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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Rat IGF-1 ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat IGF-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Igf1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat IGF-1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0377)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: E.coli; Immunogen sequence: G49-A118
62.5 pg/ml - 4,000 pg/ml
There is detectable cross-reactivity with IGF-2< 1%.
EK0377 is reactive to Igf1 in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0377 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0377-CAP||Anti-Rat Igf1 Pre-coated 96-well strip microplate||1|
|EK0377-ST||Rat Igf1 Standard||2 vials, 10 ng/tube|
|EK0377-DA||Rat Igf1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Innovating Scientists Reward
If you are the first to review this product, or if you have results for a special sample, species or application this product is not validated in, share your results with us and receive your next antibody/ELISA kit free of charge! Applicable to all scientists world wide.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat IGF-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IGF-I/IGF-1, Dilution ratio of 1:500, concentration in serum is around 142-340ng/ml.
Some articles we found to cite concentrations of IGF-I/IGF-1 in samples: 25558384 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat IGF-1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of IGF-I/IGF-1 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For Igf1 (Source: Uniprot.Org, NCBI)
Insulin-like growth factor I
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C Igf1|IGF|insulin-like growth factor 1|insulin-like growth factor I|IGF-I|somatomedin*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on Igf1, check out the Igf1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Igf1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0377 has been cited in 22 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zheng PP, Zhang LN,Zhang J,Chang XM,Ding S,Xiao F,Guo LX.Evaluating the Effects of Different Sleep Supplement Modes in Attenuating Metabolic Consequences of Night Shift Work Using Rat Model. Nat Sci Sleep. 2020 Nov 20;12:1053-1065.doi:10.2147/NSS.S271318.
EK0377 usage in article: APP:ELISA, SAMPLE:BLOOD, DILUTION:NA
Study of the effects of cyclooxygenase-2 inhibitor on the promotion of hepatic tumorigenesis in rats fed a high fat diet
Bone marrow mesenchymal stem cells upregulate transient outward potassium currents in postnatal rat ventricular myocytes
Effects of carbon dioxide exposure on early brain development in rats
Influence of GABA on brain protein synthesis mediated by the mammalian target on the rapamycin pathway
The levels of Pdx1/insulin, Cacna1c and Cacna1d, and β-cell mass in a rat model of intrauterine undernutrition
Effects of exercise and poor indoor air quality on learning, memory and blood IGF-1 in adolescent mice
Exercise increases leptin levels correlated with IGF-1 in hippocampus and prefrontal cortex of adolescent male and female rats
Positive effects of aerobic exercise on learning and memory functioning, which correlate with hippocampal IGF-1 increase in adolescent rats
Anxiety correlates to decreased blood and prefrontal cortex IGF-1 levels in streptozotocin induced diabetes
Relationship between circulating IGF-1 levels and traumatic brain injury-induced hippocampal damage and cognitive dysfunction in immature rats
Let-7a inhibits migration of melanoma cells via down-regulation of HMGA2 expression
Mesenchymal Stem Cells and Cardiomyocytes Interplay to Prevent Myocardial Hypertrophy
Heshouwu decoction, a Chinese herb for tonifying kidney, ameliorates hypothalamic-pituitary- testicular axis secretion in aging rats
Regulation of signaling pathways downstream of IGF-I/insulin by androgen in skeletal muscle of glucocorticoid-treated rats
Yang H, Wu S, Feng R, Huang J, Liu L, Liu F, Chen Y. Stem Cell Res Ther. 2017 Nov 21;8(1):267. doi: 10.1186/s13287-017-0718-8. Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats
Cai B, Li X, Wang Y, Liu Y, Yang F, Chen H, Yin K, Tan X, Zhu J, Pan Z, Wang B, Lu Y. PLoS One. 2013 May 7;8(5):e63561. doi: 10.1371/journal.pone.0063561. Print 2013. Apoptosis of bone marrow mesenchymal stem cells caused by homocysteine via acti...
Li M, Peng J, Song Y, Liang H, Mei Y, Fang Y. J Huazhong Univ Sci Technolog Med Sci. 2012 Oct;32(5):746-9. Doi: 10.1007/S11596-012-1028-0. Epub 2012 Oct 18. Electro-Acupuncture Combined With Transcranial Magnetic Stimulation Improves Learning And ...
Adise S, Saliu A, Maldonado N, Khatri V, Cardoso L, Rodr??guez-Contreras A. J Neurosci. 2014 Mar 26;34(13):4528-33. Doi: 10.1523/Jneurosci.4188-13.2014. Effect Of Maternal Care On Hearing Onset Induced By Developmental Changes In The Auditory Peri...
Aksu I, Baykara B, Kiray M, Gurpinar T, Sisman Ar, Ekerbicer N, Tas A, Gokdemir-Yazar O, Uysal N. Biotech Histochem. 2013 May;88(3-4):194-201. Doi: 10.3109/10520295.2012.758311. Epub 2013 Jan 21. Serum Igf-1 Levels Correlate Negatively To Liver Da...
Ozdemir D, Aksu I, Baykara B, Ates M, Sisman Ar, Kiray M, Buyuk A, Uysal N. Toxicol Ind Health. 2016 Jan;32(1):39-46. Doi: 10.1177/0748233713498439. Epub 2013 Aug 1. Effects Of Administration Of Subtoxic Doses Of Acetaminophen On Liver And Blood L...
Baykara B, Aksu I, Buyuk E, Kiray M, Sisman Ar, Baykara B, Dayi A, Tas A, Ozdemir D, Arda Mn, Uysal N. Biotech Histochem. 2013 Jul;88(5):250-7. Doi: 10.3109/10520295.2013.769630. Epub 2013 Mar 13. Progesterone Treatment Decreases Traumatic Brain I...
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