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Product Info Summary
| SKU: | EK0594 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, citrate). |
Product info
Product Name
Mouse Prolactin ELISA Kit PicoKine®
SKU/Catalog Number
EK0594
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Prolactin ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Prl in cell culture supernatants, serum and plasma (heparin, citrate). Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Prolactin ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0594)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: L32-C228
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from rat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
156 pg/ml - 10,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0594 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0594 is reactive to Prl in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, citrate).
Application Guarantee
EK0594 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Prl
Prolactin, also calle PRL, is a protein that in humans is encodes by the PRL gene. This gene is mapped to 6p22.3. This gene encodes the anterior pituitary hormone prolactin. It may play a role in cell survival by suppressing apoptosis, and it is essential for lactation. Prolactin also acts in a cytokine-like manner and as an important regulator of the immune system. Prolactin has important cell cycle related functions as a growth-, differentiating- and anti-apoptotic factor. As a growth factor binding to cytokine like receptors it has also profound influence on hematopoiesis, angiogenesis and is involved in the regulation of blood clotting through several pathways.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0594-CAP | Anti-Mouse Prl Pre-coated 96-well strip microplate | 1 |
| EK0594-ST | Mouse Prl Standard | 2 vials, 10 ng/tube |
| EK0594-DA | Mouse Prl Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 156 | 312 | 625 | 1250 | 2500 | 5000 | 10000 |
| O.D. | 0.003 | 0.067 | 0.14 | 0.282 | 0.585 | 1.12 | 1.76 | 2.209 |
Data Example Images
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Mouse Prolactin PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Prl, Dilution ratio of 1:5, concentration in serum and plasma is 6-50 ng/ml..
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Prolactin ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 247 | 1548 | 4124 | 254 | 1402 | 4314 |
| Standard deviation | 13.83 | 68.11 | 206.2 | 15.74 | 82.71 | 228.64 |
| CV (%) | 4.4%% | 4.4% | 5% | 6.2% | 5.9% | 5.3% |
Specific Publications For Mouse Prolactin ELISA Kit PicoKine® (EK0594)
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Recommended Resources
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Customer Reviews
0 Reviews For Mouse Prolactin ELISA Kit PicoKine®
Customer Q&As
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13 Customer Q&As for Mouse Prolactin ELISA Kit PicoKine®
Question
What samples were used to test the specificity of EK0594? BalbC mouse serum?
Verified customer
Asked: 2021-02-26
Answer
Our lab used BalbC mouse serum to test the specificity of the Mouse Prolactin ELISA Kit PicoKine™ (EK0594).
Boster Scientific Support
Answered: 2021-03-01
Question
Does EK0594 react with other serum (other mouse strains, rat or human)?
Verified customer
Asked: 2021-02-26
Answer
For the Mouse Prolactin ELISA Kit PicoKine™ (EK0594), our lab didn't test other serum from other mouse strains, rat or human.
Boster Scientific Support
Answered: 2021-03-01
Question
Which recombinant proteins were used to test the cross-reactivity of EK0594?
Verified customer
Asked: 2021-02-26
Answer
The following factors were assayed using the Mouse Prolactin ELISA Kit PicoKine™ (EK0594) and exhibited no cross-reactivity: Recombinant mouse Prolactin R protein Recombinant rat Prolactin R protein Recombinant human Prolactin R protein It has 0.9% cross-reactivity with recombinant human Prolactin protein.
Boster Scientific Support
Answered: 2021-03-01
Question
What is the EK0594 antibody targeting within the recombinant mouse prolactin sequence (i.e. which amino acids)?
Verified customer
Asked: 2021-01-26
Answer
The immunogen sequence of antibody pairs in the Mouse Prolactin ELISA Kit PicoKine™ (EK0594) is L32-C228(Uniprot ID:P06879).
Boster Scientific Support
Answered: 2021-03-01
Question
Q: how do I analyze ELISA data? I have obtained Prolactin level in serum.
Verified Customer
Verified customer
Asked: 2020-03-23
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/elisa-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/elisa-data-analysis-online
Boster Scientific Support
Answered: 2020-03-23
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2020-02-29
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2020-02-29
Question
Has EK0594 been tested for cross-reactivity with rat prolactin?
Verified customer
Asked: 2019-10-03
Answer
For the Mouse Prolactin ELISA Kit PicoKine™ (EK0594), our lab has not tested cross reactivity with rat. Please see the sequence homology with rat, https://www.uniprot.org/blast/uniprot/B20191016216DA2B77BFBD2E6699CA9B6D1C41EB20C2AD9C
Boster Scientific Support
Answered: 2019-10-08
Question
Q: how to thaw whole blood sample for Prolactin ELISA after freezing?
M. Roberts
Verified customer
Asked: 2019-08-29
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test Prolactin for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2019-08-29
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
M. Yang
Verified customer
Asked: 2018-12-08
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-12-08
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2018-09-03
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2018-09-03
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measurement Prolactin using the EK0594 Mouse Prolactin Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2017-11-16
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.
Boster Scientific Support
Answered: 2017-11-16
Question
Q: What is the optimal O.D. value for Prolactin ELISA kit? I used your Prolactin ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Prolactin even though the O.D. values are not very high?
Y. Liu
Verified customer
Asked: 2015-01-24
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Prolactin ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Prolactin that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2015-01-24
Question
Q: Can Prolactin ELISA Kits be used with tissue homogenates (or other non-validated sample types)?
R. Bhatt
Verified customer
Asked: 2014-05-13
Answer
A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not valid for other sample types than we have tested: it means further investigation is a must. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.
Boster Scientific Support
Answered: 2014-05-13
