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Product Info Summary
| SKU: | EK0377 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). |
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Product info
Product Name
Rat IGF-1 ELISA Kit PicoKine®
View all IGF-I/IGF-1 ELISA kits
SKU/Catalog Number
EK0377
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat IGF-1 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Igf1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 5 pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat IGF-1 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0377)
Clonality of Antibodies
See Datasheet for details
Immunogen
Expression system for standard: E.coli; Immunogen sequence: G49-A118
Sensitivity
<5 pg/ml
Assay Range
62.5 pg/ml - 4,000 pg/ml
Cross Reactivity
There is detectable cross-reactivity with IGF-2< 1%.
Reactive Species
EK0377 is reactive to Igf1 in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
Application Guarantee
EK0377 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of IGF-I/IGF-1
Insulin-like growth factor 1 (IGF-1) that was once called somatomedin C, is a polypeptide protein hormone similar in molecular structure to insulin. It plays an important role in childhood growth and continues to have anabolic effects in adults. Human IGF1 is a single chain 70-amino acid polypeptide cross-linked by 3 disulfide bridges, with a calculated molecular mass of 7.6 kD. The IGF1 gene, mapped on 12q22-q24.1, contains 5 exons. Exons 1-4 encode the 195-amino acid precursor (IGF1B), and exons 1, 2, 3, and 5 encode the 153-residue peptide (IGF1A). The structure of IGF1 resembles that of IGF2. And the IGF1 and IGF2 genes have complex structures with multiple promoters. The expression of both genes is regulated at the levels of transcription, RNA processing, and translation. IGF-1 is produced primarily by the liver as an endocrine hormone as well as in target tissues in a paracrine/autocrine fashion. Moreover, approximately 98% of IGF-1 is always bound to one of 6 binding proteins (IGF-BP). Furthermore, IGF-1 is one of the most potent natural activators of the AKT signaling pathway, a stimulator of cell growth and multiplication and a potent inhibitor of programmed cell death.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0377-CAP | Anti-Rat Igf1 Pre-coated 96-well strip microplate | 1 |
| EK0377-ST | Rat Igf1 Standard | 2 vials, 10 ng/tube |
| EK0377-DA | Rat Igf1 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 62.5 | 125 | 250 | 500 | 1000 | 2000 | 4000 |
| O.D. | 0.023 | 0.080 | 0.141 | 0.258 | 0.609 | 1.133 | 1.534 | 1.985 |
Data Example Images
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Rat IGF-1 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect IGF-I/IGF-1, Dilution ratio of 1:500, concentration in serum is around 142-340ng/ml.
Some articles we found to cite concentrations of IGF-I/IGF-1 in samples: 25558384 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat IGF-1 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 185 | 754 | 1874 | 186 | 764 | 1861 |
| Standard deviation | 9.99 | 56.55 | 80.58 | 11.16 | 69.52 | 94.91 |
| CV (%) | 5.4% | 7.5% | 4.3% | 6% | 9.1% | 5.1% |
Reproducibility
We ensure reproducibility by testing three samples with differing concentrations of IGF-I/IGF-1 in ELISA kits from four different production batches/lots.
| Lots | Lot 1 (pg/ml) | Lot 2 (pg/ml) | Lot 3 (pg/ml) | Lot 4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|---|---|---|---|---|---|---|
| Sample 1 | 185 | 177 | 173 | 194 | 182 | 8.04 | 4.4% |
| Sample 2 | 754 | 688 | 705 | 708 | 713 | 24.45 | 3.4% |
| Sample 3 | 1874 | 1778 | 1907 | 1705 | 1816 | 79.7 | 4.3% |
Protein Target Info & Infographic
Gene/Protein Information For Igf1 (Source: Uniprot.Org, NCBI)
Uniprot ID
P08025
Gene ID
24482
Gene Name
Igf1
Full Name
Insulin-like growth factor I
Weight
17.079kDa
Superfamily
insulin family
Alternative Names
IBP1; IGF1; IGF-1; IGF1A; IGFI; IGF-I; IGF-IA; IGF-IB; insulin-like growth factor 1 (somatomedin C); insulin-like growth factor 1; insulin-like growth factor I; insulin-like growth factor IA; insulin-like growth factor IB; Mechano growth factor; MGF; Somatomedin A; Somatomedin C; somatomedin-C Igf1|IGF|insulin-like growth factor 1|insulin-like growth factor I|IGF-I|somatomedin
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".For more info on Igf1, check out the Igf1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for Igf1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
Specific Publications For Rat IGF-1 ELISA Kit PicoKine® (EK0377)
EK0377 has been cited in 22 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Zheng PP, Zhang LN,Zhang J,Chang XM,Ding S,Xiao F,Guo LX.Evaluating the Effects of Different Sleep Supplement Modes in Attenuating Metabolic Consequences of Night Shift Work Using Rat Model. Nat Sci Sleep. 2020 Nov 20;12:1053-1065.doi:10.2147/NSS.S271318.
