Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™

Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse F11r in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1228
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™

See all JAM-A products

SKU/Catalog Number

EK1228

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse F11r in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1228)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: K27-G238

Sensitivity

<10 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1228 is reactive to F11r in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1228 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1228-CAP Anti-Mouse F11r Pre-coated 96-well strip microplate 1
EK1228-ST Mouse F11r Standard 2 vials, 10 ng/tube
EK1228-DA Mouse F11r Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0060.1370.2140.3750.7221.0971.7342.209

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect JAM-A, Dilution ratio of 1:1, concentration in serum is around 250pg/ml.

Some articles we found to cite concentrations of JAM-A in samples: 19258599 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)7424387167225792
Standard deviation3.1818.9557.483.2818.6761.77
CV (%)4.3%7.8%6.6%4.9%8.3%7.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of JAM-A in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 174768381783.644.6%
Sample 224324325922024113.895.7%
Sample 387185497797291856.346.1%
*number of samples for each test n=16.

Gene/Protein Information For F11r (Source: Uniprot.Org, NCBI)

Uniprot ID

O88792

Gene ID

16456

Gene Name

F11r

Full Name

Junctional adhesion molecule A

Weight

32.424kDa

Superfamily

immunoglobulin superfamily

Alternative Names

CD321 antigen; CD321; F11 receptor; F11R; JAM-1; JAM1CD321; JAMA; JAM-A; JCAMJAM; Junctional adhesion molecule 1Platelet F11 receptor; junctional adhesion molecule A; PAM-1; PAM-1KAT; Platelet adhesion molecule 1 F11r|9130004G24, AA638916, ESTM3, ESTM33, JAM, JAM-, JAM-1, JAM-A, Jcam, Jcam1, Ly10, Ly106|F11 receptor|junctional adhesion molecule A|BV11 antigen|F11 receptor/F11R|junction cell adhesion molecule A|junction cell adhesion molecule1|junctional adhesion molecule 1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on F11r, check out the F11r Infographic

F11r infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for F11r: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1228

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11 Customer Q&As for Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™

Question

Q: can you suggest the dilution ratio of serum samples for detection of JAM-A in Mouse cell lysates? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

W. Chen

Verified customer

Asked: 2020-11-22

Answer

A: unable to know the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the JAM-A ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-11-22

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

M. Scott

Verified customer

Asked: 2019-08-07

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-08-07

Question

What are the normal levels of F11R seen in Naive animals (Murine) using EK1228?

Verified customer

Asked: 2019-04-18

Answer

For the Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ (EK1228), the normal levels of F11R seen in healthy mouse serum and plasma is 200-300pg/ml.

Boster Scientific Support

Answered: 2019-04-23

Question

What is the suggested dilution for serum and plasma samples with EK1228?

Verified customer

Asked: 2019-04-18

Answer

The suggested dilution for serum and plasma samples using the Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ (EK1228) is 1:1.

Boster Scientific Support

Answered: 2019-04-23

Question

Q: Can JAM-A ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

Verified Customer

Verified customer

Asked: 2019-01-12

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been evaluated by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-01-12

Question

Q: is it okay to use citrate plasma as samples in Mouse JAM-A Picokine® ELISA Kit (Catalog # EK1228)?

Verified Customer

Verified customer

Asked: 2018-08-30

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of JAM-A causing degradation of its protein structure. JAM-A may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the JAM-A ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2018-08-30

Question

Q: how do I analyze ELISA data? I have obtained JAM-A level in cell lysates.

Verified Customer

Verified customer

Asked: 2018-04-25

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-04-25

Question

I am planning on running the JAML-A/F11R ELISA in samples from animals in various models of murine IBD. What are the normal levels seen in Naive animals and what dilution do you suggest I start with?

Verified Customer

Verified customer

Asked: 2018-03-13

Answer

The normal levels of F11R seen in healthy mouse serum and plasma is

Boster Scientific Support

Answered: 2018-03-13

Question

Q: what is the protocol regarding preparation of cell lysates prepared for use in Picokine® ELISA kits?

B. Singh

Verified customer

Asked: 2016-07-30

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate can be found in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2016-07-30

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring JAM-A using the EK1228 Mouse JAM-A Picokine® ELISA Kit?

W. Zhou

Verified customer

Asked: 2016-02-17

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples in order to limit protein degradation.

Boster Scientific Support

Answered: 2016-02-17

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

V. Anand

Verified customer

Asked: 2015-12-19

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2015-12-19

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