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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum, plasma (heparin, EDTA) and urine.|
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Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Rbp4 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0832)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: E19-L201
312 pg/ml - 20,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0832 is reactive to Rbp4 in Mouse samples
Validated Sample Types
cell culture supernatants, serum, plasma (heparin, EDTA) and urine.
EK0832 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of RBP4/Retinol-Binding Protein 4
Retinol binding protein 4, plasma, also known as RBP4, belongs to the lipocalin family and is the specific carrier for retinol (vitamin A alcohol) in the blood. It is protein that it is encoded by the RBP4 gene. RBP4 gene resides just centromeric of the cluster of CYP2C genes on 10q24. The mouse Rbp4 locus is closely linked and just proximal to the locus for phenobarbital-inducible cytochrome P450-2c (Cyp-2c) at the distal end of chromosome 19. It delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP-retinol complex interacts with transthyretin, which prevents its loss by filtration through the kidney glomeruli. A deficiency of vitamin A blocks secretion of the binding protein posttranslationally and results in defective delivery and supply to the epidermal cells. The standard product used in this kit are recombinant mouse B7-1/CD80, D37—K245, consisting of dimer acids with two single straned. The standard used in this kit is recombinant protein, with E19-L201 aa sequence, the molecular weight is 22kda.
|EK0832-CAP||Anti-Mouse Rbp4 Pre-coated 96-well strip microplate||1|
|EK0832-ST||Mouse Rbp4 Standard||2 vials, 20 ng/tube|
|EK0832-DA||Mouse Rbp4 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse RBP4 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect RBP4/Retinol-Binding Protein 4, Dilution ratio of 1:500, concentration in serum is 1-2ug/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of RBP4/Retinol-Binding Protein 4 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Rbp4 (Source: Uniprot.Org, NCBI)
Retinol-binding protein 4
interstitial; Plasma retinol-binding protein; RBP4; retinol binding protein 4, plasma; RetinolBinding Protein 4; retinol-binding protein 4, plasma RBP4 MCOPCB10, RDCCAS retinol binding protein 4 retinol-binding protein 4|PRBP|RBP|plasma retinol-binding protein|retinol binding protein 4, plasma|retinol-binding protein 4, interstitial*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Rbp4, check out the Rbp4 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Rbp4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0832 has been cited in 2 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
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