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Product Info Summary
| SKU: | EK1228 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine®
SKU/Catalog Number
EK1228
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse F11r in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1228)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: K27-G238
Sensitivity
<10 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1228 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1228 is reactive to F11r in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1228 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of JAM-A
Junctional adhesion molecule A (JAM-A) is a protein that in humans is encoded by the F11R gene. It is mapped to 1q23.3. This gene is an immunoglobulin-like molecule that colocalizes with tight junctions in endothelium and epithelium and is also found on blood leukocytes and platelets. JAM-A plays an important role in the regulation of tight junction assembly in epithelia. In addition, it can act as a receptor for reovirus, a ligand for the integrin LFA1, involved in leukocyte transmigration, and a platelet receptor. JAM-A has a nonredundant role in controlling DC motility, trafficking to lymph nodes, and activation of specific immunity.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK1228-CAP | Anti-Mouse F11r Pre-coated 96-well strip microplate | 1 |
| EK1228-ST | Mouse F11r Standard | 2 vials, 10 ng/tube |
| EK1228-DA | Mouse F11r Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.006 | 0.137 | 0.214 | 0.375 | 0.722 | 1.097 | 1.734 | 2.209 |
Data Example Images
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Mouse JAM-A/F11R PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect JAM-A, Dilution ratio of 1:1, concentration in serum and plasma is around 250 pg/ml..
Some articles we found to cite concentrations of JAM-A in samples: 19258599 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 74 | 243 | 871 | 67 | 225 | 792 |
| Standard deviation | 3.18 | 18.95 | 57.48 | 3.28 | 18.67 | 61.77 |
| CV (%) | 4.3% | 7.8% | 6.6% | 4.9% | 8.3% | 7.8% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of JAM-A in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 74 | 76 | 83 | 81 | 78 | 3.64 | 4.6% |
| Sample 2 | 243 | 243 | 259 | 220 | 241 | 13.89 | 5.7% |
| Sample 3 | 871 | 854 | 977 | 972 | 918 | 56.34 | 6.1% |
Protein Target Info & Infographic
Gene/Protein Information For F11r (Source: Uniprot.Org, NCBI)
Gene Name
F11r
Full Name
Junctional adhesion molecule A
Weight
32.424kDa
Superfamily
immunoglobulin superfamily
Alternative Names
CD321 antigen; CD321; F11 receptor; F11R; JAM-1; JAM1CD321; JAMA; JAM-A; JCAMJAM; Junctional adhesion molecule 1Platelet F11 receptor; junctional adhesion molecule A; PAM-1; PAM-1KAT; Platelet adhesion molecule 1 F11r|9130004G24, AA638916, ESTM3, ESTM33, JAM, JAM-, JAM-1, JAM-A, Jcam, Jcam1, Ly10, Ly106|F11 receptor|junctional adhesion molecule A|BV11 antigen|F11 receptor/F11R|junction cell adhesion molecule A|junction cell adhesion molecule1|junctional adhesion molecule 1
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on F11r, check out the F11r Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for F11r: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine® (EK1228)
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No publications found for EK1228
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine®
Question
Q: can you suggest the dilution ratio of serum samples for detection of JAM-A in Mouse cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.
W. Chen
Verified customer
Asked: 2020-11-22
Answer
A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the JAM-A ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2020-11-22
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
M. Scott
Verified customer
Asked: 2019-08-07
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2019-08-07
Question
What are the normal levels of F11R seen in Naive animals (Murine) using EK1228?
Verified customer
Asked: 2019-04-18
Answer
For the Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ (EK1228), the normal levels of F11R seen in healthy mouse serum and plasma is 200-300pg/ml.
Boster Scientific Support
Answered: 2019-04-23
Question
What is the suggested dilution for serum and plasma samples with EK1228?
Verified customer
Asked: 2019-04-18
Answer
The suggested dilution for serum and plasma samples using the Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ (EK1228) is 1:1.
Boster Scientific Support
Answered: 2019-04-23
Question
Q: Can JAM-A ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
Verified Customer
Verified customer
Asked: 2019-01-12
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2019-01-12
Question
Q: is it okay to use citrate plasma as samples in Mouse JAM-A Picokine® ELISA Kit (Catalog # EK1228)?
Verified Customer
Verified customer
Asked: 2018-08-30
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of JAM-A causing degradation of its protein structure. JAM-A may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the JAM-A ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2018-08-30
Question
Q: how do I analyze ELISA data? I have obtained JAM-A level in cell lysates.
Verified Customer
Verified customer
Asked: 2018-04-25
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-04-25
Question
I am planning on running the JAML-A/F11R ELISA in samples from animals in various models of murine IBD. What are the normal levels seen in Naive animals and what dilution do you suggest I start with?
Verified Customer
Verified customer
Asked: 2018-03-13
Answer
The normal levels of F11R seen in healthy mouse serum and plasma is
Boster Scientific Support
Answered: 2018-03-13
Question
Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?
B. Singh
Verified customer
Asked: 2016-07-30
Answer
A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2016-07-30
Question
Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring JAM-A using the EK1228 Mouse JAM-A Picokine® ELISA Kit?
W. Zhou
Verified customer
Asked: 2016-02-17
Answer
A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.
Boster Scientific Support
Answered: 2016-02-17
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
V. Anand
Verified customer
Asked: 2015-12-19
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2015-12-19
