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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
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Mouse Fibronectin ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse Fibronectin ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Fn1 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 15pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse Fibronectin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0351)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: from plasma
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0351 is reactive to Fn1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
EK0351 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0351-CAP||Anti-Mouse Fn1 Pre-coated 96-well strip microplate||1|
|EK0351-ST||Mouse Fn1 Standard||2 vials, 10 ng/tube|
|EK0351-DA||Mouse Fn1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse Fibronectin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Fibronectin, Dilution ratio of 1:400000, concentration in serum is around 500ug.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Fibronectin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Fibronectin in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Fn1 (Source: Uniprot.Org, NCBI)
CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF Fn1|E330027I09, F, Fn, Fn-, Fn-1|fibronectin 1|fibronectin*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Fn1, check out the Fn1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Fn1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0351 has been cited in 11 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
A Chinese Herbal Formula Ameliorates Pulmonary Fibrosis by Inhibiting Oxidative Stress via Upregulating Nrf2
Damage-associated molecular patterns (DAMPs) released after burn are associated with inflammation and monocyte activation
Zhao X, Xu L, Sun M, Ma W, Wu X, Xu C, Kuang H. Nat Commun. 2017 Dec 8;8(1):2007. doi: 10.1038/s41467-017-02268-8. Tuning the interactions between chiral plasmonic films and living cells
Lv C, Wu C, Zhou Yh, Shao Y, Wang G, Wang Qy. Int J Endocrinol. 2014;2014:658589. Doi: 10.1155/2014/658589. Epub 2014 Oct 30. Alpha Lipoic Acid Modulated High Glucose-Induced Rat Mesangial Cell Dysfunction Via Mtor/P70S6K/4E-Bp1 Pathway.
Gao L, Huang W, Li J. Mol Cell Biochem. 2013 Oct;382(1-2):185-91. Doi: 10.1007/S11010-013-1733-4. Epub 2013 Jun 26. Nox1 Abet Mesangial Fibrogenesis Via Inos Induction In Diabetes.
Mao T, Gao L, Li H, Li J. Saudi Med J. 2011 Aug;32(8):769-77. Pigment Epithelium-Derived Factor Inhibits High Glucose Induced Oxidative Stress And Fibrosis Of Cultured Human Glomerular Mesangial Cells.
Yang L, Cheng F, Liu T, Lu Jr, Song K, Jiang L, Wu S, Guo W. Biomed Mater. 2012 Jun;7(3):035003. Doi: 10.1088/1748-6041/7/3/035003. Epub 2012 Feb 23. Comparison Of Mesenchymal Stem Cells Released From Poly(N-Isopropylacrylamide) Copolymer Film And...
Tang Kf, Song Gb, Shi Ys, Yuan L, Li Yh. Biochim Biophys Acta. 2010 Mar;1800(3):380-4. Doi: 10.1016/J.Bbagen.2009.11.009. Epub 2009 Nov 13. Dicer Knockdown Induces Fibronectin-1 Expression In Hek293T Cells Via Induction Of Egr1.
Zeng R, Han M, Luo Y, Li C, Pei G, Liao W, Bai S, Ge S, Liu X, Xu G. Nephrol Dial Transplant. 2011 Apr;26(4):1149-56. Doi: 10.1093/Ndt/Gfq619. Epub 2010 Oct 19. Role Of Sema4C In Tgf-??1-Induced Mitogen-Activated Protein Kinase Activation And Epit...
Bai S, Zeng R, Zhou Q, Liao W, Zhang Y, Xu C, Han M, Pei G, Liu L, Liu X, Yao Y, Xu G. Int J Biol Sci. 2012;8(6):859-69. Doi: 10.7150/Ijbs.3490. Epub 2012 Jun 13. Cdc42-Interacting Protein-4 Promotes Tgf-??1-Induced Epithelial-Mesenchymal Transiti...
Xu Ww, Guan Mp, Zheng Zj, Gao F, Zeng Ym, Qin Y, Xue Ym. Cell Physiol Biochem. 2014;33(2):423-32. Doi: 10.1159/000358623. Epub 2014 Feb 11. Exendin-4 Alleviates High Glucose-Induced Rat Mesangial Cell Dysfunction Through The Ampk Pathway.
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