Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™

Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ifng in cell culture supernatants and serum. Sensitivity: 6pg/ml. Cited in 13 publication(s).

Product Info Summary

SKU: EK0374
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants and serum.

Product Name

Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™

See all IFN-gamma products

SKU/Catalog Number

EK0374

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ifng in cell culture supernatants and serum. Sensitivity: 6pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0374)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: E23-C156

Sensitivity

<6 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0374 is reactive to Ifng in Rat samples

Validated Sample Types

cell culture supernatants and serum.

Application Guarantee

EK0374 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0374-CAP Anti-Rat Ifng Pre-coated 96-well strip microplate 1
EK0374-ST Rat Ifng Standard 2 vials, 10 ng/tube
EK0374-DA Rat Ifng Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0870.1280.1630.2410.4840.7481.3562.293

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IFN-gamma, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of IFN-gamma in samples: 25380621 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)7926584471248890
Standard deviation4.2615.3756.834.8217.3678.32
CV (%)5.4%5.8%6.9%6.8%7%8.8%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IFN-gamma in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 179808780813.23.9%
Sample 226528229629028311.644.1%
Sample 38448368578578488.951%
*number of samples for each test n=16.

Gene/Protein Information For Ifng (Source: Uniprot.Org, NCBI)

Uniprot ID

P01581

Gene ID

25712

Gene Name

Ifng

Full Name

Interferon gamma

Weight

17.918kDa

Superfamily

type II (or gamma) interferon family

Alternative Names

IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma Ifng|IFNG2, If2f|interferon gamma|interferon gamma|IFN-gamma|interferon 2f

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Ifng, check out the Ifng Infographic

Ifng infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Ifng: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0374 has been cited in 13 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Haiyang Zhang,Wenjing Jiao,Hailin Cui,Qinghong Sun,Honggang Fan,Combined exposure of alumina nanoparticles and chronic stress exacerbates hippocampal neuronal ferroptosis via activating IFN-γ/ASK1/JNK signaling pathway in rats,Journal of Hazardous Materia
Species: Rat
EK0374 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPUS, DILUTION:NA

Liu Yuan,Luo Pei,Zhang Ji-Zhong,Chen Xiao-Yi,Li Li-Min,Wu You-Jiao,Yin Qiao-Zhi,Huang Li.Identification of Active Compounds From Yi Nationality Herbal Formula Wosi Influencing COX-2 and VCAM-1 Signaling. Frontiers in Pharmacology,11.2020.1690.https://www.
Species: Rat
EK0374 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

The conditioned medium of human mesenchymal stromal cells reduces irradiation-induced damage in cardiac fibroblast cells

Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa ?-related pathway in rats

Valproic acid attenuates traumatic spinal cord injury-induced inflammation via STAT1 and NF-?B pathway dependent of HDAC3

Effects of leukotriene B4 on interleukin-32, interferon-? and chemokines in rats with rheumatoid arthritis

??TCR immunoglobulin constant region domain exchange in human ??TCRs improves TCR pairing without altering TCR gene-modified T cell function

The protective effect of the natural compound hesperetin against fulminant hepatitis in vivo and in vitro

Inhibition of myeloid differentiation factor 88 signaling mediated by histidine-grafted poly(?-amino ester) ester nanovector induces donor-specific liver allograft tolerance

Adenovirus-mediated delivery of interferon-? gene inhibits the growth of nasopharyngeal carcinoma

Chen X, Wu S, Chen C, Xie B, Fang Z, Hu W, Chen J, Fu H, He H. J Neuroinflammation. 2017 Jul 24;14(1):143. doi: 10.1186/s12974-017-0917-3. Omega-3 polyunsaturated fatty acid supplementation attenuates microglial-induced inflammation by inhibiting ...

Sofian M, Aghakhani A, Farazi Aa, Banifazl M, Eslamifar A, Rashidi N, Khadem Sadegh A, Ramezani A. Hepat Mon. 2012 Dec;12(12):E6156. Doi: 10.5812/Hepatmon.6156. Epub 2012 Dec 29. Serum Profile Of T Helper 1 And T Helper 2 Cytokines In Hepatitis C ...

Pan Zy, Wang H. Int Immunopharmacol. 2014 May;20(1):229-37. Doi: 10.1016/J.Intimp.2014.03.004. Epub 2014 Mar 19. Synergistic Interaction Between Choline And Aspirin Against Acute Inflammation Induced By Carrageenan And Lipopolysaccharide.

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5 Customer Q&As for Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™

Question

Q: how to analyze ELISA data? I measured IFN-gamma level in serum.

Verified Customer

Verified customer

Asked: 2020-08-10

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. we also provide a convenient online tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2020-08-10

Question

Q: for how much duration can samples (cell cultures, serum, and plasma) be stored and still be stable for measuring IFN-gamma using the EK0374 Rat IFN-gamma Picokine® ELISA Kit?

Verified Customer

Verified customer

Asked: 2019-07-30

Answer

A: Boster Bio does not assess sample stability. Variations in sample collection, processing, and storage may affect the stabilityof samples. It is recommend to assay sample on-stat collection when possible, or aliquot into single use volumes and store samples frozen. avoid repeat freeze-thaw cycles with the stored samples to limit protein degradation.

Boster Scientific Support

Answered: 2019-07-30

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

B. Zhou

Verified customer

Asked: 2018-02-16

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2018-02-16

Question

Q: can you suggest the dilution ratio of serum samples for detection of IFN-gamma in Rat serum? I am trying to measure a multiple analytes and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

S. Davis

Verified customer

Asked: 2014-10-28

Answer

A: without knowing the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IFN-gamma ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2014-10-28

Question

Q: if the enzyme conjugated IFN-gamma antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IFN-gamma antigen?

Verified Customer

Verified customer

Asked: 2014-03-16

Answer

A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.

Boster Scientific Support

Answered: 2014-03-16

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