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Product Info Summary
| SKU: | EK0418 |
|---|---|
| Size: | 96 wells/kit, with removable strips. |
| Reactive Species: | Rat |
| Application: | ELISA |
| Sample Types: | cell culture supernatants, serum and plasma (EDTA). |
Product info
Product Name
Rat IL-10/Interleukin-10 ELISA Kit PicoKine®
SKU/Catalog Number
EK0418
Size
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Description
Rat IL-10/Interleukin-10 ELISA Kit PicoKine® (96 Tests). Quantitate Rat Il10 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 4pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers precise quantification, high sensitivity, and excellent reproducibility. Only our most reliable and effective kits qualify as Picokine, guaranteeing top-tier results for your assays.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Ships with gel ice, can store for up to 3 days in room temperature. Freeze upon receiving.)
Cite This Product
Rat IL-10/Interleukin-10 ELISA Kit PicoKine® (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0418)
Clonality of Antibodies
See Datasheet for details
Standard Protein
Expression system for standard: E.coli; Immunogen sequence: S19-N178
Capture/Detection Antibodies
The capture antibody is monoclonal antibody from mouse and the detection antibody is polyclonal antibody from goat.
Sensitivity
<4 pg/ml
Assay Range
31.2 pg/ml - 2,000 pg/ml
Standard Dilution Instructions
See datasheet of EK0418 for more details
Cross-reactivity
There is no detectable cross-reactivity with other relevant proteins.
Reactive Species
EK0418 is reactive to Il10 in Rat samples
Validated Sample Types
cell culture supernatants, serum and plasma (EDTA).
Application Guarantee
EK0418 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
Background of Il10
Interleukin-10, also called cytokine synthesis inhibitory factor, is implicated in tumorigenesis, and it has been shown that polymorphisms in its gene promoter correlate with differential amounts of production. IL-10 is an important cytokine with anti-inflammatory, anti-immune, and antifibrotic functions. It is also an important regulatory cytokine whose involvement extends into diverse areas of the human immune system. IL-10 is a recently described natural endogenous immunosuppressive cytokine that has been identified in human, murine, and other organisms. IL-10 significantly affects chemokine biology, because human IL-10 inhibits chemokine production and is a specific chemotactic factor for CD8+ T cells. It suppresses the ability of CD4+ T cells, but not CD8+ T cells, to migrate in response to IL-8. Interleukin-10 gene polymorphisms and interleukin-10 production capability may contribute to the development of skin squamous cell carcinomas after renal transplantation. The interleukin-10 locus contributes to the heritability of psoriasis susceptibility. With regard to sudden infant death, IL-10 is of special interest. This is an immunoregulatory cytokine that plays an important role in the development of infectious disease. The mIL-10 gene is mapped to mouse chromosome 1 and the hIL-10 gene is also mapped to human chromosome 1.
Kit Components
| Catalog Number | Description | Quantity |
|---|---|---|
| EK0418-CAP | Anti-Rat Il10 Pre-coated 96-well strip microplate | 1 |
| EK0418-ST | Rat Il10 Standard | 2 vials, 10 ng/tube |
| EK0418-DA | Rat Il10 Biotinylated antibody (100x) | 100ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex (100x) | 100ul |
| AR1106-1 | Sample Diluent | 30ml |
| AR1106-2 | Antibody Diluent | 12ml |
| AR1106-3 | Avidin-Biotin-Peroxidase Diluent | 12ml |
| AR1104 | Color Developing Reagent (TMB) | 10ml |
| AR1105 | Stop Solution | 10ml |
| AR1106-5 | Wash Buffer (25x) | 20ml |
| PLA-SEA | Adhesive plate sealers | 4 |
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Incubator.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
Data Examples, Quality Control Data & Sample Dilution
Validation Standard Curve O.D. At 450nm
| Concentration (pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.033 | 0.096 | 0.148 | 0.238 | 0.409 | 0.718 | 1.239 | 1.989 |
Data Example Images
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Rat IL-10 PicoKine ELISA Kit standard curve
Recommended Sample Dilution Ratios
According to our internal validation assays using this ELISA kit, to detect Il10, Dilution ratio of 1:1, concentration in serum and plasma is less than the lowest standard, 31.2 pg/ml..
Some articles we found to cite concentrations of Il10 in samples: 25476076 (Pubmed IDs).
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat IL-10/Interleukin-10 ELISA Kit PicoKine® within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision | |||||
|---|---|---|---|---|---|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean (pg/ml) | 55 | 213 | 839 | 51 | 222 | 910 |
| Standard deviation | 4.34 | 9.58 | 36.07 | 4.74 | 12.21 | 39.13 |
| CV (%) | 7.9% | 4.5% | 4.3% | 9.3% | 5.5% | 4.3% |
Specific Publications For Rat IL-10/Interleukin-10 ELISA Kit PicoKine® (EK0418)
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Customer Reviews
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Customer Q&As
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11 Customer Q&As for Rat IL-10/Interleukin-10 ELISA Kit PicoKine®
Question
Q: how to thaw whole blood sample for IL-10 ELISA after freezing?
