Mouse TNFRH3/TNFRSF26 ELISA Kit PicoKine®

Tnfrsf26 ELISA kit for Mouse

Mouse TNFRH3/TNFRSF26 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf26 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Product Info Summary

SKU: EK2009
Size: 96 wells/kit, with removable strips.
Reactive Species: Mouse
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

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Product Name

Mouse TNFRH3/TNFRSF26 ELISA Kit PicoKine®

View all Tnfrsf26 ELISA kits

SKU/Catalog Number

EK2009

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Mouse TNFRH3/TNFRSF26 ELISA Kit PicoKine™ (96 Tests). Quantitate Mouse Tnfrsf26 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 10pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Mouse TNFRH3/TNFRSF26 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK2009)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: NS0; Immunogen sequence: S20-L164

Sensitivity

<10 pg/ml

Assay Range

15.6 pg/ml - 1,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK2009 is reactive to Tnfrsf26 in Mouse samples

Validated Sample Types

cell culture supernatants, serum and plasma (heparin, EDTA, citrate).

Application Guarantee

EK2009 is guaranteed for ELISA in Mouse by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of Tnfrsf26

Tumor necrosis factor receptor homolog 3 (TNFRH3; also tumor necrosis factor receptor superfamily member 26) is a 21 kDa (predicted, unglycosylated) type I transmembrane protein and member of the TNF receptor superfamily. This gene is mapped to 7 F5; 7 88.26 cM. The ECD contains two potential sites of N-linked glycosylation and a cysteine-rich region (aa 38‑126). There is no human ortholog for TNFRH3. TNFRH3 is expressed in thymus and spleen with detectable levels in the lung.

Kit Components

Catalog Number Description Quantity
EK2009-CAP Anti-Mouse Tnfrsf26 Pre-coated 96-well strip microplate 1
EK2009-ST Mouse Tnfrsf26 Standard 2 vials, 10 ng/tube
EK2009-DA Mouse Tnfrsf26 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)015.631.262.51252505001000
O.D.0.0420.1000.1550.2740.4720.8741.5432.576

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect Tnfrsf26, Dilution ratio of 1:1, concentration in serum is NO-10pg/ml.

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Mouse TNFRH3/TNFRSF26 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)2811246030118473
Standard deviation1.124.8218.861.686.8434.53
CV (%)4.0%4.3%4.1%5.6%5.8%7.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of Tnfrsf26 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 128272527261.084.1%
Sample 2112971011131056.906.5%
Sample 346045948542845820.214.4%
*number of samples for each test n=16.

Gene/Protein Information For Tnfrsf26 (Source: Uniprot.Org, NCBI)

Uniprot ID

P83626

Gene ID

244237

Gene Name

Tnfrsf26

Full Name

Tumor necrosis factor receptor superfamily member 26

Weight

22.708kDa

Alternative Names

Tumor necrosis factor receptor superfamily member 26

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Tnfrsf26, check out the Tnfrsf26 Infographic

Tnfrsf26 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Tnfrsf26: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

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7 Customer Q&As for Mouse TNFRH3/TNFRSF26 ELISA Kit PicoKine®

Question

Q: how do I analyze ELISA data? I have obtained Tnfrsf26 level in plasma.

G. Williams

Verified customer

Asked: 2020-04-07

Answer

A: we recommend you this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-04-07

Question

Q: we need your recommendation regarding the dilution ratio of serum samples for detection of Tnfrsf26 in Mouse plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have low serum quantitys so we like to dilute as much as possible.

S. Collins

Verified customer

Asked: 2019-04-04

Answer

A: without having an understanding the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the Tnfrsf26 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2019-04-04

Question

Q: how long can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring Tnfrsf26 using the EK2009 Mouse Tnfrsf26 Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2018-05-09

Answer

A: Boster Bio does not evaluate sample stability. Variations in sample collection, processing, and storage may change the stabilityof samples. It is recommend to assay sample immediately after collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to prevent protein degradation.

Boster Scientific Support

Answered: 2018-05-09

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2018-01-16

Answer

A: in those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are present in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2018-01-16

Question

Q: What is the optimal O.D. value for Tnfrsf26 ELISA kit? I used your Tnfrsf26 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain Tnfrsf26 even though the O.D. values are not very high?

Verified Customer

Verified customer

Asked: 2017-10-02

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this Tnfrsf26 ELISA kit to have sensitivity of 10pg/ml, that means the minimum amount of Tnfrsf26 that can be declared/interpreted as positive by the above standard is 10pg/ml.

Boster Scientific Support

Answered: 2017-10-02

Question

Q: if the enzyme conjugated Tnfrsf26 antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the Tnfrsf26 antigen?

L. Jha

Verified customer

Asked: 2017-05-30

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2017-05-30

Question

Q: is it okay to use EDTA plasma as samples in Mouse Tnfrsf26 Picokine® ELISA Kit (Catalog # EK2009)?

K. Nelson

Verified customer

Asked: 2016-03-28

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of Tnfrsf26 causing degradation of its protein structure. Tnfrsf26 may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes needed for detection and block the antigen antibody reaction. We have tested the Tnfrsf26 ELISA, treating samples with a number of anticoagulants and decided that heparin, EDTA or citrate can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2016-03-28

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