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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Rat FGF1/Fgf Acidic ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat FGF1/Fgf Acidic ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Fgf1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Rat FGF1/Fgf Acidic ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK1173)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: F16-D155
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK1173 is reactive to Fgf1 in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK1173 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK1173-CAP||Anti-Rat Fgf1 Pre-coated 96-well strip microplate||1|
|EK1173-ST||Rat Fgf1 Standard||2 vials, 10 ng/tube|
|EK1173-DA||Rat Fgf1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat FGF1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect FGF acidic/FGF1, Dilution ratio of 1:10, concentration in serum is around 5.6ng/ml.
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat FGF1/Fgf Acidic ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of FGF acidic/FGF1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Fgf1 (Source: Uniprot.Org, NCBI)
Fibroblast growth factor 1
heparin-binding growth factors family
AFGF; alpha; alpha-ECGF; beta-ECGF; ECGF; ECGFB; ECGF-betaAcidic fibroblast growth factor; endothelial cell growth factor, beta; FGF acidic; FGF-1; FGFABeta-endothelial cell growth factor; FGF-alpha; fibroblast growth factor 1 (acidic); GLIO703; HBGF1; HBGF-1; heparin-binding growth factor 1 Fgf1|FGF-1, Fgf2b, HBGF-1, HBGF1|fibroblast growth factor 1|fibroblast growth factor 1|Fibroblast growth factor 1 (heparin binding)|aFGF|acidic fibroblast growth factor|fibroblast growth factor 1 (acidic)|fibroblast growth factor 2b|heparin-binding growth factor 1*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Fgf1, check out the Fgf1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Fgf1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
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