Species: Rat
EK0377 usage in article: APP:ELISA, SAMPLE:BLOOD, DILUTION:NA
Study of the effects of cyclooxygenase-2 inhibitor on the promotion of hepatic tumorigenesis in rats fed a high fat diet
Bone marrow mesenchymal stem cells upregulate transient outward potassium currents in postnatal rat ventricular myocytes
Effects of carbon dioxide exposure on early brain development in rats
Influence of GABA on brain protein synthesis mediated by the mammalian target on the rapamycin pathway
The levels of Pdx1/insulin, Cacna1c and Cacna1d, and β-cell mass in a rat model of intrauterine undernutrition
Effects of exercise and poor indoor air quality on learning, memory and blood IGF-1 in adolescent mice
Exercise increases leptin levels correlated with IGF-1 in hippocampus and prefrontal cortex of adolescent male and female rats
Positive effects of aerobic exercise on learning and memory functioning, which correlate with hippocampal IGF-1 increase in adolescent rats
Anxiety correlates to decreased blood and prefrontal cortex IGF-1 levels in streptozotocin induced diabetes
Relationship between circulating IGF-1 levels and traumatic brain injury-induced hippocampal damage and cognitive dysfunction in immature rats
Let-7a inhibits migration of melanoma cells via down-regulation of HMGA2 expression
Mesenchymal Stem Cells and Cardiomyocytes Interplay to Prevent Myocardial Hypertrophy
Heshouwu decoction, a Chinese herb for tonifying kidney, ameliorates hypothalamic-pituitary- testicular axis secretion in aging rats
Regulation of signaling pathways downstream of IGF-I/insulin by androgen in skeletal muscle of glucocorticoid-treated rats
Yang H, Wu S, Feng R, Huang J, Liu L, Liu F, Chen Y. Stem Cell Res Ther. 2017 Nov 21;8(1):267. doi: 10.1186/s13287-017-0718-8. Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats
Cai B, Li X, Wang Y, Liu Y, Yang F, Chen H, Yin K, Tan X, Zhu J, Pan Z, Wang B, Lu Y. PLoS One. 2013 May 7;8(5):e63561. doi: 10.1371/journal.pone.0063561. Print 2013. Apoptosis of bone marrow mesenchymal stem cells caused by homocysteine via acti...
Li M, Peng J, Song Y, Liang H, Mei Y, Fang Y. J Huazhong Univ Sci Technolog Med Sci. 2012 Oct;32(5):746-9. Doi: 10.1007/S11596-012-1028-0. Epub 2012 Oct 18. Electro-Acupuncture Combined With Transcranial Magnetic Stimulation Improves Learning And ...
Adise S, Saliu A, Maldonado N, Khatri V, Cardoso L, Rodr??guez-Contreras A. J Neurosci. 2014 Mar 26;34(13):4528-33. Doi: 10.1523/Jneurosci.4188-13.2014. Effect Of Maternal Care On Hearing Onset Induced By Developmental Changes In The Auditory Peri...
Aksu I, Baykara B, Kiray M, Gurpinar T, Sisman Ar, Ekerbicer N, Tas A, Gokdemir-Yazar O, Uysal N. Biotech Histochem. 2013 May;88(3-4):194-201. Doi: 10.3109/10520295.2012.758311. Epub 2013 Jan 21. Serum Igf-1 Levels Correlate Negatively To Liver Da...
