Rat IGF-1 ELISA Kit PicoKine™

Rat IGF-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Igf1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5 pg/ml. Cited in 22 publication(s).

Product Info Summary

SKU: EK0377
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Product Name

Rat IGF-1 ELISA Kit PicoKine™

See all IGF-I/IGF-1 products

SKU/Catalog Number

EK0377

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat IGF-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Igf1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5 pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat IGF-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0377)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: G49-A118

Sensitivity

<5 pg/ml

Assay Range

62.5 pg/ml - 4,000 pg/ml

Cross Reactivity

There is detectable cross-reactivity with IGF-2< 1%.

Reactive Species

EK0377 is reactive to Igf1 in Rat samples

Validated Sample Types

cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).

Application Guarantee

EK0377 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0377-CAP Anti-Rat Igf1 Pre-coated 96-well strip microplate 1
EK0377-ST Rat Igf1 Standard 2 vials, 10 ng/tube
EK0377-DA Rat Igf1 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)062.5125250500100020004000
O.D.0.0230.0800.1410.2580.6091.1331.5341.985

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IGF-I/IGF-1, Dilution ratio of 1:500, concentration in serum is around 142-340ng/ml.

Some articles we found to cite concentrations of IGF-I/IGF-1 in samples: 25558384 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat IGF-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)18575418741867641861
Standard deviation9.9956.5580.5811.1669.5294.91
CV (%)5.4%7.5%4.3%6%9.1%5.1%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IGF-I/IGF-1 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 11851771731941828.044.4%
Sample 275468870570871324.453.4%
Sample 31874177819071705181679.74.3%
*number of samples for each test n=16.

Gene/Protein Information For Igf1 (Source: Uniprot.Org, NCBI)

Uniprot ID

P08025

Gene ID

24482

Gene Name

Igf1

Full Name

Insulin-like growth factor I

Weight

17.079kDa

Superfamily

insulin family

Alternative Names

IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C Igf1|IGF|insulin-like growth factor 1|insulin-like growth factor I|IGF-I|somatomedin

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Igf1, check out the Igf1 Infographic

Igf1 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Igf1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0377 has been cited in 22 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Zheng PP, Zhang LN,Zhang J,Chang XM,Ding S,Xiao F,Guo LX.Evaluating the Effects of Different Sleep Supplement Modes in Attenuating Metabolic Consequences of Night Shift Work Using Rat Model. Nat Sci Sleep. 2020 Nov 20;12:1053-1065.doi:10.2147/NSS.S271318.
Species: Rat
EK0377 usage in article: APP:ELISA, SAMPLE:BLOOD, DILUTION:NA

Study of the effects of cyclooxygenase-2 inhibitor on the promotion of hepatic tumorigenesis in rats fed a high fat diet

Bone marrow mesenchymal stem cells upregulate transient outward potassium currents in postnatal rat ventricular myocytes

Effects of carbon dioxide exposure on early brain development in rats

Influence of GABA on brain protein synthesis mediated by the mammalian target on the rapamycin pathway

The levels of Pdx1/insulin, Cacna1c and Cacna1d, and β-cell mass in a rat model of intrauterine undernutrition

Effects of exercise and poor indoor air quality on learning, memory and blood IGF-1 in adolescent mice

Exercise increases leptin levels correlated with IGF-1 in hippocampus and prefrontal cortex of adolescent male and female rats

Positive effects of aerobic exercise on learning and memory functioning, which correlate with hippocampal IGF-1 increase in adolescent rats

Anxiety correlates to decreased blood and prefrontal cortex IGF-1 levels in streptozotocin induced diabetes

Relationship between circulating IGF-1 levels and traumatic brain injury-induced hippocampal damage and cognitive dysfunction in immature rats

Let-7a inhibits migration of melanoma cells via down-regulation of HMGA2 expression

Mesenchymal Stem Cells and Cardiomyocytes Interplay to Prevent Myocardial Hypertrophy

Heshouwu decoction, a Chinese herb for tonifying kidney, ameliorates hypothalamic-pituitary- testicular axis secretion in aging rats

Regulation of signaling pathways downstream of IGF-I/insulin by androgen in skeletal muscle of glucocorticoid-treated rats

Yang H, Wu S, Feng R, Huang J, Liu L, Liu F, Chen Y. Stem Cell Res Ther. 2017 Nov 21;8(1):267. doi: 10.1186/s13287-017-0718-8. Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats

Cai B, Li X, Wang Y, Liu Y, Yang F, Chen H, Yin K, Tan X, Zhu J, Pan Z, Wang B, Lu Y. PLoS One. 2013 May 7;8(5):e63561. doi: 10.1371/journal.pone.0063561. Print 2013. Apoptosis of bone marrow mesenchymal stem cells caused by homocysteine via acti...

Li M, Peng J, Song Y, Liang H, Mei Y, Fang Y. J Huazhong Univ Sci Technolog Med Sci. 2012 Oct;32(5):746-9. Doi: 10.1007/S11596-012-1028-0. Epub 2012 Oct 18. Electro-Acupuncture Combined With Transcranial Magnetic Stimulation Improves Learning And ...

Adise S, Saliu A, Maldonado N, Khatri V, Cardoso L, Rodr??guez-Contreras A. J Neurosci. 2014 Mar 26;34(13):4528-33. Doi: 10.1523/Jneurosci.4188-13.2014. Effect Of Maternal Care On Hearing Onset Induced By Developmental Changes In The Auditory Peri...

