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Product Info Summary
| SKU: | EK1180 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine®
SKU/Catalog Number
EK1180
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il1rl1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1180)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S27-R332
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK1180 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1180 is reactive to Il1rl1 in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1180 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Il1rl1
Interleukin 1 receptor-like 1, also known as IL1RL1 or ST2, is a protein that in humans is encoded by the IL1RL1 gene. The protein encoded by this gene is a member of the interleukin 1 receptor family. Studies of the similar gene in mouse suggested that this receptor can be induced by proinflammatory stimuli, and may be involved in the function of helper T cells. TL1RL1 is necessary for endotoxin tolerance and, by inhibiting TLR responses, enhances Th2 responses. This gene, interleukin 1 receptor, type I (IL1R1), interleukin 1 receptor, type II (IL1R2) and interleukin 1 receptor-like 2 (IL1RL2) form a cytokine receptor gene cluster in a region mapped to chromosome 2q12.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1180-CAP | Anti-Mouse Il1rl1 Pre-coated 96-well strip microplate | 1 |
| EK1180-ST | Mouse Il1rl1 Standard | 2 vials, 10 ng/tube |
| EK1180-DA | Mouse Il1rl1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.033 | 0.04 | 0.052 | 0.093 | 0.172 | 0.409 | 1.028 | 2.108 |
Data Example Images
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Mouse IL1RL1/ST2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Il1rl1, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 156 pg/ml..
Some articles we found to cite concentrations of Il1rl1 in samples: 22542450 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 194 | 1525 | 5484 | 189 | 1538 | 5883 |
| Standard deviation | 8.73 | 94.55 | 296.13 | 10.77 | 115.35 | 394.16 |
| CV (%) | 4.5% | 6.2% | 5.4% | 5.7% | 7.5% | 6.7% |
Specific Publications For Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine® (EK1180)
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Customer Reviews
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Customer Q&As
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8 Customer Q&As for Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine®
Question
What is the dilution factor for EK1180?
Verified customer
Asked: 2022-03-17
Answer
The recommended dilution factor for mouse serum samples assayed with Mouse IL-1 RL1 / ST2 ELISA Kit PicoKine® (EK1180) is 1:10.
Boster Scientific Support
Answered: 2022-03-18
Question
Q: is it okay to use citrate plasma as samples in Mouse ST2 Picokine® ELISA Kit (Catalog # EK1180)?
N. Bhatt
Verified customer
Asked: 2020-08-10
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of ST2 causing disruption of its protein structure. ST2 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the ST2 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-08-10
Question
Q: can you recommend the dilution ratio of serum samples for detection of ST2 in Mouse cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2019-03-10
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the ST2 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/elisa-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2019-03-10
Question
Q: how to analyze ELISA data? I measured ST2 level in cell lysates.
H. Bhasin
Verified customer
Asked: 2018-10-25
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2018-10-25
Question
Q: What is the optimal O.D. value for ST2 ELISA kit? I used your ST2 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain ST2 even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2018-09-24
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. a point of focus should be is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. typically, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this ST2 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of ST2 that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2018-09-24
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2018-04-06
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can have an affect on immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2018-04-06
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
M. Garcia
Verified customer
Asked: 2018-03-02
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-03-02
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
L. Jha
Verified customer
Asked: 2017-11-11
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2017-11-11
