Total Protein Analysis



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  1. BCA Protein Assay Kit AR0146-1L
    $400.00
    • Western Blotting Reagents, Total Protein Analysis
    • 1kit
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    BCA Protein Assay Kit, Western Blotting Related Reagent Learn More
  2. BCA Protein Assay Kit AR0146-500
    $230.00
    • Western Blotting Reagents, Total Protein Analysis
    • 1kit
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    BCA Protein Assay Kit, Western Blotting Related Reagent Learn More
  3. Silver Stain kit AR0171
    $100.00
    • Western Blotting Reagents, Electrophoresis/Gel Staining, Total Protein Analysis
    • 1kit
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    Silver Staining Kit uses ammoniacal silver chemistry and glutaraldehyde sensitization to produce a highly sensitive silver stain, capable of detecting much lower levels of protein than standard Coomassie or Colloidal Blue techniques. Clear background makes sample identification unambiguous and provides publication-quality gels. With the advantages of easy and effective operation, Boster's Silver Stain kit is suitable for the SDS-PAGE or non-denaturing PAGE. It is suitable for staining both single dimension SDS-PAGE gels and two-dimensional (2D) gels of complex protein solutions, and the staining gel will be compatible with subsequent mass spectrometry detection. The sensitivity of silver staining is 100 times as high as that of CBB (Coomassie brilliant blue) staining. The kit can detect 0.25 ng BSA. It is unnecessary to use the toxic methanol. Learn More
  4. Coomassie Blue Fast Staining Solution AR0170
    $30.00
    • Western Blotting Reagents, Electrophoresis/Gel Staining, Total Protein Analysis
    • 1kit
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    Boster’s Commassie Blue Fast Staining Solution is used for rapid staining of protein on polyacrylamide gels. Learn More
  5. Coomassie Blue Staining Destaining Solution AR0140
    $20.00
    • Western Blotting Reagents, Electrophoresis/Gel Staining, Total Protein Analysis
    • 1kit
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    Boster’s Commassie Blue Staining Destaining Solution is used for staining of protein on polyacrylamide gels. Learn More
  6. Micro BCA Protein Assay Kit AR1110
    $80.00
    • Western Blotting Reagents, Total Protein Analysis
    • 1kit
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    Boster’s Micro BCA Protein Assay Kit is a ready-to-use, detergent-compatible, Western blot related, total protein analysis reagent, used for the quick determination of total protein concentration of dilute protein solutions. Learn More
  7. BCA Protein Assay Kit AR0146
    $50.00
    • Western Blotting Reagents, Total Protein Analysis
    • 1kit
    • boster_tick.pngShips next business day
    BCA Protein Assay Kit, Western Blotting Related Reagent Learn More
  8. Coomassie (Bradford) Protein Assay Kit AR0145
    $20.00
    • Western Blotting Reagents, Electrophoresis/Gel Staining, Total Protein Analysis
    • 1kit
    • boster_tick.pngShips next business day
    Coomassie Plus Protein Assay Kit, Western Blotting Related Reagent Learn More

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Western Blot Protein Quantification Assays

western protein assay

Introduction

After protein sample preparation, the next step of western blotting is total protein analysis. Total protein analysis is the process of quantifying the protein concentration. Protein concentration data is critical to getting good results on your blot: equal amounts of protein must be loaded into each well of the gel to avoid saturation issues, ghost bands, and other trouble. The concentration data produced by total protein analysis can also perform as a reliable loading control for semi-quantitative western blotting. Boster provides a range of high-quality protein analysis kits so you can get more accurate protein concentration data and better blots.

Procedure

To quantify the protein using the BCA protein assay kit, the first step is to reconstitute the bovine serum albumin standard samples with 0.9% NaCl or PBS solution to create a solution with a known concentration. After preparing the BSA standard solution, create a gradient dilution using the BSA standard solution and the diluent solution included in the kit. Prepare the BCA working reagent using a 50:1 reagent A:B ratio and add it to the BSA gradient dilution. Incubate at room temperature and measure the absorbance of the samples at 562 nm. Plot the standard curve to determine the protein concentration of your protein sample.

For a detailed protocol for total protein analysis, see our Western blot handbook.

Tips

  1. Select the right protein assay for your application; choose between silver staining reagents, BCA assays, and commassie blue stains.
  2. Avoid inconsistent band strengths across wells, streaked bands, and saturation problems by getting accurate protein concentrations and optimizing loading amounts. See our troubleshooting guides for more information.