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Rat CCL4/MIP-1 Beta ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ccl4 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).. Sensitivity: 10pg/ml. Cited in 1 publication(s).
|Size:||96 wells/kit, with removable strips.|
|Sample Type:||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
|Sample Volume:||100μl per well|
|Assay Range:||15.6 pg/ml - 1,000 pg/ml|
|Product Name||Rat CCL4/MIP-1 Beta ELISA Kit PicoKine™
See more CCL4/MIP-1 beta products
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Rat CCL4/MIP-1 Beta ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ccl4 in cell culture supernatants, serum and plasma (heparin, EDTA, citrate).. Sensitivity: 10pg/ml.|
|Cite This Product||Rat CCL4/MIP-1 Beta ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0939)|
|ELISA Validation||ELISA Validation Information|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernatants, serum and plasma (heparin, EDTA, citrate).|
|Assay Range||15.6 pg/ml - 1,000 pg/ml|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: A24-N92|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96 wells/kit, with removable strips.|
|NCBI Gene Id||116637|
|Species Of This Entry||Rat|
|Protein Name||C-C motif chemokine 4|
|Superfamily||intercrine beta (chemokine CC) family|
|Alternative Names||CCL4/MIP-1 beta|ACT2; ACT-2; AT744.1; C-C motif chemokine 4; CCL4; chemokine (C-C motif) ligand 4; Exodus-3; G-26 T-lymphocyte-secreted protein; G-26; HC21; LAG1; LAG-1; Lymphocyte activation gene 1 protein; Macrophage inflammatory protein 1-beta; MIP1 beta; MIP-1 beta; MIP1B; MIP1B1; MIP-1-beta; MIP-1-beta(1-69); PAT 744; Protein H400; SCYA2; SCYA4; secreted protein G-26; SIS-gamma; small inducible cytokine A4 (homologous to mouse Mip-1b); Small-inducible cytokine A4; T-cell activation protein 2|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Ccl4: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0939-CAP||Anti-Rat Ccl4 Pre-coated 96-well strip microplate||1|
|EK0939-ST||Rat Ccl4 Standard||2 vials, 10 ng/tube|
|EK0939-DA||Rat Ccl4 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
This data is generated from a recent batch of EK0939 Rat CCL4/MIP-1 Beta ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Rat CCL4/MIP-1 Beta ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Rat CCL4/MIP-1 Beta ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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|Ying-nan Ju,Qi-hang Tai,Guang-xiao Xu,Xiao-qing Zhao,Hai-bin Sun,Wei Gao,"Diannexin Can Ameliorate Acute Respiratory Distress Syndrome in Rats by Promoting Heme Oxygenase-1 Expression",Mediators of Inflammation,vol.2021,Article ID 1946384,10 pages,2021.https://doi.org/10.1155/2021/1946384
EK0939 usage in article: APP:ELISA, SAMPLE:BALF AND SERUM, DILUTION:NA
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Comprehensive info on Ccl4, beautifully presented.Open Infographic
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