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Product Info Summary
| SKU: | HSEK0398 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Mouse |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (heparin, EDTA, citrate). |
Product info
Product Name
Mouse IL-2 High Sensitivity ELISA Kit PicoKine®
SKU/Catalog Number
HSEK0398
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Mouse IL-2 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Il2 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate). Sensitivity: 0.005pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Mouse IL-2 High Sensitivity ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # HSEK0398)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: A21-Q169
Sensitivity
<0.005 pg/ml
Assay Range
0.31-20pg/ml
Standard Dilution Instructions
See datasheet of HSEK0398 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
HSEK0398 is reactive to IL2 in Mouse samples
Validated Sample Types
cell culture supernatants, serum and plasma (heparin, EDTA, citrate).
Application Guarantee
HSEK0398 is guaranteed for ELISA in Mouse by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IL2
Interleukin-2 (IL2), formerly referred to as T-cell growth factor, is a powerfully immunoregulatory lymphokine that is produced by lectin- or antigen-activated T cells. It is produced not only by mature T lymphocytes on stimulation but also constitutively by certain T-cell lymphoma cell lines. The lymphokine interleukin-2 (IL-2) is responsible for autocrine cell cycle progression and regulation of immune responses. IL-2 expression in mature thymocytes and T cells has been found to be tightly controlled by monoallelic expression. IL-2 can act as a growth hormone for both B and T lymphocytes. The human gene for interleukin 2 (IL2) is assigned to chromosome 4. Mouse IL-2 contains 149 amino acids in mature form with the molecular mass of 17.2KDa.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| HSEK0398-CAP | Anti-Mouse IL2 Pre-coated 96-well strip microplate | 1 |
| HSEK0398-ST | Mouse IL2 Standard | 2 vials, 10 ng/tube |
| HSEK0398-DA | Mouse IL2 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 0.3 | 0.63 | 1.25 | 2.5 | 5 | 10 | 20 |
| O.D. | 0.053 | 0.126 | 0.188 | 0.301 | 0.536 | 0.973 | 1.747 | 2.88 |
Data Example Images
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Mouse IL-2 High Sensitivity PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Mouse IL-2 High Sensitivity ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 1 | 2 | 8 | 1 | 2 | 5 |
| Standard deviation | 0.06 | 0.09 | 0.55 | 0.02 | 0.1 | 0.22 |
| CV (%) | 6.2% | 4.4% | 6.9% | 2.5% | 4.9% | 4.3% |
Specific Publications For Mouse IL-2 High Sensitivity ELISA Kit PicoKine® (HSEK0398)
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