Rat IL-10/Interleukin-10 ELISA Kit PicoKine™

Rat IL-10/Interleukin-10 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il10 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 4pg/ml. Cited in 77 publication(s).

Product Info Summary

SKU: EK0418
Size: 96 wells/kit, with removable strips.
Reactive Species: Rat
Application: ELISA
Sample Types: cell culture supernatants, serum and plasma (EDTA).

Product Name

Rat IL-10/Interleukin-10 ELISA Kit PicoKine™

See all IL-10 products

SKU/Catalog Number

EK0418

Size

96 wells/kit, with removable strips.

*Question: How many samples can I assay/run in this kit?

Description

Rat IL-10/Interleukin-10 ELISA Kit PicoKine™ (96 Tests). Quantitate Rat Il10 in cell culture supernatants, serum and plasma (EDTA). Sensitivity: 4pg/ml.

Storage & Handling

Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Cite This Product

Rat IL-10/Interleukin-10 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0418)

Clonality of Antibodies

See Datasheet for details

Immunogen

Expression system for standard: E.coli; Immunogen sequence: S19-N178

Sensitivity

<4 pg/ml

Assay Range

31.2 pg/ml - 2,000 pg/ml

Cross Reactivity

There is no detectable cross-reactivity with other relevant proteins.

Reactive Species

EK0418 is reactive to Il10 in Rat samples

Validated Sample Types

cell culture supernatants, serum and plasma (EDTA).

Application Guarantee

EK0418 is guaranteed for ELISA in Rat by Boster Guarantee

See how Boster Bio validate our ELISA kits: ELISA Validation Information

Kit Components

Catalog Number Description Quantity
EK0418-CAP Anti-Rat Il10 Pre-coated 96-well strip microplate 1
EK0418-ST Rat Il10 Standard 2 vials, 10 ng/tube
EK0418-DA Rat Il10 Biotinylated antibody (100x) 100ul
AR1103 Avidin-Biotin-Peroxidase Complex (100x) 100ul
AR1106-1 Sample Diluent 30ml
AR1106-2 Antibody Diluent 12ml
AR1106-3 Avidin-Biotin-Peroxidase Diluent 12ml
AR1104 Color Developing Reagent (TMB) 10ml
AR1105 Stop Solution 10ml
AR1106-5 Wash Buffer (25x) 20ml
PLA-SEA Adhesive plate sealers 4

*The kit components are not available for individual purchase.

Materials Required But Not Included In Kit

  • Microplate Reader capable of reading absorbance at 450nm.
  • Automated plate washer (optional).
  • Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
  • Multichannel pipettes are recommended for large amount of samples.
  • Deionized or distilled water.
  • 500ml graduated cylinders.
  • Test tubes for dilution.

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Validation Standard Curve O.D. At 450nm

Concentration (pg/ml)031.262.512525050010002000
O.D.0.0120.0900.1700.2860.5431.0441.6492.276

Data Example Images

Recommended Sample Dilution Ratios

According to our internal validation assays using this ELISA kit, to detect IL-10, Dilution ratio of 1:1, concentration in serum is target in serum is below detection limit..

Some articles we found to cite concentrations of IL-10 in samples: 25476076 (Pubmed IDs).

Intra Assay Consistency & Inter Assay Consistency

We measured random samples of Rat IL-10/Interleukin-10 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.

Intra-Assay PrecisionInter-Assay Precision
Sample123123
n161616242424
Mean (pg/ml)5521383951222910
Standard deviation4.349.5836.074.7412.2139.13
CV (%)7.9%4.5%4.3%9.3%5.5%4.3%

Reproducibility

We ensure reproducibility by testing three samples with differing concentrations of IL-10 in ELISA kits from four different production batches/lots.

LotsLot 1 (pg/ml)Lot 2 (pg/ml)Lot 3 (pg/ml)Lot 4 (pg/ml)Mean (pg/ml)Standard DeviationCV (%)
Sample 155585749543.496.4%
Sample 22132192322252227.043.1%
Sample 383990192582687241.394.7%
*number of samples for each test n=16.

Gene/Protein Information For Il10 (Source: Uniprot.Org, NCBI)

Uniprot ID

P29456

Gene ID

25325

Gene Name

Il10

Full Name

Interleukin-10

Weight

20.426kDa

Superfamily

IL-10 family

Alternative Names

CSIF; CSIFMGC126450; Cytokine synthesis inhibitory factor; GVHDS; IL10; IL-10; IL10A; IL-10MGC126451; interleukin 10; interleukin-10; TGIF Il10|IL10X, If2a|interleukin 10|interleukin-10|CSIF|IL-10|cytokine synthesis inhibitory factor|interferon 2a

*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".

