Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine™

Mouse MMP-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mmp3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10 pg/ml. Cited in 3 publication(s).

Product Info Summary

SKU: EK0462
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin).

Product Name

Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine™

See all MMP-3 products

SKU/Catalog Number

EK0462

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse MMP-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mmp3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0462)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Y18-C477

Sensitivity

<10 pg/ml

Assay Range

156 pg/ml - 10,000 pg/ml

Cross Reactivity

There is cross-reactivity with MMP-10 approximately 2% and no detectable cross-reactivity with other MMPs.

Reactive Species

EK0462 is reactive to Mmp3 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin).

Application Guarantee

EK0462 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0462-CAP Anti-Mouse Mmp3 Pre-coated 96-well strip microplate 1
EK0462-ST Mouse Mmp3 Standard 2 vials, 10 ng/tube
EK0462-DA Mouse Mmp3 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

Innovating Scientists Reward

If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

Submit A Review

Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015631262512502500500010000
O.D.0.0270.0970.1620.2650.4710.8031.3331.952

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect MMP-3, Dilution ratio of 1:40, concentration in serum is around 60ng/ml.

Some articles we found to cite concentrations of MMP-3 in samples: 24167138 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)14271427443230015244802
Standard deviation17.1468.49230.4621102.1288.12
CV (%)6.1%4.8%5.2%7%6.7%6%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of MMP-3 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 12812832712872805.892.1%
Sample 21427156916171589155073.314.7%
Sample 344324951478846844713188.463.9%
*number of samples for each test n=16.

Gene/Protein Information For Mmp3 (Source: Uniprot.Org, NCBI)

Uniprot ID

P28862

Gene ID

17392

Gene Name

Mmp3

Full Name

Stromelysin-1

Weight

53.845kDa

Superfamily

peptidase M10A family

Alternative Names

CHDS6; EC 3.4.24; EC 3.4.24.17; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MGC126102; MGC126103; MMP3; MMP-3; proteoglycanase; SL-1; STMY; STMY1MGC126104; STR1; Stromelysin 1; stromelysin-1; transin-1 Mmp3|EMS-2, MMP-3, S, SL, SL-1, SLN-1, SLN1, STR, STR-1, St, Stmy, Stmy1, Str1|matrix metallopeptidase 3|stromelysin-1|matrix metalloproteinase 3|progelatinase|transin-1

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Mmp3, check out the Mmp3 Infographic

Mmp3 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Mmp3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0462 has been cited in 3 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Xie J, Huo S, Li Y, Dai J, Xu H, Yin ZQ. Cell Transplant. 2017 Jun 9;26(6):967-982. doi: 10.3727/096368917X694994. Epub 2017 Feb 9. Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the M

Zhao J, Fan X, Zhang Q, Sun F, Li X, Xiong C, Zhang C, Fan H. J Biomed Mater Res A. 2014 Feb;102(2):373-80. Doi: 10.1002/Jbm.A.34711. Epub 2013 May 14. Chitosan-Plasmid Dna Nanoparticles Encoding Small Hairpin Rna Targeting Mmp-3 And -13 To Inhibi...

??gren Ms, Schnabel R, Christensen Lh, Mirastschijski U. Eur J Cell Biol. 2015 Jan;94(1):12-21. Doi: 10.1016/J.Ejcb.2014.10.001. Epub 2014 Oct 23. Tumor Necrosis Factor-??-Accelerated Degradation Of Type I Collagen In Human Skin Is Associated With...

Have you used Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine™?

Submit a review and receive an Amazon gift card.

  • $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
  • $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit A Review

Be the first to review Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine™

*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.

Have a question?

Find answers in Q&As, reviews.

Can't find your answer?

Submit your question

11 Customer Q&As for Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine™

Question

Q: if the enzyme conjugated MMP-3 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the MMP-3 antigen?

Verified Customer

Verified customer

Asked: 2020-05-26

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2020-05-26

Question

Is this kit reactive to pro-, active, and TIMP-complexed MMP3 protein?

Verified Customer

Verified customer

Asked: 2019-11-21

Answer

EK0462 is reactive to total MMP3 including pro-, active, and TIMP-complexed MMP3 protein.

Boster Scientific Support

Answered: 2019-11-21

Question

Q: which procedure should I follow in order to thaw whole blood sample for MMP-3 ELISA after freezing?

K. Babu

Verified customer

Asked: 2019-03-01

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test MMP-3 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2019-03-01

Question

Q: how do I analyze ELISA data? I have performed MMP-3 level in plasma.

J. Bhasin

Verified customer

Asked: 2018-12-30

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-12-30

Question

Q: can you tell me how to prepare cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-12-02

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-12-02

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

D. Phillips

Verified customer

Asked: 2018-07-10

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-07-10

Question

I am interested in your EK0462 MMP3 ELISA kit, may I know if this kit react to pro-, active, and TIMP-complexed MMP3 protein? And may I know what proteins you have tested with no cross reactivity concerned?

Verified Customer

Verified customer

Asked: 2018-07-04

Answer

EK0462 is reactive to total MMP3 including pro-, active, and TIMP-complexed MMP3 protein. The proteins listed below have been tested for cross-reactivity. Recombinant mouse MMP-2 protein Recombinant mouse MMP-9 protein Recombinant mouse TIMP-1 protein

Boster Scientific Support

Answered: 2018-07-04

Question

I am interested in your EK0462 MMP3 ELISA kit, may I know have you tested the kit with serum or plasma samples and may I know if the samples concentrations you tested by the kit and if you have suggested dilution ratio to use with this kit?

Verified Customer

Verified customer

Asked: 2018-05-29

Answer

The concentration range of MMP3 in serum and plasma samples is 1.5-30ng/ml. Recommended dilution ratio for normal serum and plasma samples is from 1:1 to 1:20.

Boster Scientific Support

Answered: 2018-05-29

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2017-07-05

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2017-07-05

Question

Q: Can MMP-3 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

T. Phillips

Verified customer

Asked: 2016-05-28

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2016-05-28

Question

What proteins have been tested for cross-reactivity?

Verified Customer

Verified customer

Asked: 2014-10-06

Answer

The proteins listed below have been tested for cross-reactivity. <br> Recombinant mouse MMP-2 protein <br> Recombinant mouse MMP-9 protein <br> Recombinant mouse TIMP-1 protein

Boster Scientific Support

Answered: 2014-10-06

$380

Ask a question

Ships directly to USA and Canada. For other regions, visit our distributors page

Bulk Inquiry

Sample Inquiry

In stock
Order Product
EK0462
$380.00

Troubleshooting

troubleshooting-box-image

Download troubleshooting handbooks for IHC, Western blot and ELISA for FREE.

Download Free PDFs Now

Boster Guarantee

Guaranteed product quality

Guaranteed product quality

We promise all of our products perform as described in datasheets.