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Product Info Summary
| SKU: | EK2285 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum (heparin, EDTA). |
Product info
Product Name
Mouse IFN-Beta ELISA Kit PicoKine®
SKU/Catalog Number
EK2285
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IFN-Beta ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Ifnb1 in cell culture supernatants, serum (heparin, EDTA). Sensitivity: 2 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4℃ for 6 months, at -20℃ for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IFN-Beta ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2285)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: HEK293; Immunogen sequence: I22-N182
Capture/Detection Antibodies
The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Standard Dilution Instructions
See datasheet of EK2285 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK2285 is reactive to Ifnb1 in Mouse samples
Validated Sample Types
cell culture supernatants, serum (heparin, EDTA).
Application Guarantee
EK2285 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Ifnb1
Interferon beta is a protein that in humans is encoded by the IFNB1 gene. This gene encodes a cytokine that belongs to the interferon family of signaling proteins, which are released as part of the innate immune response to pathogens. The protein encoded by this gene belongs to the type I class of interferons, which are important for defense against viral infections. In addition, type I interferons are involved in cell differentiation and anti-tumor defenses. Following secretion in response to a pathogen, type I interferons bind a homologous receptor complex and induce transcription of genes such as those encoding inflammatory cytokines and chemokines. Overactivation of type I interferon secretion is linked to autoimmune diseases. Mice deficient for this gene display several phenotypes including defects in B cell maturation and increased susceptibility to viral infection.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK2285-CAP | Anti-Mouse Ifnb1 Pre-coated 96-well strip microplate | 1 |
| EK2285-ST | Mouse Ifnb1 Standard | 2 vials, 4 ng/tube |
| EK2285-DA | Mouse Ifnb1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.008 | 0.1 | 0.17 | 0.297 | 0.537 | 0.97 | 1.806 | 2.443 |
Data Example Images
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Mouse IFN-Beta ELISA Kit PicoKine standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Ifnb1, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 62.5 pg/ml. .
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IFN-Beta ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 72 | 95 | 107 | 70 | 86 | 3.76 | 4.4% |
| Sample 2 | 354 | 365 | 401 | 288 | 352 | 14.89 | 4.2% |
| Sample 3 | 1168 | 1375 | 1157 | 1831 | 1383 | 74.53 | 5.4% |
Specific Publications For Mouse IFN-Beta ELISA Kit PicoKine® (EK2285)
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