Mouse Sclerostin/SOST ELISA Kit PicoKine™

Mouse Sclerostin/SOST ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Sost in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml. Cited in 1 publication(s).

Product Info Summary

SKU: EK1179
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Mouse Sclerostin/SOST ELISA Kit PicoKine™

See all SOST/Sclerostin products

SKU/Catalog Number

EK1179

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse Sclerostin/SOST ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Sost in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Mouse Sclerostin/SOST ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1179)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: Q24-Y211

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK1179 is reactive to Sost in Mouse samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK1179 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK1179-CAP Anti-Mouse Sost Pre-coated 96-well strip microplate 1
EK1179-ST Mouse Sost Standard 2 vials, 10 ng/tube
EK1179-DA Mouse Sost Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0550.1010.1380.2010.4260.7121.4662.085

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect SOST/Sclerostin, Dilution ratio of 1:1, concentration in serum is around 200pg/ml.

Some articles we found to cite concentrations of SOST/Sclerostin in samples: 23044658 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse Sclerostin/SOST ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)3019043828192398
Standard deviation2.0413.4922.772.2415.5528.05
CV (%)6.8%7.1%5.2%8%8.1%7.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of SOST/Sclerostin in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 130313230300.822.7%
Sample 21901791791811824.542.4%
Sample 343850346949547625.415.3%
*number of samples for each test n=16.

Gene/Protein Information For Sost (Source: Uniprot.Org, NCBI)

Uniprot ID

Q99P68

Gene ID

74499

Gene Name

Sost

Full Name

Sclerostin

Weight

23.501kDa

Superfamily

sclerostin family

Alternative Names

sclerostin; SOST; VBCHsclerosteosis Sost|5430411E23Rik|sclerostin|sclerostin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Sost, check out the Sost Infographic

Sost infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Sost: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK1179 has been cited in 1 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Sclerostin Promotes Bone Remodeling in the Process of Tooth Movement

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9 Customer Q&As for Mouse Sclerostin/SOST ELISA Kit PicoKine™

Question

Q: is it okay to use citrate plasma as samples in Mouse SOST Picokine® ELISA Kit (Catalog # EK1179)?

Verified Customer

Verified customer

Asked: 2020-10-31

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of SOST causing disruption of its protein structure. SOST may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the SOST ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2020-10-31

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-12-25

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-12-25

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification SOST using the EK1179 Mouse SOST Picokine® ELISA Kit?

Z. Adams

Verified customer

Asked: 2018-05-25

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2018-05-25

Question

Q: if the enzyme conjugated SOST antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the SOST antigen?

T. Clark

Verified customer

Asked: 2018-03-24

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2018-03-24

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

D. Hall

Verified customer

Asked: 2017-02-18

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2017-02-18

Question

Q: What is the optimal O.D. value for SOST ELISA kit? I used your SOST ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain SOST even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2016-11-26

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this SOST ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of SOST that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2016-11-26

Question

Q: how to analyze ELISA data? I measured SOST level in plasma.

Z. Baker

Verified customer

Asked: 2016-07-09

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2016-07-09

Question

Q: how to thaw whole blood sample for SOST ELISA after freezing?

G. Banerjee

Verified customer

Asked: 2016-05-02

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test SOST for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2016-05-02

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2014-07-18

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2014-07-18

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