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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin or EDTA)|
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Mouse IFNAR1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse IFNAR1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse IFNAR1 in cell culture supernatants, serum and plasma (heparin or EDTA) . Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IFNAR1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1991)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: E27-T429
62.5 pg/ml - 4,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1991 is reactive to IFNAR1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin or EDTA)
EK1991 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IFN-alpha/beta R1
Interferon-alpha/beta receptor alpha chain is a protein that in humans is encoded by the IFNAR1 gene. It is mapped to 16 C3.3; 16 52.98 cM. The protein encoded by this gene is a type I membrane protein that forms one of the two chains of a receptor for interferons alpha and beta. Binding and activation of the receptor stimulates Janus protein kinases, which in turn phosphorylate several proteins, including STAT1 and STAT2. The encoded protein also functions as an antiviral factor.
|EK1991-CAP||Anti-Mouse IFNAR1 Pre-coated 96-well strip microplate||1|
|EK1991-ST||Mouse IFNAR1 Standard||2 vials, 10 ng/tube|
|EK1991-DA||Mouse IFNAR1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse IFNAR1 PicoKine ELISA Kit Standard Curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IFN-alpha/beta R1, Dilution ratio of 1:1, concentration in serum is around 1000pg/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IFNAR1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of IFN-alpha/beta R1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For IFNAR1 (Source: Uniprot.Org, NCBI)
Interferon alpha/beta receptor 1
type II cytokine receptor family
alpha-type antiviral protein; AVP; beta-type antiviral protein; CRF2-1; Cytokine receptor class-II member 1; Cytokine receptor family 2 member 1; human interferon-alpha receptor (HuIFN-alpha-Rec)10IFRC; IFN-alpha/beta R1; IFN-alpha/beta receptor 1; IFN-alpha-REC; IFNAR; IFNAR1; IFN-aR1; IFNBR; IFNbR1; IFN-bR1; IFN-R-1; interferon (alpha, beta and omega) receptor 1; interferon alpha/beta receptor 1; interferon-alpha/beta receptor alpha chain; interferon-beta receptor 1; Type I interferon receptor 1 Ifnar1|I, If, Ifar, Ifnar, Ifrc, Infar|interferon (alpha and beta) receptor 1|interferon alpha/beta receptor 1|IFN-R-1|IFN-alpha/beta receptor 1|IFN-alpha/betaR|INF-a receptor|type I interferon receptor 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on IFNAR1, check out the IFNAR1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for IFNAR1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK1991 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Vinpocetine Inhibited the CpG Oligodeoxynucleotide-induced Immune Response in Plasmacytoid Dendritic Cells
Coxsackievirus B3 induces the formation of autophagosomes in cardiac fibroblasts both in vitro and in vivo
WEI Wen-Long,WU Shi-Fei,LI Hao-Jv,LI Zhen-Wei,QU Hua,YAO Chang-Liang,ZHANG Jian-Qing,LI Jia-Yuan,WU Wan-Ying , GUO De-An.Chemical profiling of Huashi Baidu prescription, an effective anti-COVID-19 TCM formula, by UPLC-Q-TOF/MS [J].Chin J Nat Med,2021,19(6):473-480.doi:10.1016/S1875-5364(21)60046-8
EK1991 usage in article: APP:ELISA, SAMPLE:RAW264.7 CELL, DILUTION:NA
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