Click image to see more details
Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants and serum.|
Customers Who Bought This Also Bought
Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Ifng in cell culture supernatants and serum. Sensitivity: 6pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0374)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: E23-C156
31.2 pg/ml - 2,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0374 is reactive to Ifng in Rat samples
Validated Sample Types
cell culture supernatants and serum.
EK0374 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0374-CAP||Anti-Rat Ifng Pre-coated 96-well strip microplate||1|
|EK0374-ST||Rat Ifng Standard||2 vials, 10 ng/tube|
|EK0374-DA||Rat Ifng Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
Submit A Review
Validation Standard Curve O.D. At 450nm
Data Example Images
Click image to see more details
Rat IFN gamma PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IFN-gamma, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..
Some articles we found to cite concentrations of IFN-gamma in samples: 25380621 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of IFN-gamma in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Ifng (Source: Uniprot.Org, NCBI)
type II (or gamma) interferon family
IFG; IFI; IFNG; IFNgamma; IFN-gamma; Immune interferon; interferon gamma; interferon, gamma Ifng|IFNG2, If2f|interferon gamma|interferon gamma|IFN-gamma|interferon 2f*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Ifng, check out the Ifng Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Ifng: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0374 has been cited in 13 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Haiyang Zhang,Wenjing Jiao,Hailin Cui,Qinghong Sun,Honggang Fan,Combined exposure of alumina nanoparticles and chronic stress exacerbates hippocampal neuronal ferroptosis via activating IFN-γ/ASK1/JNK signaling pathway in rats,Journal of Hazardous Materia
EK0374 usage in article: APP:ELISA, SAMPLE:HIPPOCAMPUS, DILUTION:NA
Liu Yuan,Luo Pei,Zhang Ji-Zhong,Chen Xiao-Yi,Li Li-Min,Wu You-Jiao,Yin Qiao-Zhi,Huang Li.Identification of Active Compounds From Yi Nationality Herbal Formula Wosi Influencing COX-2 and VCAM-1 Signaling. Frontiers in Pharmacology,11.2020.1690.https://www.
EK0374 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA
The conditioned medium of human mesenchymal stromal cells reduces irradiation-induced damage in cardiac fibroblast cells
Theaflavin alleviates inflammatory response and brain injury induced by cerebral hemorrhage via inhibiting the nuclear transcription factor kappa ?-related pathway in rats
Valproic acid attenuates traumatic spinal cord injury-induced inflammation via STAT1 and NF-?B pathway dependent of HDAC3
Effects of leukotriene B4 on interleukin-32, interferon-? and chemokines in rats with rheumatoid arthritis
??TCR immunoglobulin constant region domain exchange in human ??TCRs improves TCR pairing without altering TCR gene-modified T cell function
The protective effect of the natural compound hesperetin against fulminant hepatitis in vivo and in vitro
Inhibition of myeloid differentiation factor 88 signaling mediated by histidine-grafted poly(?-amino ester) ester nanovector induces donor-specific liver allograft tolerance
Adenovirus-mediated delivery of interferon-? gene inhibits the growth of nasopharyngeal carcinoma
Chen X, Wu S, Chen C, Xie B, Fang Z, Hu W, Chen J, Fu H, He H. J Neuroinflammation. 2017 Jul 24;14(1):143. doi: 10.1186/s12974-017-0917-3. Omega-3 polyunsaturated fatty acid supplementation attenuates microglial-induced inflammation by inhibiting ...
Sofian M, Aghakhani A, Farazi Aa, Banifazl M, Eslamifar A, Rashidi N, Khadem Sadegh A, Ramezani A. Hepat Mon. 2012 Dec;12(12):E6156. Doi: 10.5812/Hepatmon.6156. Epub 2012 Dec 29. Serum Profile Of T Helper 1 And T Helper 2 Cytokines In Hepatitis C ...
Pan Zy, Wang H. Int Immunopharmacol. 2014 May;20(1):229-37. Doi: 10.1016/J.Intimp.2014.03.004. Epub 2014 Mar 19. Synergistic Interaction Between Choline And Aspirin Against Acute Inflammation Induced By Carrageenan And Lipopolysaccharide.
Here are featured tools and databases that you might find useful.
- Boster's Pathways Library
- Protein Databases
- Bioscience Research Protocol Resources
- Data Processing & Analysis Software
- Photo Editing Software
- Scientific Literature Resources
- Research Paper Management Tools
- Molecular Biology Software
- Primer Design Tools
- Bioinformatics Tools
- Phylogenetic Tree Analysis
Have you used Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™?
Submit a review and receive an Amazon gift card.
- $25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image
- $10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image
Submit A Review
Be the first to review Rat IFN Gamma/IFNG/Interferon Gamma ELISA Kit PicoKine™
*The first user to submit a review for a product is eligible for Boster's Innovating Scientists Reward, which gives a free antibody. This is in addition to the gift card reward.
Have a question?
Find answers in Q&As, reviews.
Can't find your answer?
Submit your question