Rat IL-10/Interleukin-10 ELISA Kit PicoKine®

IL-10 ELISA kit for Rat

Rat IL-10/Interleukin-10 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il10 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 4pg/ml. Cited in 10 publication(s).

Product Info Summary

SKU: EK0418
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (EDTA).

Product Name

Rat IL-10/Interleukin-10 ELISA Kit PicoKine®

View all IL-10 ELISA kits

SKU/Catalog Number

EK0418

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat IL-10/Interleukin-10 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il10 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 4pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)

Cite This Product

Rat IL-10/Interleukin-10 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0418)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S19-N178

Sensitivity

<4 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross-reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0418 is reactive to Il10 in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (EDTA).

Application Guarantee

EK0418 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Background of IL-10

Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.

Kit Components

Catalog Number Description Quantity
EK0418-CAP Anti-Rat Il10 Pre-coated 96-well strip microplate 1
EK0418-ST Rat Il10 Standard 2 vials, 10 ng/tube
EK0418-DA Rat Il10 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0120.0900.1700.2860.5431.0441.6492.076

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-10, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of IL-10 in samples: 25476076 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat IL-10/Interleukin-10 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5521383951222910
Standard deviation4.349.5836.074.7412.2139.13
CV (%)7.9%4.5%4.3%9.3%5.5%4.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-10 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 155585749543.496.4%
Sample 22132192322252227.043.1%
Sample 383990192582687241.394.7%
*number of samples for each test n=16.

Gene/Protein Information For Il10 (Source: Uniprot.Org, NCBI)

Uniprot ID

P29456

Gene ID

25325

Gene Name

Il10

Full Name

Interleukin-10

Weight

20.426kDa

Superfamily

IL-10 family

Alternative Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF Il10|IL10X, If2a|interleukin 10|interleukin-10|CSIF|IL-10|cytokine synthesis inhibitory factor|interferon 2a

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il10, check out the Il10 Infographic

Il10 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0418 has been cited in 10 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Cyanidin‑3‑O‑β‑glucoside protects against pulmonary artery hypertension induced by monocrotaline via the TGF‑β1/p38 MAPK/CREB signaling pathway

Role of CCR7 on dendritic cell‑mediated immune tolerance in the airways of allergy‑induced asthmatic rats

Allicin attenuates lipopolysaccharide‑induced acute lung injury in neonatal rats via the PI3K/Akt pathway

miR-130a alleviates neuronal apoptosis and changes in expression of Bcl-2/Bax and caspase-3 in cerebral infarction rats through PTEN/PI3K/Akt signaling pathway

A Study Based on Metabolomics, Network Pharmacology, and Experimental Verification to Explore the Mechanism of Qinbaiqingfei Concentrated Pills in the treatment of Mycoplasma Pneumonia

Bufei Yishen Formula Restores Th17/Treg Balance and Attenuates Chronic Obstructive Pulmonary Disease via Activation of the Adenosine 2a Receptor

The Potassium SK Channel Activator NS309 Protects Against Experimental Traumatic Brain Injury Through Anti-Inflammatory and Immunomodulatory Mechanisms

Saikosaponin A Inhibits Breast Cancer by Regulating Th1/Th2 Balance

Hyperbaric Oxygen Pretreatment Improves Cognition and Reduces Hippocampal Damage Via p38 Mitogen-Activated Protein Kinase in a Rat Model.

Effect of dexmedetomidine on kidney injury in sepsis rats through TLR4/MyD88/NF-κB/iNOS signaling pathway.

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11 Customer Q&As for Rat IL-10/Interleukin-10 ELISA Kit PicoKine®

Question

Q: how to thaw whole blood sample for IL-10 ELISA after freezing?

R. Kapoor

Verified customer

Asked: 2020-09-17

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-10 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-09-17

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-07-28

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-07-28

Question

What is the recommended dilution for rat serum samples using EK0418?

Verified customer

Asked: 2019-11-05

Answer

The recommended dilution for rat serum samples using the Rat IL-10 ELISA Kit PicoKine™ (EK0418) is 1:1.

Boster Scientific Support

Answered: 2019-11-05

Question

Is there a standardized total protein amount of samples that should be pipetted per well, so that it lies within the assay range of EK0418? Is protein quantification needed?

Verified customer

Asked: 2019-11-05

Answer

For the Rat IL-10 ELISA Kit PicoKine™ (EK0418), add 100ul of diluted sample into each well. It is unnecessary to quantify the amount of total protein.

Boster Scientific Support

Answered: 2019-11-05

Question

Is it possible to use plasma with heparin in the analyses of the EK0418?

Verified customer

Asked: 2019-10-24

Answer

The Rat IL-10 ELISA Kit PicoKine™ (EK0418) has not been tested with plasma samples with heparin. We cannot guarantee that it would work. It is suggested to run pilot tests to see if the kits would work on plasma samples with heparin if you would like to try.

Boster Scientific Support

Answered: 2019-10-24

Question

Could you tell me the size of the detected band of Rat IL-10 ELISA Kit PicoKine EK0418 in Western blotting? Thanks

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

The product Rat IL-10 ELISA Kit PicoKine EK0418 has MW of approximately 20kDa.

Boster Scientific Support

Answered: 2019-09-30

Question

I have further questions about kits: EK0418 , EK0514 and EK0393. About Sample Dilution: -Can I use the Bradford method to determine protein concentration? It says that final protein concentration should be calculated so that it lies in the middle of linear dynamic range of the assay. So, for example: If I consider (for IL1beta) 31.2pg/ml-2000pg/ml as the Assay Range, the middle of the assay range would be around 1000pg/ml. But this range refers to IL1beta, not total proteins. Measuring plasma total proteins would result in much higher protein levels when compared to IL1beta levels. Could you please explain me how should I proceed with these dilutions/protein quantification in more detail? Is there a standardized total protein amount of samples that should be pipetted per well, so that it lies within the assay range? I only found this general tip: "Serum, plasma, cell and tissue extracts are typically diluted by 50% with binding buffer". (ELISA sample preparation guide).

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

Please suggest the customer use the following receommeded dilution ratio for rat serum samples. EK0418 1:1 EK0514 1:100 EK0393 1:1 It is useless and unnecessary to quantify the amount of total protein. Add 100ul of diluted sample into each well.

Boster Scientific Support

Answered: 2019-06-28

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-05-24

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Z. Chen

Verified customer

Asked: 2018-09-10

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-09-10

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of IL-10 in Rat plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-04-13

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-10 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-04-13

Question

Q: What is the optimal O.D. value for IL-10 ELISA kit? I performed your IL-10 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-10 even though the O.D. values are not very high?

M. Parker

Verified customer

Asked: 2014-09-05

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-10 ELISA kit to have sensitivity of 4pg/ml, that means the minimum amount of IL-10 that can be declared/interpreted as positive by the above standard is 4pg/ml.

Boster Scientific Support

Answered: 2014-09-05

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