Mouse Rat Estradiol ELISA Kit
Competitive High Sensitivity ELISA kit for Quantitative Detection of Mouse/Rat Estradiol. 96wells/kit, with removable strips. Cited in 1 publication(s).
Mouse Rat Estradiol ELISA Kit Info At A Glance
|Sample Type:||Serum and plasma|
|Sample Volume:||100μl per well|
|Assay Range:||3-300 pg/ml|
This ELISA kit is based on the principle of competitive binding between E2 in the test specimen and E2 enzyme conjugate for a constant amount of anti-Estradiol polyclonal antibody. In the incubation, anti-E2 antibody coated wells are incubated with E2 standards, controls, samples, and E2 enzyme conjugate at room temperature for 60 minutes. During the incubation, a fixed amount of HRPlabeled E2 competes with the endogenous E2 in the standard, sample, or quality control serum for a fixed number of binding sites of the specific E2 antibody. E2 peroxidase conjugate immunologically bound to the well progressively decreases as the concentration of E2 in the specimen increases. Unbound E2 peroxidase conjugate is then removed and the wells are washed. Next, a solution of TMB Reagent is added and incubated at room temperature for 30 minutes, resulting in the development of blue color. The color development is stopped with the addition of stop solution, and the absorbance is measured spectrophotometrically at 450 nm. A standard curve is obtained by plotting the concentration of the standard versus the absorbance
|Product Name||Mouse Rat Estradiol ELISA Kit
See all HSD17B1 primary antibodies, ELISA kits and proteins
|Storage & Handling||Store the kit at 2°C to 8°C. Keep microwells sealed in a dry bag with desiccants. The reagents are stable until expiration of the kit. Do not expose reagent to heat, sun, or strong light. Avoid multiple freeze-thaw cycles(Shipped with wet ice.)|
|Description||Competitive High Sensitivity ELISA kit for Quantitative Detection of Mouse/Rat Estradiol. 96wells/kit, with removable strips.|
|Cite This Product||Mouse Rat Estradiol ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK7003)|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||Serum and plasma|
|Assay Range||3-300 pg/ml|
|Cross Reactivity||There is no detectable cross-reactivity.|
Protein Target Info (Source: Uniprot.org)
*if product is indicated to react with multiple species, protein info is based on the human gene.
|Protein Function||Estradiol E2 is the most potent natural Estrogen, produced mainly by the ovary, placenta, and in smaller amounts by the adrenal cortex, and the male testes. Estradiol is secreted into the blood stream where 98% bound to sex hormone binding globulin (SHBG). Estrogenic activity is effected via estradiol-receptor complexes which trigger the appropriate response at the follicles, uterus, breast, vagina, urethra, hypothalamus, pituitary and to a lesser extent the liver and skin. In non-pregnant women with normal menstrual cycles, estradiol secretion follows a cyclic, biphasic pattern with the highest concentration found immediately prior to ovulation. During pregnancy, maternal serum Estradiol levels increase considerably, to well above the pre-ovulatory peak levels and high levels are sustained throughout pregnancy. Serum Estradiol measurements are a valuable index in evaluating a variety of menstrual dysfunctions such as precocious or delayed puberty in girls and primary and secondary amenorrhea and menopause. Estradiol levels have been reported to be increased in patients with feminizing syndromes, gynaecomastia and testicular tumors. In cases of infertility, serum Estradiol measurements are useful for monitoring induction of ovulation following treatment.|
|Background||Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone.|
Kit Components And Assay QC Data Details Of Mouse Rat Estradiol ELISA Kit
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|Microwells coated with polyclonal anti-Estradiol Antibody||12x8x1 Microwells|
|Estradiol Standards||6 vials ( ready to use) 0.5 ml|
|Estradiol Enzyme conjugate Concentrate, 20X||1 vial 0.7ml|
|Assay Diluent||1 bottle (ready to use) 12ml|
|TMB Reagent||1 bottle (ready to use) 12ml|
|Stop Solution||1 bottle (ready to use) 12ml|
|20X Wash concentrate||1 bottle 25ml|
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK7003 Mouse Rat Estradiol ELISA Kit. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 30 minutesConcentration(pg/ml)|0|10|30|100|300|1000 O.D.|2.069|1.623|1.292|0.794|0.388|0.162
We measured random samples of Mouse Rat Estradiol ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
We measured the performance consistency of Mouse Rat Estradiol ELISA Kit to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 1
|The Extracts of Pacific Oyster (Crassostrea Gigas) Alleviate Ovarian Functional Disorders of Female Rats with Exposure to Bisphenol a Through Decreasing FSHR Expression in Ovarian Tissues||PubMed ID 25395697|
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