Aksu I, Baykara B, Kiray M, Gurpinar T, Sisman Ar, Ekerbicer N, Tas A, Gokdemir-Yazar O, Uysal N. Biotech Histochem. 2013 May;88(3-4):194-201. Doi: 10.3109/10520295.2012.758311. Epub 2013 Jan 21. Serum Igf-1 Levels Correlate Negatively To Liver Da...

Ozdemir D, Aksu I, Baykara B, Ates M, Sisman Ar, Kiray M, Buyuk A, Uysal N. Toxicol Ind Health. 2016 Jan;32(1):39-46. Doi: 10.1177/0748233713498439. Epub 2013 Aug 1. Effects Of Administration Of Subtoxic Doses Of Acetaminophen On Liver And Blood L...

Baykara B, Aksu I, Buyuk E, Kiray M, Sisman Ar, Baykara B, Dayi A, Tas A, Ozdemir D, Arda Mn, Uysal N. Biotech Histochem. 2013 Jul;88(5):250-7. Doi: 10.3109/10520295.2013.769630. Epub 2013 Mar 13. Progesterone Treatment Decreases Traumatic Brain I...

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15 Customer Q&As for Rat IGF-1 ELISA Kit PicoKine™

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-12-14

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-12-14

Question

Thank you for your assistance. We have a customer who is interested in product EK0377 IGF-1 ELISA Kit (Rat). She would like to know

Verified Customer

Verified customer

Asked: 2020-01-08

Answer

EK0377 IGF-1 ELISA Kit (Rat) detects free IGF-1.

Boster Scientific Support

Answered: 2020-01-08

Question

Are the standards for EK0377 natural material or recombinant? And if recombinant, what kind of cell or living material is used for expression?

Verified customer

Asked: 2019-12-11

Answer

The standard of the Rat IGF-1 ELISA Kit PicoKine™ EK0377 is recombinant and the expression system is E. coli.

Boster Scientific Support

Answered: 2019-12-11

Question

Was a virus vector used in the recombination and/or expression process for EK0377? What kind of virus vector is used ?

Verified customer

Asked: 2019-12-11

Answer

For the Rat IGF-1 ELISA Kit PicoKine™ EK0377, there wasn't any virus vector used in the recombination and/or expression process.

Boster Scientific Support

Answered: 2019-12-11

Question

Do the standards of EK0377 include additive agent such as antiseptic or preservative? If included, what is the composition of said additive agent?

Verified customer

Asked: 2019-12-11

Answer

The standard of the Rat IGF-1 ELISA Kit PicoKine™ EK0377 contains 0.02% sodium azide.

Boster Scientific Support

Answered: 2019-12-11

Question

Material name and concentration of Stop Solution for EK0377?

Verified customer

Asked: 2019-12-11

Answer

The Stop Solution for the Rat IGF-1 ELISA Kit PicoKine™ EK0377 is 2% Sulphuric acid.

Boster Scientific Support

Answered: 2019-12-11

Question

Does EK0377 include hazardous substance such as azide, thimerosal, 2-ME, etc?

Verified customer

Asked: 2019-12-11

Answer

The Rat IGF-1 ELISA Kit PicoKine™ EK0377 contains sodium azide (0.02%), sulphuric acid (2%) and proclin(0.2%).

Boster Scientific Support

Answered: 2019-12-11

Question

Should the plate be washed after sample incubation and before antibody incubation steps for the Rat IGF-1 ELISA Kit PicoKine?

Verified customer

Asked: 2019-11-04

Answer

There isn't any wash step after sample incubation and before antibody incubation. Our lab technicains found there wasn't any significant difference in result valus between washing and without washing the plate after sample incubation and before antibody incubation.

Boster Scientific Support

Answered: 2019-11-05

Question

Can EK0377 measure total IGF-1 or free IGF-1?

Verified customer

Asked: 2019-06-13

Answer

The Rat IGF-1 ELISA Kit PicoKine™ (EK0377) detects free IGF-1. Usually, if the ELISA kit is not specific, it would detect total protein.

Boster Scientific Support

Answered: 2019-06-14

Question

Q: how many samples can be assayed in a Picokine® ELISA Kit?

K. Wu

Verified customer

Asked: 2019-03-22

Answer

A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.

Boster Scientific Support

Answered: 2019-03-22

Question

Q: is it okay to use citrate plasma as samples in Rat IGF-I Picokine® ELISA Kit (Catalog # EK0377)?

Verified Customer

Verified customer

Asked: 2019-03-06

Answer

A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IGF-I causing disruption of its protein structure. IGF-I may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IGF-I ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.

Boster Scientific Support

Answered: 2019-03-06

Question

Q: how to analyze ELISA data? I measured IGF-I level in plasma.

Verified Customer

Verified customer

Asked: 2018-10-16

Answer

A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online

Boster Scientific Support

Answered: 2018-10-16

Question

Q: What is the optimal O.D. value for IGF-I ELISA kit? I performed your IGF-I ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IGF-I even though the O.D. values are not very high?

P. Zhang

Verified customer

Asked: 2018-02-01

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IGF-I ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of IGF-I that can be declared/interpreted as positive by the above standard is 5pg/ml.

Boster Scientific Support

Answered: 2018-02-01

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Verified Customer

Verified customer

Asked: 2016-06-26

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2016-06-26

Question

Q: how to thaw whole blood sample for IGF-I ELISA after freezing?

W. Varma

Verified customer

Asked: 2014-06-28

Answer

A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IGF-I for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.

Boster Scientific Support

Answered: 2014-06-28

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