Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine™

Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il1rl1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1180
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine™

See all ST2/IL-33R products

SKU/Catalog Number

EK1180

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Il1rl1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1180)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S27-R332

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1180 is reactive to Il1rl1 in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1180 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1180-CAP Anti-Mouse Il1rl1 Pre-coated 96-well strip microplate 1
EK1180-ST Mouse Il1rl1 Standard 2 vials, 10 ng/tube
EK1180-DA Mouse Il1rl1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512500025000500010000
O.D0.0200.1490.1490.2800.4400.9321.6292.353

Data Example Images

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)1941525548418915385883
Standard deviation8.7394.55296.1310.77115.35394.16
CV (%)4.5%6.2%5.4%5.7%7.5%6.7%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of ST2/IL-33R in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11941921992061975.42.7%
Sample 21525136615021386144469.584.8%
Sample 354845267484252025198230.924.4%
*number of samples for each test n=16.

Gene/Protein Information For Il1rl1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P14719

Gene ID

17082

Gene Name

Il1rl1

Full Name

Interleukin-1 receptor-like 1

Weight

64.801kDa

Superfamily

interleukin-1 receptor family

Alternative Names

DER4; DER4ST2growth stimulation-expressed; Fit-1; IL-1 R4; IL1R4; IL-1R4; IL-1RL1; IL-33 R; IL33R; IL-33R; interleukin 1 receptor-like 1; interleukin 1 receptor-related protein; interleukin-1 receptor-like 1; Ly84; MGC32623; Protein ST2; ST2; ST2L; ST2V; T1; T1homolog of mouse growth stimulation-expressed Il1rl1|DER, DER4, Fit-, Fit-1, Ly8, Ly84, ST, ST2L, St2, St2-, St2-rs1, T1, T1/ST, T1/ST2|interleukin 1 receptor-like 1|interleukin-1 receptor-like 1|interleukin-33 receptor alpha chain|lymphocyte antigen 84

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il1rl1, check out the Il1rl1 Infographic

Il1rl1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il1rl1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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8 Customer Q&As for Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine™

Question

Q: is it okay to use citrate plasma as samples in Mouse ST2 Picokine® ELISA Kit (Catalog # EK1180)?

N. Bhatt

Verified customer

Asked: 2020-08-10

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of ST2 causing disruption of its protein structure. ST2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the ST2 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-08-10

Question

What is the immogen sequence of the the antibody pair used for EK1180?

Verified customer

Asked: 2019-07-01

Answer

For the Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine™ (EK1180), the sequence of the standard for both the capture and detection antibody is S27-R332.

Boster Scientific Support

Answered: 2019-07-01

Question

Q: can you recommend the dilution ratio of serum samples for detection of ST2 in Mouse cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2019-03-10

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the ST2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-03-10

Question

Q: how to analyze ELISA data? I measured ST2 level in cell lysates.

H. Bhasin

Verified customer

Asked: 2018-10-25

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-10-25

Question

Q: What is the optimal O.D. value for ST2 ELISA kit? I used your ST2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain ST2 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-09-24

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ST2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of ST2 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-09-24

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-04-06

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-04-06

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

M. Garcia

Verified customer

Asked: 2018-03-02

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2018-03-02

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

L. Jha

Verified customer

Asked: 2017-11-11

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-11-11

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