Click image to see more details
Product Info Summary
| SKU: | FEK1121 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Human |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA) |
| Assay Time: | 90 Minutes |
How Quick Picokine(R) ELISA kits work: Get data in 90 minutes
Product info
Product Name
Human TNFRSF13C/BAFFR PicoKine® Quick ELISA Kit
SKU/Catalog Number
FEK1121
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Human TNFRSF13C/BAFFR PicoKine® Quick ELISA Kit (90 minutes, 96 Tests). Quantitate Human TNFRSF13C/BAFFR in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10 pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months. (Ships with gel ice, can store for up to 3 days in room temperature. Refrigerate upon receipt.)
Cite This Product
Human TNFRSF13C/BAFFR PicoKine® Quick ELISA Kit (Boster Biological Technology, Pleasanton CA, USA, Catalog # FEK1121)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: NS0; Immunogen sequence: S7-A71
Capture/Detection Antibodies
The capture antibody is polyclonal antibody from goat and the detection antibody is polyclonal antibody from goat.
Sensitivity
<10 pg/ml
Assay Range
78 pg/ml - 5,000 pg/ml
Standard Dilution Instructions
See datasheet of FEK1121 for more details
Cross Reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
FEK1121 is reactive to TNFRSF13C in Human samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA)
Application Guarantee
FEK1121 is guaranteed for ELISA in Human by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of TNFRSF13C
Tumor necrosis factor receptor superfamily member 13C also known as B-cell-activating factor receptor or BAFF receptor is a protein that in humans is encoded by the TNFRSF13C gene. By homology to a BAC clone, the BAFFR gene was mapped to chromosome 22q13.1-q13.31. B-cell activating factor(BAFF) enhances B-cell survival in vitro and is a regulator of the peripheral B-cell population. The protein encoded by this gene is a receptor for BAFF and is a type III transmembrane protein containing a single extracellular phenylalanine-rich domain. It is thought that this receptor is the principal receptor required for BAFF-mediated mature B-cell survival.
Kit Components
| Catalog number | Description | Quantity | Volume |
|---|---|---|---|
| FEK1121-CAP | Anti-tag Pre-coated 96-well Strip Microplate | 1 | 12 strips of 8 wells |
| EK1121-ST | Human TNFRSF13C Standard | 2 | 10 ng/tube |
| FEK1121-A | Human TNFRSF13C Antibody A | 1 | 3ml |
| FEK1121-B | Human TNFRSF13C Antibody B | 1 | 3ml |
| AR1106-1 | Sample Diluent | 1 | 15ml |
| AR1106-7 | TBS-T Wash Buffer (25x) | 1 | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 1 | 10ml |
| AR1105 | Stop Solution | 1 | 10ml |
| PLA-SEA | Adhesive Plate Sealers | 2 | Piece |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional)
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
- Horizontal orbital microplate shaker capable of maintaining a speed of 500 rpm, amplitude 3mm.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration(pg/ml) | 0 | 78 | 156 | 312 | 625 | 1,250 | 2,500 | 5,000 |
| O.D. | 0.031 | 0.120 | 0.192 | 0.341 | 0.604 | 1.071 | 1.875 | 2.426 |
Data Example Images
Click image to see more details
Human TNFRSF13C PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Human TNFRSF13C/BAFFR PicoKine® Quick ELISA Kit within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 179 | 685 | 2752 | 158 | 702 | 2533 |
| Standard deviation | 13.06 | 51.37 | 217.40 | 13.43 | 61.07 | 210.23 |
| CV(%) | 7.3% | 7.5% | 7.9% | 8.5% | 8.7% | 8.3% |
Protein Target Info & Infographic
Gene/Protein Information For TNFRSF13C (Source: Uniprot.Org, NCBI)
Gene Name
TNFRSF13C
Full Name
Weight
18.9 kDa
For more info on TNFRSF13C, check out the TNFRSF13C Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for TNFRSF13C: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us support@bosterbio.com.
Specific Publications For Human TNFRSF13C/BAFFR PicoKine® Quick ELISA Kit (FEK1121)
Loading publications
Recommended Resources
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Customer Reviews
0 Reviews For Human TNFRSF13C/BAFFR PicoKine® Quick ELISA Kit
Customer Q&As
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question
