Mouse Fibronectin ELISA Kit PicoKine™

Mouse Fibronectin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Fn1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml. Cited in 11 publication(s).

Product Info Summary

SKU: EK0351
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Product Name

Mouse Fibronectin ELISA Kit PicoKine™

See all Fibronectin products

SKU/Catalog Number

EK0351

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Fibronectin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Fn1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse Fibronectin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0351)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: from plasma

Sensitivity

<15 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0351 is reactive to Fn1 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK0351 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0351-CAP Anti-Mouse Fn1 Pre-coated 96-well strip microplate 1
EK0351-ST Mouse Fn1 Standard 2 vials, 10 ng/tube
EK0351-DA Mouse Fn1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0480.0850.1790.2640.3660.7411.2581.990

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Fibronectin, Dilution ratio of 1:400000, concentration in serum is around 500ug.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Fibronectin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2061683432321918433947
Standard deviation13.39126.22293.9615.54160.34335.49
CV (%)6.5%7.5%6.8%7.1%8.7%8.5%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Fibronectin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 120622923521522111.45.1%
Sample 21683159717491766169866.423.9%
Sample 343234412479640124385279.566.3%
*number of samples for each test n=16.

Gene/Protein Information For Fn1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P11276

Gene ID

14268

Gene Name

Fn1

Full Name

Fibronectin

Weight

272.538kDa

Alternative Names

CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF Fn1|E330027I09, F, Fn, Fn-, Fn-1|fibronectin 1|fibronectin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Fn1, check out the Fn1 Infographic

Fn1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Fn1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0351 has been cited in 11 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

A Chinese Herbal Formula Ameliorates Pulmonary Fibrosis by Inhibiting Oxidative Stress via Upregulating Nrf2

Damage-associated molecular patterns (DAMPs) released after burn are associated with inflammation and monocyte activation

Zhao X, Xu L, Sun M, Ma W, Wu X, Xu C, Kuang H. Nat Commun. 2017 Dec 8;8(1):2007. doi: 10.1038/s41467-017-02268-8. Tuning the interactions between chiral plasmonic films and living cells

Lv C, Wu C, Zhou Yh, Shao Y, Wang G, Wang Qy. Int J Endocrinol. 2014;2014:658589. Doi: 10.1155/2014/658589. Epub 2014 Oct 30. Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction Via Mtor/P70S6K/4E-Bp1 Pathway.

Gao L, Huang W, Li J. Mol Cell Biochem. 2013 Oct;382(1-2):185-91. Doi: 10.1007/S11010-013-1733-4. Epub 2013 Jun 26. Nox1 Abet Mesangial Fibrogenesis Via Inos Induction In Diabetes.

Mao T, Gao L, Li H, Li J. Saudi Med J. 2011 Aug;32(8):769-77. Pigment Epithelium-Derived Factor Inhibits High Glucose Induced Oxidative Stress And Fibrosis Of Cultured Human Glomerular Mesangial Cells.

Yang L, Cheng F, Liu T, Lu Jr, Song K, Jiang L, Wu S, Guo W. Biomed Mater. 2012 Jun;7(3):035003. Doi: 10.1088/1748-6041/7/3/035003. Epub 2012 Feb 23. Comparison Of Mesenchymal Stem Cells Released From Poly(N-Isopropylacrylamide) Copolymer Film And...

Tang Kf, Song Gb, Shi Ys, Yuan L, Li Yh. Biochim Biophys Acta. 2010 Mar;1800(3):380-4. Doi: 10.1016/J.Bbagen.2009.11.009. Epub 2009 Nov 13. Dicer Knockdown Induces Fibronectin-1 Expression In Hek293T Cells Via Induction Of Egr1.

Zeng R, Han M, Luo Y, Li C, Pei G, Liao W, Bai S, Ge S, Liu X, Xu G. Nephrol Dial Transplant. 2011 Apr;26(4):1149-56. Doi: 10.1093/Ndt/Gfq619. Epub 2010 Oct 19. Role Of Sema4C In Tgf-??1-Induced Mitogen-Activated Protein Kinase Activation And Epit...

Bai S, Zeng R, Zhou Q, Liao W, Zhang Y, Xu C, Han M, Pei G, Liu L, Liu X, Yao Y, Xu G. Int J Biol Sci. 2012;8(6):859-69. Doi: 10.7150/Ijbs.3490. Epub 2012 Jun 13. Cdc42-Interacting Protein-4 Promotes Tgf-??1-Induced Epithelial-Mesenchymal Transiti...

Xu Ww, Guan Mp, Zheng Zj, Gao F, Zeng Ym, Qin Y, Xue Ym. Cell Physiol Biochem. 2014;33(2):423-32. Doi: 10.1159/000358623. Epub 2014 Feb 11. Exendin-4 Alleviates High Glucose-Induced Rat Mesangial Cell Dysfunction Through The Ampk Pathway.

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12 Customer Q&As for Mouse Fibronectin ELISA Kit PicoKine™

Question

Q: Can Fibronectin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2020-04-22

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2020-04-22

Question

Q: can I use citrate plasma as samples in Mouse Fibronectin Picokine® ELISA Kit (Catalog # EK0351)?

Verified Customer

Verified customer

Asked: 2020-02-26

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Fibronectin causing disruption of its protein structure. Fibronectin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Fibronectin ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-02-26

Question

Q: how do I analyze ELISA data? I measured Fibronectin level in plasma.

Verified Customer

Verified customer

Asked: 2019-08-01

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-08-01

Question

What is the immunogen for the capture antibody for EK0351?

Verified customer

Asked: 2019-05-15

Answer

For the Mouse Fibronectin ELISA Kit PicoKine™ (EK0351), the immunogen is natural protein from plasma.

Boster Scientific Support

Answered: 2019-05-15

Question

What are tested samples for EK0351?

Verified customer

Asked: 2019-05-15

Answer

The tested samples for the Mouse Fibronectin ELISA Kit PicoKine™ (EK0351) are mouse cell culture supernates, serum and plasma (heparin, EDTA, citrate).

Boster Scientific Support

Answered: 2019-05-15

Question

What is the standard source protein for EK0351?

Verified customer

Asked: 2019-05-15

Answer

The standard source protein for the Mouse Fibronectin ELISA Kit PicoKine™ (EK0351) is Natural protein from plasma.

Boster Scientific Support

Answered: 2019-05-15

Question

Q: if the enzyme conjugated Fibronectin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Fibronectin antigen?

M. Mitchell

Verified customer

Asked: 2019-04-25

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-04-25

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-03-26

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-03-26

Question

What type of fibronectin does EK0351 detect - cellular fibronectin, plasma fibronectin, or both? <br> Keywords: detection, fibronectin type

Verified Customer

Verified customer

Asked: 2019-02-12

Answer

EK0351 detects both types of fibronectin, cellular and plasma.

Boster Scientific Support

Answered: 2019-02-12

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-01-31

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-01-31

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Fibronectin using the EK0351 Mouse Fibronectin Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-03-02

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2017-03-02

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

S. Puri

Verified customer

Asked: 2014-03-13

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2014-03-13

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