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Boster offers custom polyclonal antibody production for researchers who use non-mammalian models such as Zebrafish, Drophila, C. elegans and Yeast at $600. Contact us for a free consultation.
Boster offers high quality custom antibody production services, including Rabbit and Mouse monoclonal, as well as rabbit polyclonal. For Hu, Mo and Ra targets, we provide Immunoassay development service.For non-hu-mo-ra targets,take advantage of our $600 rare species custom polyclonal program.
Boster provides plate-based multiplex cytokine immunoassay service for analytes from human, mouse and rat. Contact us today and get a free consultation.
Boster offers custom recombinant protein expression service. Available expresssion systems include E. Coli, Yeast, Insect and Mamalian Cells. Get a free consultation today.
Boster offers custom DNA synthesis service for as low as $0.08 per bp. Any gene cloning into any vector, 100% accuracy, Fast turn around time. Get a free consultation today.
Boster offers a full range of lab services for immunohistochemistry (IHC) and immunofluorescence (IF). Get a free consultation today.
Want to have all technical references by your finger tips? Download our FREE eBooks for WB, IHC, ELISA, Flow cytometry and Molecular biology here. These ebooks contain detailed information regarding the principles, protocols troubleshooting tips and optimization tips for their respective assays. Handy for newbies and veterans alike.
Breast Cancer Regulation
After protein sample preparation, the next step of western blotting is total protein analysis. Total protein analysis is the process of quantifying the protein concentration. Protein concentration data is critical to getting good results on your blot: equal amounts of protein must be loaded into each well of the gel to avoid saturation issues, ghost bands, and other trouble. The concentration data produced by total protein analysis can also perform as a reliable loading control for semi-quantitative western blotting. Boster provides a range of high-quality protein analysis kits so you can get more accurate protein concentration data and better blots.
To quantify the protein using the BCA protein assay kit, the first step is to reconstitute the bovine serum albumin standard samples with 0.9% NaCl or PBS solution to create a solution with a known concentration. After preparing the BSA standard solution, create a gradient dilution using the BSA standard solution and the diluent solution included in the kit. Prepare the BCA working reagent using a 50:1 reagent A:B ratio and add it to the BSA gradient dilution. Incubate at room temperature and measure the absorbance of the samples at 562 nm. Plot the standard curve to determine the protein concentration of your protein sample.
For a detailed protocol for total protein analysis, see our Western blot handbook.
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