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Rat IGF-1 ELISA Kit PicoKine™
Sandwich High Sensitivity ELISA kit for Quantitative Detection of Rat IGF-1. 96wells/kit, with removable strips. Cited in 22 publication(s).
Rat IGF-1 ELISA Kit PicoKine™ Info At A Glance
| SKU: | EK0377 |
|---|---|
| Size: | 96wells/kit, with removable strips. |
| Reactivity: | Rat |
| Sample Type: | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sample Volume: | 100μl per well |
| Sensitivity: | <5pg/ml |
| Assay Range: | 62.5pg/ml-4000pg/ml |
Overview
| Product Name | Rat IGF-1 ELISA Kit PicoKine™
See all Igf1 primary antibodies, ELISA kits and proteins |
|---|---|
| SKU/Catalog Number | EK0377 |
| Storage & Handling | Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description | Detect Rat Igf-I/IGF1 with <5pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Igf-I/IGF1 tissue specificity: |
| Cite This Product | Rat IGF-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0377) |
Product Specs
| Reactivity/Species | Rat |
|---|---|
| Applications | ELISA
*Our Boster Guarantee covers the use of this product in the above tested applications. **For positive and negative control design, consult "Tissue specificity" under "Protein Target Info" tab. |
| Sample Type | cell culture supernates, cell lysates, serum and plasma (heparin, EDTA). |
| Sensitivity | <5pg/ml |
| Assay Range | 62.5pg/ml-4000pg/ml |
| Immunogen | Expression system for standard: E.coli; Immunogen sequence: G49-A118 |
| Antibody Clonalities | Capture antibody|Detection antibody monoclonal antibody from mouse|polyclonal antibody from goat specific for IGF-1 |
| Cross Reactivity | There is detectable cross-reactivity with IGF-2< 1%. |
| Pack Size | 96wells/kit, with removable strips. |
Protein Target Info (Source: Uniprot.org)
| Uniprot Id | P08025 |
|---|---|
| Gene Name | Igf1 |
| Protein Name | Insulin-like growth factor I |
| Subcellular Localization | Secreted. |
*if product is indicated to react with multiple species, protein info is based on the human gene.
Ontology
| Protein Function | The insulin-like growth factors, isolated from plasma, are structurally and functionally related to insulin but have a much higher growth-promoting activity. May be a physiological regulator of [1-14C]-2-deoxy-D-glucose (2DG) transport and glycogen synthesis in osteoblasts. Stimulates glucose transport in rat bone-derived osteoblastic (PyMS) cells and is effective at much lower concentrations than insulin, not only regarding glycogen and DNA synthesis but also with regard to enhancing glucose uptake. May play a role in synapse maturation (By similarity). . |
|---|---|
| Background | Insulin decreases blood glucose concentration. It increases cell permeability to monosaccharides, amino acids and fatty acids. It accelerates glycolysis, the pentose phosphate cycle, and glycogen synthesis in liver. Functions as a hormone. |
Kit Components And Assay QC Data Details Of Rat IGF-1 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
|---|---|---|
| EK0377-CAP | 96-well plate precoated with anti-Rat Igf1 antibody | 1 |
| EK0377-ST | lyophilized recombinant Rat Igf1 standard | 10ng/tube |
| EK0377-DA | biotinylated anti-Rat Igf1 antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0377 Rat IGF-1 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.023 | 0.080 | 0.141 | 0.258 | 0.609 | 1.233 | 1.634 | 2.085 |
We measured random samples of Rat IGF-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 185 | 754 | 1874 | 186 | 764 | 1861 |
| Standard deviation | 9.99 | 56.55 | 80.58 | 11.16 | 69.52 | 94.91 |
| CV(%) | 5.4% | 7.5% | 4.3% | 6% | 9.1% | 5.1% |
We measured the performance consistency of Rat IGF-1 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 185 | 177 | 173 | 194 | 182 | 8.04 | 4.4% |
| Sample 2 | 754 | 688 | 705 | 708 | 713 | 24.45 | 3.4% |
| Sample 3 | 1874 | 1778 | 1907 | 1705 | 1816 | 79.7 | 4.3% |
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
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Total number of citations: 22
