Mouse Lumican ELISA Kit PicoKine™

Mouse Lumican ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Lum in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK1261
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Mouse Lumican ELISA Kit PicoKine™

See all Lumican products

SKU/Catalog Number

EK1261

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Lumican ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Lum in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse Lumican ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1261)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q19-N338

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1261 is reactive to Lum in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK1261 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1261-CAP Anti-Mouse Lum Pre-coated 96-well strip microplate 1
EK1261-ST Mouse Lum Standard 2 vials, 10 ng/tube
EK1261-DA Mouse Lum Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0840.1350.2710.3750.5880.9661.3661.920

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Lumican, Dilution ratio of 1:1000, concentration in serum is around 3221ng/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Lumican ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2401132556923511835831
Standard deviation14.8884.9328.5718.897431.49
CV (%)6.2%7.5%5.9%8%8.2%7.4%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Lumican in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 124025628526726216.386.2%
Sample 21132123211671292120561.375%
Sample 355694938556654895390263.214.8%
*number of samples for each test n=16.

Gene/Protein Information For Lum (Source: Uniprot.Org, NCBI)

Uniprot ID

P51885

Gene ID

17022

Gene Name

Lum

Full Name

Lumican

Weight

38.265kDa

Superfamily

small leucine-rich proteoglycan (SLRP) family

Alternative Names

KSPG lumican; LDC; LUM; lumican proteoglycan; Lumican; SLRR2D; SLRR2DLDCKeratan sulfate proteoglycan lumican Lum|Ld, Ldc, SL, SLRR2D|lumican|lumican|KSPG lumican|keratan sulfate proteoglycan lumican

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Lum, check out the Lum Infographic

Lum infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Lum: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

No publications found for EK1261

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8 Customer Q&As for Mouse Lumican ELISA Kit PicoKine™

Question

Would there be a problem with EK1261 to use plasma that was collected with EDTA as an anticoagulant?

Verified customer

Asked: 2020-09-17

Answer

For the Mouse Lumican ELISA Kit PicoKine™ (EK1261), EDTA plasma was not recommended due to the chelating properties. We suggest to collect plasma samples using heparin. If it is not possible, please run a comparison experiment by testing EDTA plasma and heparin plasma to see the effect. However, heparin is recommended.

Boster Scientific Support

Answered: 2020-09-17

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of Lumican in Mouse serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2020-01-09

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Lumican ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2020-01-09

Question

Q: is it okay to use citrate plasma as samples in Mouse Lumican Picokine® ELISA Kit (Catalog # EK1261)?

Verified Customer

Verified customer

Asked: 2019-09-19

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can attach metal ions from the functional domain of Lumican causing disruption of its protein structure. Lumican may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Lumican ELISA, treating samples with different anticoagulants and decided that heparin can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-09-19

Question

Q: how to proceed with the analysis of ELISA data? I have performed Lumican level in serum.

Verified Customer

Verified customer

Asked: 2019-09-09

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-09-09

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

S. Robinson

Verified customer

Asked: 2018-11-17

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-11-17

Question

Q: What is the optimal O.D. value for Lumican ELISA kit? I performed your Lumican ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Lumican even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2018-11-02

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. regarding your protocol, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Lumican ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Lumican that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2018-11-02

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification Lumican using the EK1261 Mouse Lumican Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-09-23

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2018-09-23

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2018-07-05

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-07-05

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