Ozdemir D, Aksu I, Baykara B, Ates M, Sisman Ar, Kiray M, Buyuk A, Uysal N. Toxicol Ind Health. 2016 Jan;32(1):39-46. Doi: 10.1177/0748233713498439. Epub 2013 Aug 1. Effects Of Administration Of Subtoxic Doses Of Acetaminophen On Liver And Blood L...
Baykara B, Aksu I, Buyuk E, Kiray M, Sisman Ar, Baykara B, Dayi A, Tas A, Ozdemir D, Arda Mn, Uysal N. Biotech Histochem. 2013 Jul;88(5):250-7. Doi: 10.3109/10520295.2013.769630. Epub 2013 Mar 13. Progesterone Treatment Decreases Traumatic Brain I...
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Customer Q&As
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Find answers in Q&As, reviews.
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22 Customer Q&As for Rat IGF-1 ELISA Kit PicoKine®
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-12-14
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-12-14
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2020-10-12
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2020-10-12
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
L. Li
Verified customer
Asked: 2020-05-01
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions
Boster Scientific Support
Answered: 2020-05-01
Question
Thank you for your assistance. We have a customer who is interested in product EK0377 IGF-1 ELISA Kit (Rat). She would like to know
Verified Customer
Verified customer
Asked: 2020-01-08
Answer
EK0377 IGF-1 ELISA Kit (Rat) detects free IGF-1.
Boster Scientific Support
Answered: 2020-01-08
Question
Q: can I use citrate plasma as samples in Chinese IGF-I Picokine® ELISA Kit (Catalog # EK0377-HA)?
P. Yang
Verified customer
Asked: 2019-12-27
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can bind metal ions from the functional domain of IGF-I causing disruption of its protein structure. IGF-I may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IGF-I ELISA, treating samples with a number of anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-12-27
Question
Are the standards for EK0377 natural material or recombinant? And if recombinant, what kind of cell or living material is used for expression?
Verified customer
Asked: 2019-12-11
Answer
The standard of the Rat IGF-1 ELISA Kit PicoKine™ EK0377 is recombinant and the expression system is E. coli.
Boster Scientific Support
Answered: 2019-12-11
Question
Was a virus vector used in the recombination and/or expression process for EK0377? What kind of virus vector is used ?
Verified customer
Asked: 2019-12-11
Answer
For the Rat IGF-1 ELISA Kit PicoKine™ EK0377, there wasn't any virus vector used in the recombination and/or expression process.
Boster Scientific Support
Answered: 2019-12-11
Question
Do the standards of EK0377 include additive agent such as antiseptic or preservative? If included, what is the composition of said additive agent?
Verified customer
Asked: 2019-12-11
Answer
The standard of the Rat IGF-1 ELISA Kit PicoKine™ EK0377 contains 0.02% sodium azide.
Boster Scientific Support
Answered: 2019-12-11
Question
Material name and concentration of Stop Solution for EK0377?
Verified customer
Asked: 2019-12-11
Answer
The Stop Solution for the Rat IGF-1 ELISA Kit PicoKine™ EK0377 is 2% Sulphuric acid.
Boster Scientific Support
Answered: 2019-12-11
Question
Does EK0377 include hazardous substance such as azide, thimerosal, 2-ME, etc?
Verified customer
Asked: 2019-12-11
Answer
The Rat IGF-1 ELISA Kit PicoKine™ EK0377 contains sodium azide (0.02%), sulphuric acid (2%) and proclin(0.2%).
Boster Scientific Support
Answered: 2019-12-11
Question
Q: which procedure should I follow in order to thaw whole blood sample for IGF-I ELISA after freezing?
B. Dhawan
Verified customer
Asked: 2019-11-28
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IGF-I for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2019-11-28
Question
Should the plate be washed after sample incubation and before antibody incubation steps for the Rat IGF-1 ELISA Kit PicoKine?
Verified customer
Asked: 2019-11-04
Answer
There isn't any wash step after sample incubation and before antibody incubation. Our lab technicains found there wasn't any significant difference in result valus between washing and without washing the plate after sample incubation and before antibody incubation.
