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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse F11r in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1228)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: K27-G238
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1228 is reactive to F11r in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK1228 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1228-CAP||Anti-Mouse F11r Pre-coated 96-well strip microplate||1|
|EK1228-ST||Mouse F11r Standard||2 vials, 10 ng/tube|
|EK1228-DA||Mouse F11r Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse JAM-A/F11R PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect JAM-A, Dilution ratio of 1:1, concentration in serum is around 250pg/ml.
Some articles we found to cite concentrations of JAM-A in samples: 19258599 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of JAM-A in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For F11r (Source: Uniprot.Org, NCBI)
Junctional adhesion molecule A
CD321 antigen; CD321; F11 receptor; F11R; JAM-1; JAM1CD321; JAMA; JAM-A; JCAMJAM; Junctional adhesion molecule 1Platelet F11 receptor; junctional adhesion molecule A; PAM-1; PAM-1KAT; Platelet adhesion molecule 1 F11r|9130004G24, AA638916, ESTM3, ESTM33, JAM, JAM-, JAM-1, JAM-A, Jcam, Jcam1, Ly10, Ly106|F11 receptor|junctional adhesion molecule A|BV11 antigen|F11 receptor/F11R|junction cell adhesion molecule A|junction cell adhesion molecule1|junctional adhesion molecule 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on F11r, check out the F11r Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for F11r: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Mouse JAM-A / F11R / Junctional Adhesion Molecule 1 ELISA Kit PicoKine™ (EK1228)
No publications found for EK1228
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