Product Info Summary
SKU: | EK1179 |
---|---|
Size: | 96 wells/kit, with removable strips. |
Reactive Species: | Mouse |
Application: | ELISA |
Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
Product info
Product Name
Mouse Sclerostin/SOST ELISA Kit PicoKine®
View all SOST/Sclerostin ELISA kits
SKU/Catalog Number
EK1179
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse Sclerostin/SOST ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Sost in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse Sclerostin/SOST ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1179)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: NS0; Immunogen sequence: Q24-Y211
Sensitivity
<10 pg/ml
Assay Range
15.6 pg/ml - 1,000 pg/ml
Standard Dilution Instructions
Add 100ul of sample diluent in well #2-#8. Add 200ul standard stock solution to well #1, and serial dilute for well #2-#7 to make a standard curve row. Leave well #8 as blank See datasheet of EK1179 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK1179 is reactive to SOST in Mouse samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK1179 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of SOST/Sclerostin
Sclerostin is a protein that in humans is encoded by the SOST gene. Sclerostin is a secreted glycoprotein with a C-terminal cysteine knot-like (CTCK) domain and sequence similarity to the DAN (differential screening-selected gene aberrative in neuroblastoma) family of bone morphogenetic protein (BMP) antagonists. Sclerostin is produced by the osteocyte and has anti-anabolic effects on bone formation. The SOST gene maps to chromosome 17q12-q21
Kit Components
Catalog Number | Description | Quantity |
---|---|---|
EK1179-CAP | Anti-Mouse SOST Pre-coated 96-well strip microplate | 1 |
EK1179-ST | Mouse SOST Standard | 2 vials, 10 ng/tube |
EK1179-DA | Mouse SOST Biotinylated antibody (100x) | 100ul |
AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
AR1106-1 | Sample Diluent | 30ml |
AR1106-2 | Antibody Diluent | 12ml |
AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
AR1104 | Color Developing Reagent (TMB) | 10ml |
AR1105 | Stop Solution | 10ml |
AR1106-5 | Wash Buffer (25x) | 20ml |
PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
Concentration (pg/ml) | 0 | 15.6 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 |
O.D. | 0.055 | 0.101 | 0.138 | 0.201 | 0.426 | 0.712 | 1.466 | 2.085 |
Data Example Images
Click image to see more details
Mouse Sclerostin/SOST PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect SOST/Sclerostin, Dilution ratio of 1:1, concentration in serum and plasma is around 200 pg/ml..
Some articles we found to cite concentrations of SOST/Sclerostin in samples: 23044658 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse Sclerostin/SOST ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
Intra-Assay Precision | Inter-Assay Precision | |||||
---|---|---|---|---|---|---|
Sample | 1 | 2 | 3 | 1 | 2 | 3 |
n | 16 | 16 | 16 | 24 | 24 | 24 |
Mean (pg/ml) | 30 | 190 | 438 | 28 | 192 | 398 |
Standard deviation | 2.04 | 13.49 | 22.77 | 2.24 | 15.55 | 28.05 |
CV (%) | 6.8% | 7.1% | 5.2% | 8% | 8.1% | 7.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of SOST/Sclerostin in ELISA kits from four different production batches/lots.
Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
---|---|---|---|---|---|---|---|
Sample 1 | 30 | 31 | 32 | 30 | 30 | 0.82 | 2.7% |
Sample 2 | 190 | 179 | 179 | 181 | 182 | 4.54 | 2.4% |
Sample 3 | 438 | 503 | 469 | 495 | 476 | 25.41 | 5.3% |
Protein Target Info & Infographic
Gene/Protein Information For SOST (Source: Uniprot.Org, NCBI)
Gene Name
SOST
Full Name
Sclerostin
Weight
24.031kDa
Superfamily
sclerostin family
Alternative Names
sclerostin; SOST; VBCHsclerosteosis SOST CDD, DAND61, VBCH, SOST sclerostin sclerostin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on SOST, check out the SOST Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for SOST: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected].
Specific Publications For Mouse Sclerostin/SOST ELISA Kit PicoKine® (EK1179)
Hello CJ!
EK1179 has been cited in 3 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Sclerostin Promotes Bone Remodeling in the Process of Tooth Movement
Regulation of osteoblast differentiation by osteocytes cultured on sclerostin antibody conjugated TiO2 nanotube array
Sclerostin Promotes Bone Remodeling in the Process of Tooth Movement
Recommended Resources
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Customer Reviews
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Customer Q&As
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Find answers in Q&As, reviews.
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9 Customer Q&As for Mouse Sclerostin/SOST ELISA Kit PicoKine®
Question
Q: is it okay to use citrate plasma as samples in Mouse SOST Picokine® ELISA Kit (Catalog # EK1179)?
Verified Customer
Verified customer
Asked: 2020-10-31
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of SOST causing disruption of its protein structure. SOST may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the SOST ELISA, treating samples with different anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2020-10-31
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2019-12-25
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-12-25
Question
Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for quantification SOST using the EK1179 Mouse SOST Picokine® ELISA Kit?
Z. Adams
Verified customer
Asked: 2018-05-25
Answer
A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. limit repetitive freeze-thaw cycles with the stored samples to prevent protein degradation.
Boster Scientific Support
Answered: 2018-05-25
Question
Q: if the enzyme conjugated SOST antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the SOST antigen?
T. Clark
Verified customer
Asked: 2018-03-24
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2018-03-24
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
D. Hall
Verified customer
Asked: 2017-02-18
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2017-02-18
Question
Q: What is the optimal O.D. value for SOST ELISA kit? I used your SOST ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain SOST even though the O.D. values are not very high?
Verified Customer
Verified customer
Asked: 2016-11-26
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this SOST ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of SOST that can be declared/interpreted as positive by the above standard is 10pg/ml.
Boster Scientific Support
Answered: 2016-11-26
Question
Q: how to analyze ELISA data? I measured SOST level in plasma.
Z. Baker
Verified customer
Asked: 2016-07-09
Answer
A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2016-07-09
Question
Q: how to thaw whole blood sample for SOST ELISA after freezing?
G. Banerjee
Verified customer
Asked: 2016-05-02
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test SOST for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2016-05-02
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2014-07-18
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer may affect immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2014-07-18