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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, serum and plasma (heparin, EDTA).|
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Mouse TIE2/TEK ELISA Kit PicoKine®
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Mouse TIE2 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tek in cell culture supernatants, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse TIE2/TEK ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0976)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: NS0; Immunogen sequence: A23-K744
125 pg/ml - 8,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0976 is reactive to Tek in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA).
EK0976 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Tie-2
Angiopoietin-1 receptor, also called TEK or TIE2 is a protein that in humans is encoded by the TEK gene. This gene is mapped to 9p21.2. The TEK receptor tyrosine kinase is expressed almost exclusively in endothelial cells in mice, rats, and humans. This receptor possesses a unique extracellular domain containing 2 immunoglobulin-like loops separated by 3 epidermal growth factor-like repeats that are connected to 3 fibronectin type III-like repeats. The ligand for the receptor is angiopoietin-1. Defects in TEK are associated with inherited venous malformations, the TEK signaling pathway appears to be critical for endothelial cell-smooth muscle cell communication in venous morphogenesis. TEK is closely related to the TIE receptor tyrosine kinase.
|EK0976-CAP||Anti-Mouse Tek Pre-coated 96-well strip microplate||1|
|EK0976-ST||Mouse Tek Standard||2 vials, 10 ng/tube|
|EK0976-DA||Mouse Tek Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Mouse TEK/TIE2 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Tie-2, Dilution ratio of 1:10 , concentration in serum is around 10ng/ml .
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse TIE2/TEK ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Tie-2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Tek (Source: Uniprot.Org, NCBI)
protein kinase superfamily
angiopoietin-1 receptor; CD202b antigen; CD202b; EC 2.7.10; EC 188.8.131.52; hTIE2; p140 TEK; soluble TIE2 variant 1; soluble TIE2 variant 2; TEK tyrosine kinase, endothelial; TEK; Tie2; Tie-2; TIE2CD202b; Tunica interna endothelial cell kinase; Tyrosine-protein kinase receptor TEK; Tyrosine-protein kinase receptor TIE-2; venous malformations, multiple cutaneous and mucosal; VMCM; VMCM1 Tek|AA517024, Cd202b, Hyk, STK1, Tie, Tie-2, Tie2, tie-|TEK receptor tyrosine kinase|angiopoietin-1 receptor|endothelial tyrosine kinase|endothelial-specific receptor tyrosine kinase|p140 TEK|tunica interna endothelial cell kinase|tyrosine kinase with Ig and EGF homology domains-2|tyrosine-protein kinase receptor TEK|tyrosine-protein kinase receptor TIE-2*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Tek, check out the Tek Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Tek: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0976 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Sancakdar E, Guven As, Uysal Eb, Deveci K, G??lt??rk E. Pediatr Infect Dis J. 2015 Aug;34(8):E200-5. Doi: 10.1097/Inf.0000000000000706. Important Of Angiopoietic System In Evaluation Of Endothelial Damage In Children With Crimean-Congo Hemorrhagic...
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