how to analyze ELISA data? I have obtained FCRLA level in plasma.

Verified Customer

Verified customer

Asked: 2017-08-03

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2017-08-03

Question

Q: how can I thaw whole blood sample for FCRLA ELISA after freezing?

Verified Customer

Verified customer

Asked: 2016-04-01

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test FCRLA for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2016-04-01

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

G. Lopez

Verified customer

Asked: 2014-06-05

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2014-06-05

$369

Ask a question

Get A Quote

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

Product Categories

Primary Antibodies

Primary and Secondary Antibodies

Boster Bio offers over 16,000 antibodies that have been validated for IHC, WB, ELISA, and FC in human, mouse, and rat samples. Rabbit and mouse monoclonal antibodies as well as rabbit polyclonal antibodies are available. Buy a primary antibody and get a secondary antibody for free!

ELISA Kits

ELISA Kits

More than 1,000 ELISA kits, both singleplex and multiplex, that have been cited 6,000+ times are available at Boster. We offer our Boster-branded Picokine™ ELISA kits (sandwich ELISA) and EZ-Set™ ELISA kits (DIY antibody pairs) in addition to many multiplex ELISA kits.

Image

Recombinant Proteins

Boster provides a wide range of human, mouse, and rat recombinant proteins, which include cytokines, growth factors, chemokines, and many more. Our recombinant proteins have high stability, biological activity, and purity.

Boster Kit

Reagents

Boster offers all the reagents you need for your immunostaining and western blotting experiments. We've got everything from buffers to lysates to kits, including our popular and well-cited BCA, CCK-8, and MTT assay kits.

In stock
Order Product
EK1977
$369.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.