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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin).|
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Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse MMP-3 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Mmp3 in cell culture supernatants, serum and plasma (heparin). Sensitivity: 10 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0462)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: Y18-C477
156 pg/ml - 10,000 pg/ml
There is cross-reactivity with MMP-10 approximately 2% and no detectable cross-reactivity with other MMPs.
EK0462 is reactive to Mmp3 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin).
EK0462 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of MMP-3
Matrix metalloproteinase-3 (MMP-3) also called stromelysin or transin, is a proteoglycanase closely related to collagenase (MMP1) with a wide range of substrate specificities. The complete primary structure for human MMP-3, which has 477 residues including a 17-residue signal peptide. MMP-3 and collagenase are 54% identical in sequence, suggesting a common origin for the evolution of the two proteinases. MMP-3 and collagenase expression are coordinately modulated in synovial fibroblast cultures. MMP-3 is a secreted metalloprotease produced predominantly by connective tissue cells. Together with other metalloproteases, it can synergistically degrade the major components of the extracellular matrix. It is capable of degrading proteoglycan, fibronectin, laminin, and type IV collagen, but not interstitial type I collagen. MMP-3 genotype may be an important determinant of vascular remodeling and age-related arterial stiffening, with the heterozygote having the optimal balance between matrix accumulation and deposition.
|EK0462-CAP||Anti-Mouse Mmp3 Pre-coated 96-well strip microplate||1|
|EK0462-ST||Mouse Mmp3 Standard||2 vials, 10 ng/tube|
|EK0462-DA||Mouse Mmp3 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse MMP-3 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect MMP-3, Dilution ratio of 1:40, concentration in serum is around 60ng/ml.
Some articles we found to cite concentrations of MMP-3 in samples: 24167138 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse MMP-3/Stromelysin-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of MMP-3 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Mmp3 (Source: Uniprot.Org, NCBI)
peptidase M10A family
CHDS6; EC 3.4.24; EC 184.108.40.206; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MGC126102; MGC126103; MMP3; MMP-3; proteoglycanase; SL-1; STMY; STMY1MGC126104; STR1; Stromelysin 1; stromelysin-1; transin-1 Mmp3|EMS-2, MMP-3, S, SL, SL-1, SLN-1, SLN1, STR, STR-1, St, Stmy, Stmy1, Str1|matrix metallopeptidase 3|stromelysin-1|matrix metalloproteinase 3|progelatinase|transin-1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Mmp3, check out the Mmp3 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Mmp3: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0462 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Xie J, Huo S, Li Y, Dai J, Xu H, Yin ZQ. Cell Transplant. 2017 Jun 9;26(6):967-982. doi: 10.3727/096368917X694994. Epub 2017 Feb 9. Olfactory Ensheathing Cells Inhibit Gliosis in Retinal Degeneration by Downregulation of the M
Zhao J, Fan X, Zhang Q, Sun F, Li X, Xiong C, Zhang C, Fan H. J Biomed Mater Res A. 2014 Feb;102(2):373-80. Doi: 10.1002/Jbm.A.34711. Epub 2013 May 14. Chitosan-Plasmid Dna Nanoparticles Encoding Small Hairpin Rna Targeting Mmp-3 And -13 To Inhibi...
??gren Ms, Schnabel R, Christensen Lh, Mirastschijski U. Eur J Cell Biol. 2015 Jan;94(1):12-21. Doi: 10.1016/J.Ejcb.2014.10.001. Epub 2014 Oct 23. Tumor Necrosis Factor-??-Accelerated Degradation Of Type I Collagen In Human Skin Is Associated With...
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