R. Kapoor
Verified customer
Asked: 2020-09-17
Answer
A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-10 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.
Boster Scientific Support
Answered: 2020-09-17
Question
Q: is there any online tool I can use to streamline the data analysis for my ELISA results?
Verified Customer
Verified customer
Asked: 2020-07-28
Answer
A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/elisa-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/elisa-data-analysis-instructions
Boster Scientific Support
Answered: 2020-07-28
Question
What is the recommended dilution for rat serum samples using EK0418?
Verified customer
Asked: 2019-11-05
Answer
The recommended dilution for rat serum samples using the Rat IL-10 ELISA Kit PicoKine™ (EK0418) is 1:1.
Boster Scientific Support
Answered: 2019-11-05
Question
Is there a standardized total protein amount of samples that should be pipetted per well, so that it lies within the assay range of EK0418, EK0514 and EK0393? Is protein quantification needed?
Verified customer
Asked: 2019-11-05
Answer
For the Rat IL-10 ELISA Kit PicoKine™ (EK0418), Rat TGF Beta 1 ELISA Kit PicoKine™ (EK0514) and Rat IL-1 Beta ELISA Kit PicoKine™ (EK0393), add 100ul of diluted sample into each well. It is unnecessary to quantify the amount of total protein.
Boster Scientific Support
Answered: 2019-11-05
Question
Is it possible to use plasma with heparin in the analyses of the EK0418?
Verified customer
Asked: 2019-10-24
Answer
The Rat IL-10 ELISA Kit PicoKine™ (EK0418) has not been tested with plasma samples with heparin. We cannot guarantee that it would work. It is suggested to run pilot tests to see if the kits would work on plasma samples with heparin if you would like to try.
Boster Scientific Support
Answered: 2019-10-24
Question
Could you tell me the size of the detected band of Rat IL-10 ELISA Kit PicoKine EK0418 in Western blotting? Thanks
Verified Customer
Verified customer
Asked: 2019-09-30
Answer
The product Rat IL-10 ELISA Kit PicoKine EK0418 has MW of approximately 20kDa.
Boster Scientific Support
Answered: 2019-09-30
Question
I have further questions about kits: EK0418 , EK0514 and EK0393. About Sample Dilution: -Can I use the Bradford method to determine protein concentration? It says that final protein concentration should be calculated so that it lies in the middle of linear dynamic range of the assay. So, for example: If I consider (for IL1beta) 31.2pg/ml-2000pg/ml as the Assay Range, the middle of the assay range would be around 1000pg/ml. But this range refers to IL1beta, not total proteins. Measuring plasma total proteins would result in much higher protein levels when compared to IL1beta levels. Could you please explain me how should I proceed with these dilutions/protein quantification in more detail? Is there a standardized total protein amount of samples that should be pipetted per well, so that it lies within the assay range? I only found this general tip: "Serum, plasma, cell and tissue extracts are typically diluted by 50% with binding buffer". (ELISA sample preparation guide).
Verified Customer
Verified customer
Asked: 2019-06-28
Answer
Please suggest the customer use the following receommeded dilution ratio for rat serum samples. EK0418 1:1 EK0514 1:100 EK0393 1:1 It is useless and unnecessary to quantify the amount of total protein. Add 100ul of diluted sample into each well.
Boster Scientific Support
Answered: 2019-06-28
Question
Q: how are cell lysates prepared for use in Picokine® ELISA kits?
Verified Customer
Verified customer
Asked: 2019-05-24
Answer
A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.
Boster Scientific Support
Answered: 2019-05-24
Question
Q: Are Boster Bio recombinant proteins and antibodies sterile?
Z. Chen
Verified customer
Asked: 2018-09-10
Answer
A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.
Boster Scientific Support
Answered: 2018-09-10
Question
Q: we need your suggestion regarding the dilution ratio of serum samples for detection of IL-10 in Rat plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.
Verified Customer
Verified customer
Asked: 2018-04-13
Answer
A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-10 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/elisa-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.
Boster Scientific Support
Answered: 2018-04-13
Question
Q: What is the optimal O.D. value for IL-10 ELISA kit? I performed your IL-10 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-10 even though the O.D. values are not very high?
M. Parker
Verified customer
Asked: 2014-09-05
Answer
A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-10 ELISA kit to have sensitivity of 4pg/ml, that means the minimum amount of IL-10 that can be declared/interpreted as positive by the above standard is 4pg/ml.
Boster Scientific Support
Answered: 2014-09-05