| Baykara B, Aksu I, Buyuk E, Kiray M, Sisman Ar, Baykara B, Dayi A, Tas A, Ozdemir D, Arda Mn, Uysal N. Biotech Histochem. 2013 Jul;88(5):250-7. Doi: 10.3109/10520295.2013.769630. Epub 2013 Mar 13. Progesterone Treatment Decreases Traumatic Brain I... | PubMed ID 23480228 |
| Ozdemir D, Aksu I, Baykara B, Ates M, Sisman Ar, Kiray M, Buyuk A, Uysal N. Toxicol Ind Health. 2016 Jan;32(1):39-46. Doi: 10.1177/0748233713498439. Epub 2013 Aug 1. Effects Of Administration Of Subtoxic Doses Of Acetaminophen On Liver And Blood L... | PubMed ID 23907830 |
| Aksu I, Baykara B, Kiray M, Gurpinar T, Sisman Ar, Ekerbicer N, Tas A, Gokdemir-Yazar O, Uysal N. Biotech Histochem. 2013 May;88(3-4):194-201. Doi: 10.3109/10520295.2012.758311. Epub 2013 Jan 21. Serum Igf-1 Levels Correlate Negatively To Liver Da... | PubMed ID 23331186 |
| Adise S, Saliu A, Maldonado N, Khatri V, Cardoso L, Rodr??guez-Contreras A. J Neurosci. 2014 Mar 26;34(13):4528-33. Doi: 10.1523/Jneurosci.4188-13.2014. Effect Of Maternal Care On Hearing Onset Induced By Developmental Changes In The Auditory Peri... | PubMed ID 24671998 |
| Li M, Peng J, Song Y, Liang H, Mei Y, Fang Y. J Huazhong Univ Sci Technolog Med Sci. 2012 Oct;32(5):746-9. Doi: 10.1007/S11596-012-1028-0. Epub 2012 Oct 18. Electro-Acupuncture Combined With Transcranial Magnetic Stimulation Improves Learning And ... | PubMed ID 23073807 |
| Cai B, Li X, Wang Y, Liu Y, Yang F, Chen H, Yin K, Tan X, Zhu J, Pan Z, Wang B, Lu Y. PLoS One. 2013 May 7;8(5):e63561. doi: 10.1371/journal.pone.0063561. Print 2013. Apoptosis of bone marrow mesenchymal stem cells caused by homocysteine via acti... | PubMed ID 23667638 |
| Yang H, Wu S, Feng R, Huang J, Liu L, Liu F, Chen Y. Stem Cell Res Ther. 2017 Nov 21;8(1):267. doi: 10.1186/s13287-017-0718-8. Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats | PubMed ID 29157289 |
| Regulation of signaling pathways downstream of IGF-I/insulin by androgen in skeletal muscle of glucocorticoid-treated rats | PubMed ID 4085982 |
| Heshouwu decoction, a Chinese herb for tonifying kidney, ameliorates hypothalamic-pituitary- testicular axis secretion in aging rats | PubMed ID 25657700 |
| Mesenchymal Stem Cells and Cardiomyocytes Interplay to Prevent Myocardial Hypertrophy | PubMed ID 26586774 |
| Let-7a inhibits migration of melanoma cells via down-regulation of HMGA2 expression | PubMed ID 27725848 |
| Relationship between circulating IGF-1 levels and traumatic brain injury-induced hippocampal damage and cognitive dysfunction in immature rats | PubMed ID 22172933 |
| Anxiety correlates to decreased blood and prefrontal cortex IGF-1 levels in streptozotocin induced diabetes | PubMed ID 23123774 |
| Positive effects of aerobic exercise on learning and memory functioning, which correlate with hippocampal IGF-1 increase in adolescent rats | PubMed ID 23792196 |
| Exercise increases leptin levels correlated with IGF-1 in hippocampus and prefrontal cortex of adolescent male and female rats | PubMed ID 28179125 |
| Effect of the IGF-1/PTEN/Akt/FoxO signaling pathway on the development and healing of water immersion and restraint stress-induced gastric ulcers in rats | PubMed ID 22735908 |
| Effects of the IGF-1/PTEN/Akt/FoxO signaling pathway on male reproduction in rats subjected to water immersion and restraint stress | PubMed ID 27779666 |
| Effects of exercise and poor indoor air quality on learning, memory and blood IGF-1 in adolescent mice | PubMed ID 24050191 |
| The levels of Pdx1/insulin, Cacna1c and Cacna1d, and β-cell mass in a rat model of intrauterine undernutrition | PubMed ID 20873977 |
| Influence of GABA on brain protein synthesis mediated by the mammalian target on the rapamycin pathway | PubMed ID 23470740 |
| Effects of carbon dioxide exposure on early brain development in rats | PubMed ID 24476563 |
| Bone marrow mesenchymal stem cells upregulate transient outward potassium currents in postnatal rat ventricular myocytes | PubMed ID 19285983 |
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Domestically in USA and Canada, ships next business day if available in stock.
Note: sample size takes longer to process, average 5-7 days.
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| (888) 466-3604 |
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