Boster Scientific Support
Answered: 2019-11-05
Question
Q: if the enzyme conjugated IGF-I antibodies are mixed with the substrate, will that convert the substrate into the enzymatic reaction product? Or the enzyme function is only activated when the antibody is attached with the IGF-I antigen?
Z. Patel
Verified customer
Asked: 2019-09-25
Answer
A: The enzyme is always active. Avoid contaminating the substrate with enzyme prior to the incubation otherwise it compromises the assay with false positive signal.
Boster Scientific Support
Answered: 2019-09-25
Question
Can EK0377 measure total IGF-1 or free IGF-1?
Verified customer
Asked: 2019-06-13
Answer
The Rat IGF-1 ELISA Kit PicoKine™ (EK0377) detects free IGF-1. Usually, if the ELISA kit is not specific, it would detect total protein.
Boster Scientific Support
Answered: 2019-06-14
Question
Q: how many samples can be assayed in a Picokine® ELISA Kit?
K. Wu
Verified customer
Asked: 2019-03-22
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may vary slightly by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2019-03-22
Question
Q: is it okay to use citrate plasma as samples in Rat IGF-I Picokine® ELISA Kit (Catalog # EK0377)?
Verified Customer
Verified customer
Asked: 2019-03-06
Answer
A: Chelating agents such as EDTA, Heparin and Citrate can sequester metal ions from the functional domain of IGF-I causing disruption of its protein structure. IGF-I may be denatured as a result and may compromise the assay's measurements. The chilating sites could also be too close to the epitopes required for detection and block the antigen antibody reaction. We have tested the IGF-I ELISA, treating samples with various anticoagulants and decided that heparin or EDTA can be used for treatment of blood/plasma samples. Do not use other anticoagulents when collecting samples.
Boster Scientific Support
Answered: 2019-03-06
Question
Q: how to analyze ELISA data? I measured IGF-I level in plasma.
Verified Customer
Verified customer
Asked: 2018-10-16
Answer
A: please read this article on ELISA data analysis. bosterbio.com/ELISA-data-analysis-instructions. Boster also provides a free tool that you can use to analyze ELISA data. bosterbio.com/biology-research-tools/ELISA-data-analysis-online
Boster Scientific Support
Answered: 2018-10-16
Question
Q: how much samples can be assayed in a Picokine® ELISA Kit?
Verified Customer
Verified customer
Asked: 2018-10-12
Answer
A: The Picokine® ELISA Kits will generally run a 7-point standard curve, non-specific binding wells, and 39 samples in duplicate. this may might differ by kit so please refer to each datasheet for details.
Boster Scientific Support
Answered: 2018-10-12
Question
Q: What is the optimal O.D. value for IGF-I ELISA kit? I performed your IGF-I ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. is it safe to say these samples contain IGF-I even though the O.D. values are not very high?
P. Zhang
Verified customer
Asked: 2018-02-01
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. an important assessment should be is whether your sample O.D. values are statistically significantly higher than your blank values. considering your assay, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in relation to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by changeing cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IGF-I ELISA kit to have sensitivity of 5pg/ml, that means the minimum amount of IGF-I that can be declared/interpreted as positive by the above standard is 5pg/ml.
Boster Scientific Support
Answered: 2018-02-01
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Verified Customer
Verified customer
Asked: 2016-06-26
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is required for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2016-06-26
Question
Q: how to thaw whole blood sample for IGF-I ELISA after freezing?
W. Varma
Verified customer
Asked: 2014-06-28
Answer
A: we do not recommend freezing and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IGF-I for a later time, you should let the blood clot in glass tubes and collect the serum to freeze for later use.
Boster Scientific Support
Answered: 2014-06-28
Question
What is your recommended protocol on preparing tissues (such as bone) for ELISA analysis? Keyword: sample preparation, treatment procedures
Verified Customer
Verified customer
Asked: 2013-11-25
Answer
The protocol for bone tissue is as follows - Bone Tissue: Extract demineralized bone samples in 4 M Guanidine-HCl and protease inhibitors. Dissolve the final sample in 2 M Guanidine-HCl. However, we did not test tissue samples with the Elisa kits so a pilot experiment is recommended.
Boster Scientific Support
Answered: 2013-11-25