For more info on Il10, check out the Il10 Infographic

Il10 infographic

We have 30,000+ of these available, one for each gene! check them out.

In this infographic you will see the following information for Il10: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]

EK0418 has been cited in 77 publications:

*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.

Xi H,Tao T,Zhang R,Xue X,Zhu Y,Liu J,Xin X,Zeng X.The 2-(2-benzofuranyl)-2-imidazoline provides neuroprotection against focal cerebral ischemia-reperfusion injury in diabetic rats: Influence of microglia and possible mechanisms of action.Brain Res Bull.2021 Jun 24;174:230-239.doi:10.1016/j.brainresbull.2021.06.016.Epub ahead of print.PMID:34175385.
Species: Rat

Yan Jiang,Li Zhao,Qing Chen,Lihong Zhou,"Exploring the Mechanism of Berberine Intervention in Ulcerative Colitis from the Perspective of Inflammation and Immunity Based on Systemic Pharmacology",Evidence-Based Complementary and Alternative Medicine,vol.2021,Article ID 9970240,11 pages,2021.https://doi.org/ 10.1155/2021/9970240
Species: Rat
EK0418 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Lipopolysaccharides,I.C.I.B.B.,Fesal,M.,Seif,F.,Abdella,A.,& Amin,H.M.Middle East Journal of Applied Sciences Volume:11| Issue: 01|Jan.-March| 2021.
Species: Rat
EK0418 usage in article: APP:ELISA, SAMPLE:LIVER TISSUE, DILUTION:NA

Effect of α-Tocopherol on Cytokines Production, Oxidative Stress, and Liver Injury Induced by Lipopolysaccharides in Male Albino Rats. Amin,H. M.
Species: Rat
EK0418 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Cheng Wang,Liang Xian,Shaorui Zheng et al.Cranial Venous Outflow Obstruction Promotes Neuroinflammation And Edema Via ADAM17/solTNF-α/NF-κB Pathway In A Rat Subdural Hematoma Model,11 May 2021,PREPRINT (Version 1) available at Research Square [https://doi.org/10.21203/rs.3.rs-487715/v1]
Species: Rat
EK0418 usage in article: APP:ELISA, SAMPLE:BRAIN TISSUE, DILUTION:NA

Ouyang, S.,Chen, W.,Gaofeng, Z.,Changcheng, L.,Guoping, T.,Minyan, Z. ... Luo, J. (2021). Cyanidin‑3‑O‑β‑glucoside protects against pulmonary artery hypertension induced by monocrotaline via the TGF‑β1/p38 MAPK/CREB signaling pathway. Molecular Medicine Reports,23, 338.https://doi.org/10.3892/mmr.2021.11977
Species: Human,Rat
EK0418 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Liu Yuan,Luo Pei,Zhang Ji-Zhong,Chen Xiao-Yi,Li Li-Min,Wu You-Jiao,Yin Qiao-Zhi,Huang Li.Identification of Active Compounds From Yi Nationality Herbal Formula Wosi Influencing COX-2 and VCAM-1 Signaling. Frontiers in Pharmacology,11.2020.1690.https://www.
Species: Rat
EK0418 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Jiang,Guoyong and Lei,AItong and Chen,Yi and Yu,Qiang and Xie,Jianhua and Yang,Ying and Yuan,Tongji and Su, Dan", "Protective effects of Ganoderma atrum polysaccharide against acrylamide-induced inflam mation and oxidative damage in rats","Food Funct.","2
Species: Rat
EK0418 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Wang Y,Zhang L,Li L,Hu H,Pan P,Zhang B,Ning W,Zhao M,Wang S.Electroacupuncture Improves Blood Pressure in SHRs by Regulating the Immune Balance between Th17 and Treg.Evid Based Complement Alternat Med.2020 Jul 7;2020:5375981.doi:10.1155/2020/5375981.PMID:
Species: Rat
EK0418 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Liu Y,Zhang X,Yan D,Wang Y,Wang N,Liu Y,Tan A,Chen X,Yan H.Chronic acrylamide exposure induced glia cell activation, NLRP3 infl-ammasome upregulation and cognitive impairment.Toxicol Appl Pharmacol.2020 Apr 15;393:114949.doi:10.1016/j.taap.2020.114949.Epu
Species: Rat
EK0418 usage in article: APP:ELISA, SAMPLE:SERUM, DILUTION:NA

