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Mouse Renin 1 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Mouse Renin-1. 96wells/kit, with removable strips. Cited in 1 publication(s).
Mouse Renin 1 ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK1250 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Mouse |
| Sample Type: | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <10pg/ml |
| Assay Range: | 62.5pg/ml-4000pg/ml |
Overview
| Product Name | Mouse Renin 1 ELISA Kit PicoKine™
See all Renin primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK1250 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Mouse Renin/REN with <10pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Renin/REN tissue specificity: Kidney. |
| Cite This Product | Mouse Renin 1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1250) |
Product Specs
| Reactivity/Species | Mouse |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity | <10pg/ml |
| Assay Range | 62.5pg/ml-4000pg/ml |
| Immunogen/Standard | Expression system for standard: NSO; Immunogen sequence: S72-R402 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from rat|polyclonal antibody from goat |
| Cross Reactivity | There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size | 96wells/kit, with removable strips. |
Gene/Protein Basic Information For Ren1 (Source: Uniprot.org, NCBI)
| Uniprot Id | P06281 |
|---|---|
| NCBI Gene Id | 19701 |
| Species Of This Entry | Mouse |
| Gene Name | Ren1 |
| Protein Name | Renin-1 |
| Superfamily | peptidase A1 family |
| Alternative Names | Renin|angiotensin-forming enzyme; Angiotensinogenase; EC 3.4.23; EC 3.4.23.15; FLJ10761; HNFJ2; REN; renin precursor, renal; Renin |
| Molecular Weight | 44343 |
| Post Tranlational Modifications | Cleavage; Phosphorylation; Ubiquitination; Glycosylation; Deacetylation; Acetylation; Oxidation; Methylation; Dephosphorylation; Biotinylation; Reduction; Deglycosylation; Myristoylation |
| Gene Location | 1 E4|1 57.91 cM, on Chromasome 1, gene sequence: |
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Ren1 (Source: Uniprot.org, NCBI)
| Protein Function | Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. |
|---|---|
| Tissue Specificity | Kidney. |
| Related Diseases | Hypertensive Disease; Neoplasms; Malignant Neoplasms; Mesothelioma; Malignant Paraganglionic Neoplasm; Kidney Diseases; Essential Hypertension; Malignant Mesothelioma; Cell Transformation, Neoplastic; Pleural Neoplasms; Hypertrophy; Medulloblastoma; Hemorrhage; Carcinoma; Nervousness; Pleural Mesothelioma; Malignant Pleural Mesothelioma; Dysequilibrium Syndrome; Cardiovascular Diseases; Malignant Neoplasm Of Breast |
| Related Pathways | Pathogenesis; Localization; Secretion; Transport; Cell Proliferation; Excretion; Cell Death; Oncogenesis; Glycosylation; Ovulation; Aging; Translation; Proprioception; Vasodilation; Detection Of Glucose; Ossification; Tropism; Reflex; Cell Differentiation; Chromatin Remodeling |
| Background | Renin-1 is also known as Ren, Angiotensinogenase or Kidney rennin. It is a member of peptidase A1 family. Renin is a highly specific endopeptidase, whose only known function is to generate angiotensin I from angiotensinogen in the plasma, initiating a cascade of reactions that produce an elevation of blood pressure and increased sodium retention by the kidney. It associated to membranes via binding to ATP6AP2. |
| Scientific References | PMID: 6370686 by Holm I., et al. Evolution of aspartyl proteases by gene duplication: the mouse renin gene is organized in two homologous clusters of four exons. PMID: 2685761 by Kim W.S., et al. Nucleotide sequence of a cDNA coding for mouse Ren1 preprorenin. |
Kit Components And Assay QC Data Details Of Mouse Renin 1 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK1250-CAP | 96-well plate precoated with anti-Mouse Renin antibody | 1 |
| EK1250-ST | lyophilized recombinant Mouse Renin standard | 10ng/tube |
| EK1250-DA | biotinylated anti-Mouse Renin antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK1250 Mouse Renin 1 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.056 | 0.195 | 0.235 | 0.379 | 0.578 | 1.061 | 1.734 | 2.377 |
We measured random samples of Mouse Renin 1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 158 | 449 | 1246 | 147 | 482 | 1262 |
| Standard deviation | 10.74 | 28.73 | 64.79 | 11.9 | 34.22 | 70.67 |
| CV(%) | 6.8% | 6.4% | 5.2% | 8.1% | 7.1% | 5.6% |
We measured the performance consistency of Mouse Renin 1 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 158 | 161 | 142 | 150 | 152 | 7.39 | 4.8% |
| Sample 2 | 449 | 465 | 437 | 476 | 456 | 14.9 | 3.2% |
| Sample 3 | 1246 | 1215 | 1179 | 1158 | 1199 | 33.7 | 2.8% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all Renin primary antibodies,Other Recommended Resources
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Total number of citations: 1
| Wang C, Ren X. Hybridoma (Larchmt). 2010 Dec;29(6):511-6. Doi: 10.1089/Hyb.2010.0044. Epub 2010 Nov 18. Preparation And Characterization Of Polyclonal Antibody Against Severe Acute Respiratory Syndrome-Associated Coronavirus Spike Protein. | PubMed ID 21087096 |
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