Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™

Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Rbp4 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK0832
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Product Name

Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™

See all RBP4/Retinol-Binding Protein 4 products

SKU/Catalog Number

EK0832

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Rbp4 in cell culture supernatants, serum, plasma (heparin, EDTA) and urine. Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0832)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: E19-L201

Sensitivity

<10 pg/ml

Assay Range

312 pg/ml - 20,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0832 is reactive to Rbp4 in Mouse samples

Validated Sample Types

cell culture supernatants, serum, plasma (heparin, EDTA) and urine.

Application Guarantee

EK0832 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0832-CAP Anti-Mouse Rbp4 Pre-coated 96-well strip microplate 1
EK0832-ST Mouse Rbp4 Standard 2 vials, 20 ng/tube
EK0832-DA Mouse Rbp4 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)03126251250250050001000020000
O.D.0.0900.1410.1920.3000.5210.9391.6772.337

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect RBP4/Retinol-Binding Protein 4, Dilution ratio of 1:500, concentration in serum is 1-2ug/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)4182343843540125767635
Standard deviation23.4105.43649.4928.07159.71633.7
CV (%)4.5%4.5%7.7%7%6.2%8.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of RBP4/Retinol-Binding Protein 4 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 141842143038941415.373.7%
Sample 223432347251226672467133.975.4%
Sample 384358668989794949123595.26.5%
*number of samples for each test n=16.

Gene/Protein Information For Rbp4 (Source: Uniprot.Org, NCBI)

Uniprot ID

P02753

Gene ID

5950

Gene Name

Rbp4

Full Name

Retinol-binding protein 4

Weight

23.01kDa

Superfamily

calycin superfamily

Alternative Names

interstitial; Plasma retinol-binding protein; RBP4; retinol binding protein 4, plasma; RetinolBinding Protein 4; retinol-binding protein 4, plasma RBP4 MCOPCB10, RDCCAS retinol binding protein 4 retinol-binding protein 4|PRBP|RBP|plasma retinol-binding protein|retinol binding protein 4, plasma|retinol-binding protein 4, interstitial

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Rbp4, check out the Rbp4 Infographic

Rbp4 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Rbp4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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9 Customer Q&As for Mouse RBP4/Retinol Binding Protein 4 ELISA Kit PicoKine™

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for analysis RBP4 using the EK0832 Mouse RBP4 Picokine® ELISA Kit?

C. Turner

Verified customer

Asked: 2020-05-15

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may alter the stabilityof samples. It is recommend to assay sample on priority basis collection when possible, or aliquot into single use volumes and store samples frozen. avoid repetitive freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2020-05-15

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2019-09-25

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2019-09-25

Question

Q: how to analyze ELISA data? I have obtained RBP4 level in plasma.

Verified Customer

Verified customer

Asked: 2019-08-11

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2019-08-11

Question

Q: Can RBP4 ELISA Kits be used with tissue homogenates (or other non-validated sample types)?

C. Bhatt

Verified customer

Asked: 2019-05-26

Answer

A: Unfortunately, Boster Bio has not routinely validated tissue homogenates as a sample type for ELISA kits. This does not mean that ELISA kits are not suitable for other sample types than we have tested: it means further investigation is required. One will need to perform a spike and recovery study to determine if an unvalidated sample type will work with a particular kit. To perform a spike and recovery experiment, one should divide a sample into two aliquots. In one of the aliquots, the user should spike in a known amount of the kit standard. a dilution series is performed comparing the spiked versus the unspiked sample. Generally, samples with expected recovery and linearity between 80-120% are considered acceptable. This method can be used to validate any sample type that has not been assessd by Boster Bio. for a more detailed spike and recovery protocol, please contact technical support.
Note: acceptable ranges should be determined individually by each laboratory. Additionally, technical support can help determine if a buffer component is not compatible with a given ELISA kit. please see the Citations tab on the product webpage for peer-reviewed papers utilizing a wide range of sample types. We also have an innovator's reward program where if the user validates our ELISA kits in applications or samples previously not validated by Boster Bio or other users, and share such information with us by submit a review, we will reward the user's efforts with a free antibody or ELISA kit from our catalog. Biocompare.com will also give $20 Amazon giftcard as an additional reward, if the review is submitted there as well.

Boster Scientific Support

Answered: 2019-05-26

Question

Q: if the enzyme conjugated RBP4 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the RBP4 antigen?

Verified Customer

Verified customer

Asked: 2019-04-14

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2019-04-14

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2019-02-17

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is a must for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2019-02-17

Question

Q: which procedure should I follow in order to thaw whole blood sample for RBP4 ELISA after freezing?

G. Adams

Verified customer

Asked: 2017-05-03

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test RBP4 for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2017-05-03

Question

Q: how much samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2017-03-28

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2017-03-28

Question

Q: can I use citrate plasma as samples in Mouse RBP4 Picokine® ELISA Kit (Catalog # EK0832)?

Verified Customer

Verified customer

Asked: 2015-08-17

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of RBP4 causing disruption of its protein structure. RBP4 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the RBP4 ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2015-08-17

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