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Product Info Summary
| SKU: | EK0351 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse Fibronectin ELISA Kit PicoKine®
SKU/Catalog Number
EK0351
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Fibronectin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Fn1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Fibronectin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0351)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Target protein isolated from mouse plasma
Capture/Detection Antibodies
The capture antibody is polyclonal antibody from rabbit and the detection antibody is polyclonal antibody from rabbit.
Sensitivity
<15 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0351 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0351 is reactive to Fn1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
EK0351 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Fn1
Fibronectin (FN) also known as LETS, is identified on the surfFN of fibroblasts by labeling with radioactive compounds or specific antibodies. Fibronectin is a 430,000-dalton dimeric glycoprotein that exists in 2 forms, termed cellular and plasma fibronectin. Cellular and plasma fibronectins are heterodimers consisting of similar but not identical polypeptides. These two forms of FN differ in biologic activity. Fibronectins bind cell surfFNs and various compounds including collagen, fibrin, heparin, DNA, and actin. Because fibronectin stimulates endocytosis in several systems and promotes the clearance of particulate material from the circulation, it could function in the clearance of C1q-coated material such as immune complexes or cellular debris. Fibronectins are involved in cell adhesion, cell motility, opsonization, would healing, and maintenance of cell shape. LETS, encoded on chromosome 8, is responsible for the LETS protein expression in humans. Because LETS has been implicated in tumorigenicity and cellular transformation, it is of interest that rearrangement or modifications in the number of chromosome 8 have been associated with certain forms of cancer.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0351-CAP | Anti-Mouse Fn1 Pre-coated 96-well strip microplate | 1 |
| EK0351-ST | Mouse Fn1 Standard | 2 vials, 10 ng/tube |
| EK0351-DA | Mouse Fn1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.034 | 0.092 | 0.14 | 0.22 | 0.397 | 0.691 | 1.251 | 1.91 |
Data Example Images
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Mouse Fibronectin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Fn1, Dilution ratio of 1:400000, concentration in serum and plasma is around 500 ug/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Fibronectin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 206 | 1683 | 4323 | 219 | 1843 | 3947 |
| Standard deviation | 13.39 | 126.22 | 293.96 | 15.54 | 160.34 | 335.49 |
| CV (%) | 6.5% | 7.5% | 6.8% | 7.1% | 8.7% | 8.5% |
Specific Publications For Mouse Fibronectin ELISA Kit PicoKine® (EK0351)
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Customer Reviews
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Customer Q&As
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12 Customer Q&As for Mouse Fibronectin ELISA Kit PicoKine®
Question
Q: Can Fibronectin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2020-04-22
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please view the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2020-04-22
Question
Q: can I use citrate plasma as samples in Mouse Fibronectin Picokine® ELISA Kit (Catalog # EK0351)?
Verified Customer
Verified customer
Asked: 2020-02-26
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can irreversibly bind metal ions from the functional domain of Fibronectin causing disruption of its protein structure. Fibronectin may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the Fibronectin ELISA, treating samples with various anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-02-26
Question
Q: how do I analyze ELISA data? I measured Fibronectin level in plasma.
Verified Customer
Verified customer
Asked: 2019-08-01
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2019-08-01
Question
What is the immunogen for the capture antibody for EK0351?
Verified customer
Asked: 2019-05-15
Answer
For the Mouse Fibronectin ELISA Kit PicoKine™ (EK0351), the immunogen is natural protein from plasma.
Boster Scientific Support
Answered: 2019-05-15
Question
What are tested samples for EK0351?
Verified customer
Asked: 2019-05-15
Answer
The tested samples for the Mouse Fibronectin ELISA Kit PicoKine™ (EK0351) are mouse cell culture supernates, serum and plasma (heparin, EDTA, citrate).
Boster Scientific Support
Answered: 2019-05-15
Question
What is the standard source protein for EK0351?
Verified customer
Asked: 2019-05-15
Answer
The standard source protein for the Mouse Fibronectin ELISA Kit PicoKine™ (EK0351) is Natural protein from plasma.
Boster Scientific Support
Answered: 2019-05-15
Question
Q: if the enzyme conjugated Fibronectin antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Fibronectin antigen?
M. Mitchell
Verified customer
Asked: 2019-04-25
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-04-25
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2019-03-26
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2019-03-26
Question
What type of fibronectin does EK0351 detect - cellular fibronectin, plasma fibronectin, or both? <br> Keywords: detection, fibronectin type
Verified Customer
Verified customer
Asked: 2019-02-12
Answer
EK0351 detects both types of fibronectin, cellular and plasma.
Boster Scientific Support
Answered: 2019-02-12
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-01-31
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-01-31
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Fibronectin using the EK0351 Mouse Fibronectin Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-03-02
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2017-03-02
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
S. Puri
Verified customer
Asked: 2014-03-13
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2014-03-13