Wang Q, Xu GX, Tai QH, Wang Y. Lipoxin A4 Reduces Ventilator-Induced Lung Injury in Rats with Large-Volume Mechanical Ventilation. Mediators Inflamm. 2020 Nov 22;2020:6705985. doi: 10.1155/2020/6705985. PMID: 33299377; PMCID: PMC7704204.
Species: Rat
EK0418 usage in article: APP:ELISA, SAMPLE:BALF, DILUTION:NA

Effects of Zinc Finger Protein A20 on Lipopolysaccharide (LPS)-Induced Pulmonary Inflammation/Anti-Inflammatory Mediators in an Acute Lung Injury/Acute %u2026

IL-10/STAT3 is reduced in childhood obesity with hypertriglyceridemia and is related to triglyceride level in diet-induced obese rats

Hyperbaric Oxygen Pretreatment Improves Cognition and Reduces Hippocampal Damage Via p38 Mitogen-Activated Protein Kinase in a Rat Model

Sequential Treatments with Tongsai and Bufei Yishen Granules Reduce Inflammation and Improve Pulmonary Function in Acute Exacerbation-Risk Window of Chronic Obstructive Pulmonary Disease in Rats

Antioxidant and Anti-Inflammatory Effects of Coenzyme Q10 on L-Arginine-Induced Acute Pancreatitis in Rat

Curcumin exerts anti-inflammatory and antioxidative properties in 1-methyl-4-phenylpyridinium ion (MPP(+))-stimulated mesencephalic astrocytes by interference with TLR4 and downstream signaling pathway

Extracts of Bauhinia championii (Benth) Benth attenuate the in?ammatory response in a rat model of collagen-induced arthritis

Wound healing treatment by high frequency ultrasound, microcurrent, and combined therapy modifies the immune response in rats

Preconditioning with VEGF Enhances Angiogenic and Neuroprotective Effects of Bone Marrow Mononuclear Cell Transplantation in a Rat Model of Chronic Cerebral Hypoperfusion

Epinephrine Enhances the Response of Macrophages under LPS Stimulation

Suppression of complete Freund's adjuvant-induced adjuvant arthritis by cobratoxin

Gao D, Jing S, Zhang Q, Wu G. Exp Ther Med. 2018 Jan;15(1):1029-1035. doi: 10.3892/etm.2017.5479. Epub 2017 Nov 10. Pterostilbene protects against acute renal ischemia reperfusion injury and inhibits oxidative stress, inducible nitric oxide syntha...

Chen C, Chen W, Li Y, Dong Y, Teng X, Nong Z, Pan X, Lv L, Gao Y, Wu G. Oncotarget. 2017 Dec 4;8(67):111522-111534. doi: 10.18632/oncotarget.22869. eCollection 2017 Dec 19. Hyperbaric oxygen protects against myocardial reperfusion injury via the i...

He X, Cui LH, Wang XH, Yan ZH, Li C, Gong SD, Zheng Y, Luo Z, Wang Y. Oncotarget. 2017 Dec 8;8(69):113957-113965. doi: 10.18632/oncotarget.23045. eCollection 2017 Dec 26. Modulation of inflammation by toll-like receptor 4/nuclear factor-kappa B in...

Sarandy MM, Miranda LL, Alto

Lin X, Ju YN, Gao W, Li DM, Guo CC. Biomed Res Int. 2018 Jan 29;2018:7507314. doi: 10.1155/2018/7507314. eCollection 2018. Desflurane Attenuates Ventilator-Induced Lung Injury in Rats with Acute Respiratory Distress Syndrome

Zhu R, Guo W, Fang H, Cao S, Yan B, Chen S, Zhang K, Zhang S. Mol Med Rep. 2018 May;17(5):6357-6362. doi: 10.3892/mmr.2018.8646. Epub 2018 Feb 27. Kupffer cell depletion by gadolinium chloride aggravates liver injury after brain death in rats

Chen X, Chen C, Fan S, Wu S, Yang F, Fang Z, Fu H, Li Y. J Neuroinflammation. 2018 Apr 20;15(1):116. doi: 10.1186/s12974-018-1151-3. Omega-3 polyunsaturated fatty acid attenuates the inflammatory response by modulating microglia polarization throu...

