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Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ifng in cell culture supernatants and serum.. Sensitivity: 6pg/ml. Cited in 13 publication(s).
|Size:||96 wells/kit, with removable strips.|
|Sample Type:||cell culture supernatants and serum.|
|Sample Volume:||100μl per well|
|Assay Range:||31.2 pg/ml - 2,000 pg/ml|
|Product Name||Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™
See more IFN-gamma products
|Storage & Handling||Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)|
|Description||Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ifng in cell culture supernatants and serum.. Sensitivity: 6pg/ml.|
|Cite This Product||Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0374)|
|ELISA Validation||ELISA Validation Information|
*Our Boster Guarantee covers the use of this product in the above tested applications.
**For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab.
|Sample Type||cell culture supernatants and serum.|
|Assay Range||31.2 pg/ml - 2,000 pg/ml|
|Immunogen/Standard||Expression system for standard: E.coli; Immunogen sequence: E23-C156|
|Antibody Clonalities||Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat
|Cross Reactivity||There is no detectable cross-reactivity with other relevant proteins.|
|Pack Size||96 wells/kit, with removable strips.|
|NCBI Gene Id||25712|
|Species Of This Entry||Rat|
|Protein Name||Interferon gamma|
|Superfamily||type II (or gamma) interferon family|
|Alternative Names||IFN-gamma|IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Want a nice infographic on your next protein? Boster's got them all. All proteins in the human genome, and some, can be found in the Boster Bio gene infographics. Download it and share it with your colleagues and friends. Big size posters available upon request.
In this infographic you will see the following information for Ifng: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. Some times it even contains some opt-ed articles the Boster team on the scientific news and clinical impacts of this protein, though not all have that. Too many proteins, you know.
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
|EK0374-CAP||Anti-Rat Ifng Pre-coated 96-well strip microplate||1|
|EK0374-ST||Rat Ifng Standard||2 vials, 10 ng/tube|
|EK0374-DA||Rat Ifng Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
This data is generated from a recent batch of EK0374 Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
We measured random samples of Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We measured the performance consistency of Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
Here are featured tools and databases that you might find useful.
|Haiyang Zhang,Wenjing Jiao,Hailin Cui,Qinghong Sun,Honggang Fan,Combined exposure of alumina nanoparticles and chronic stress exacerbates hippocampal neuronal ferroptosis via activating IFN-γ/ASK1/JNK signaling pathway in rats,Journal of Hazardous Materia
EK0374 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPUS, DILUTION:NA
|Liu Yuan,Luo Pei,Zhang Ji-Zhong,Chen Xiao-Yi,Li Li-Min,Wu You-Jiao,Yin Qiao-Zhi,Huang Li.Identification of Active Compounds From Yi Nationality Herbal Formula Wosi Influencing COX-2 and VCAM-1 Signaling. Frontiers in Pharmacology,11.2020.1690.https://www.
EK0374 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
|The conditioned medium of human mesenchymal stromal cells reduces irradiation-induced damage in cardiac fibroblast cells||PubMed ID 30010837|
|Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa ?-related pathway in rats||PubMed ID 29928110|
|Valproic acid attenuates traumatic spinal cord injury-induced inflammation via STAT1 and NF-?B pathway dependent of HDAC3||PubMed ID 29776446|
|Effects of leukotriene B4 on interleukin-32, interferon-? and chemokines in rats with rheumatoid arthritis||PubMed ID 28912850|
|??TCR immunoglobulin constant region domain exchange in human ??TCRs improves TCR pairing without altering TCR gene-modified T cell function||PubMed ID 28259946|
|The protective effect of the natural compound hesperetin against fulminant hepatitis in vivo and in vitro||PubMed ID 27714757|
|Inhibition of myeloid differentiation factor 88 signaling mediated by histidine-grafted poly(?-amino ester) ester nanovector induces donor-specific liver allograft tolerance||PubMed ID 26185440|
|Adenovirus-mediated delivery of interferon-? gene inhibits the growth of nasopharyngeal carcinoma||PubMed ID 23272637|
|Chen X, Wu S, Chen C, Xie B, Fang Z, Hu W, Chen J, Fu H, He H. J Neuroinflammation. 2017 Jul 24;14(1):143. doi: 10.1186/s12974-017-0917-3. Omega-3 polyunsaturated fatty acid supplementation attenuates microglial-induced inflammation by inhibiting ...||PubMed ID 28738820|
|Sofian M, Aghakhani A, Farazi Aa, Banifazl M, Eslamifar A, Rashidi N, Khadem Sadegh A, Ramezani A. Hepat Mon. 2012 Dec;12(12):E6156. Doi: 10.5812/Hepatmon.6156. Epub 2012 Dec 29. Serum Profile Of T Helper 1 And T Helper 2 Cytokines In Hepatitis C ...||PubMed ID 23423691|
|Pan Zy, Wang H. Int Immunopharmacol. 2014 May;20(1):229-37. Doi: 10.1016/J.Intimp.2014.03.004. Epub 2014 Mar 19. Synergistic Interaction Between Choline And Aspirin Against Acute Inflammation Induced By Carrageenan And Lipopolysaccharide.||PubMed ID 24656779|
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Comprehensive info on Ifng, beautifully presented.Open Infographic
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