Rat IGF-1 ELISA Kit PicoKine™

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EK0377 IGF-1 ELISA Kit (Rat) detects free IGF-1.

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A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

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A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

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A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IGF-I causing disruption of its protein structure. IGF-I may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IGF-I ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

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A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

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A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IGF-I ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of IGF-I that can be declared/interpreted as positive by the above standard is 5pg/ml.

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A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

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A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IGF-I for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

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The standard of the Rat IGF-1 ELISA Kit PicoKine™ EK0377 is recombinant and the expression system is E. coli.

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For the Rat IGF-1 ELISA Kit PicoKine™ EK0377, there wasn't any virus vector used in the recombination and/or expression process.

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The standard of the Rat IGF-1 ELISA Kit PicoKine™ EK0377 contains 0.02% sodium azide.

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The Stop Solution for the Rat IGF-1 ELISA Kit PicoKine™ EK0377 is 2% Sulphuric acid.

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The Rat IGF-1 ELISA Kit PicoKine™ EK0377 contains sodium azide (0.02%), sulphuric acid (2%) and proclin(0.2%).

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There isn't any wash step after sample incubation and before antibody incubation. Our lab technicains found there wasn't any significant difference in result valus between washing and without washing the plate after sample incubation and before antibody incubation.

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The Rat IGF-1 ELISA Kit PicoKine™ (EK0377) detects free IGF-1. Usually, if the ELISA kit is not specific, it would detect total protein.