Mouse IFNAR1 ELISA Kit PicoKine®

IFN-alpha/beta R1 ELISA kit for Mouse

Mouse IFNAR1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse IFNAR1 in cell culture supernatants, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml. Cited in 3 publication(s).

Product Info Summary

SKU: EK1991
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin or EDTA)

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Product Name

Mouse IFNAR1 ELISA Kit PicoKine®

View all IFN-alpha/beta R1 ELISA kits

SKU/Catalog Number

EK1991

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IFNAR1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse IFNAR1 in cell culture supernatants, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse IFNAR1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1991)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E27-T429

Sensitivity

<10 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1991 is reactive to IFNAR1 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin or EDTA)

Application Guarantee

EK1991 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IFN-alpha/beta R1

Interferon-alpha/beta receptor alpha chain is a protein that in humans is encoded by the IFNAR1 gene. It is mapped to 16 C3.3; 16 52.98 cM. The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor.

Kit Components

Catalog Number Description Quantity
EK1991-CAP Anti-Mouse IFNAR1 Pre-coated 96-well strip microplate 1
EK1991-ST Mouse IFNAR1 Standard 2 vials, 10 ng/tube
EK1991-DA Mouse IFNAR1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive product credits you can use towards any Boster products! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0350.0800.1240.3070.3980.8511.6262.067

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IFN-alpha/beta R1, Dilution ratio of 1:1, concentration in serum is around 1000pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IFNAR1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)12348918221244921897
Standard deviation5.0422.9872.887.0726.08121.41
CV (%)4.1%4.7%4.0%5.7%5.3%6.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IFN-alpha/beta R1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11231121141051136.425.6%
Sample 248941149848947135.267.4%
Sample 31822195817751754182779.404.3%
*number of samples for each test n=16.

Gene/Protein Information For IFNAR1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P33896

Gene ID

15975

Gene Name

IFNAR1

Full Name

Interferon alpha/beta receptor 1

Weight

65.796kDa

Superfamily

type II cytokine receptor family

Alternative Names

alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta R1; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-bR1; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1 Ifnar1|I, If, Ifar, Ifnar, Ifrc, Infar|interferon (alpha and beta) receptor 1|interferon alpha/beta receptor 1|IFN-R-1|IFN-alpha/beta receptor 1|IFN-alpha/betaR|INF-a receptor|type I interferon receptor 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on IFNAR1, check out the IFNAR1 Infographic

IFNAR1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for IFNAR1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1991 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Vinpocetine Inhibited the CpG Oligodeoxynucleotide-induced Immune Response in Plasmacytoid Dendritic Cells

Coxsackievirus B3 induces the formation of autophagosomes in cardiac fibroblasts both in vitro and in vivo

WEI Wen-Long,WU Shi-Fei,LI Hao-Jv,LI Zhen-Wei,QU Hua,YAO Chang-Liang,ZHANG Jian-Qing,LI Jia-Yuan,WU Wan-Ying , GUO De-An.Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS [J].Chin J Nat Med,2021,19(6):473-480.doi:10.1016/S1875-5364(21)60046-8
Species: Mouse
EK1991 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA

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7 Customer Q&As for Mouse IFNAR1 ELISA Kit PicoKine®

Question

Q: how to analyze ELISA data? I have performed IFN-alpha level in serum.

Verified Customer

Verified customer

Asked: 2020-11-05

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-11-05

Question

Q: if the enzyme conjugated IFN-alpha antibodies are mixed with the substrate, will that change the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IFN-alpha antigen?

Verified Customer

Verified customer

Asked: 2020-08-24

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-08-24

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

T. Mitchell

Verified customer

Asked: 2019-12-16

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-12-16

Question

Q: Can IFN-alpha ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

G. Chen

Verified customer

Asked: 2019-10-10

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-10-10

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

J. Varma

Verified customer

Asked: 2019-08-21

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-08-21

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-07-10

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-07-10

Question

Q: which procedure should I follow in order to thaw whole blood sample for IFN-alpha ELISA after freezing?

Verified Customer

Verified customer

Asked: 2017-12-14

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IFN-alpha for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2017-12-14

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