Yang H, Wu S, Feng R, Huang J, Liu L, Liu F, Chen Y. Stem Cell Res Ther. 2017 Nov 21;8(1):267. doi: 10.1186/s13287-017-0718-8. Vitamin C plus hydrogel facilitates bone marrow stromal cell-mediated endometrium regeneration in rats

Zhang S, Li J, Li Y, Liu Y, Guo H, Xu X. Front Physiol. 2017 Apr 27;8:249. doi: 10.3389/fphys.2017.00249. eCollection 2017. Nitric Oxide Synthase Activity Correlates with OGG1 in Ozone-Induced Lung Injury Animal Models

Gao, W., Meng, Q.M., Cui, X.G. (2017). Budesonide instillation immediately after reperfusion ameliorates ischemia/reperfusion-induced injury in the transplanted lung of rat. Experimental Lung Research, 43(9-10), 439-446. doi: 10.1080/01902148.2017...

Ye J, Caihong S, Yayan H, Zhang X, Meitian X. J Sci Food Agric. 2017 Mar 23. doi: 10.1002/jsfa.8322. Anti-fatigue activity of sea cucumber peptides prepared from Stichopus japonicas in an endurance swimming rat model.

Baisong Zhao, Yongying Pan, Zixin Wang, Haiping Xu and Xingrong Song. Yonsei Med J. 2017 Jan;58(1):131-138. Hyperbaric Oxygen Pretreatment Improves Cognition and Reduces Hippocampal Damage Via p38 Mitogen-Activated Protein Kinase in a Rat Model

Wang T, Zhou Yt, Chen Xn, Zhu Ax. Braz J Med Biol Res. 2014 Sep;47(9):738-45. Epub 2014 Jul 25. Putative Role Of Ischemic Postconditioning In A Rat Model Of Limb Ischemia And Reperfusion: Involvement Of Hypoxia-Inducible Factor-1?? Expression.

Zhang Y, Leng Yf, Xue X, Zhang Y, Wang T, Kang Yq. World J Gastroenterol. 2011 Jan 14;17(2):254-9. Doi: 10.3748/Wjg.V17.I2.254. Effects Of Penehyclidine Hydrochloride In Small Intestinal Damage Caused By Limb Ischemia-Reperfusion.

Luo H,, Wang X,, Wang J,, Chen C,, Wang N,, Xu Z,, Chen S,, Zeng C,. Cardiovasc Diabetol. 2015 Jun 10;14:76. Doi: 10.1186/S12933-015-0239-7. Chronic Nf-??b Blockade Improves Renal Angiotensin Ii Type 1 Receptor Functions And Reduces Blood Pressure...

Wang T, Zhou Yt, Chen Xn, Zhu Ax, Wu Bh. World J Gastroenterol. 2014 Jul 28;20(28):9519-27. Doi: 10.3748/Wjg.V20.I28.9519. Remote Ischemic Postconditioning Protects Against Gastric Mucosal Lesions In Rats.

Zhang X, Liu F, Liu H, Cheng H, Wang W, Wen Q, Wang Y. Inflamm Res. 2011 Jun;60(6):569-75. Doi: 10.1007/S00011-010-0305-2. Epub 2011 Jan 19. Urinary Trypsin Inhibitor Attenuates Lipopolysaccharide-Induced Acute Lung Injury By Blocking The Activati...

Chen T, Liu W, Chao X, Zhang L, Qu Y, Huo J, Fei Z. Brain Res Bull. 2011 Feb 1;84(2):163-8. Doi: 10.1016/J.Brainresbull.2010.11.015. Epub 2010 Dec 4. Salvianolic Acid B Attenuates Brain Damage And Inflammation After Traumatic Brain Injury In Mice.

Li Xj, Liu Dp, Chen Hl, Pan Xh, Kong Qy, Pang Qf. Int Immunopharmacol. 2012 Feb;12(2):460-4. Doi: 10.1016/J.Intimp.2012.01.001. Epub 2012 Jan 15. Lactoferrin Protects Against Lipopolysaccharide-Induced Acute Lung Injury In Mice.

Xia J, Zhou Xl, Zhao Y, Zhu Yq, Jiang S, Ni Sz. Inflammation. 2013 Dec;36(6):1431-9. Doi: 10.1007/S10753-013-9683-2. Roles Of Lipoxin A4 In Preventing Paracetamol-Induced Acute Hepatic Injury In A Rabbit Model.

Tang Y, Liao Y, Kawaguchi-Sakita N, Raut V, Fakhrejahani E, Qian N, Toi M. J Hepatobiliary Pancreat Sci. 2011 Jul;18(4):551-8. Doi: 10.1007/S00534-010-0368-Z. Sinisan, A Traditional Chinese Medicine, Attenuates Experimental Chronic Pancreatitis In...

Chen W, Qi J, Feng F, Wang Md, Bao G, Wang T, Xiang M, Xie Wf. Neurochem Int. 2014 Mar;68:28-37. Doi: 10.1016/J.Neuint.2014.01.015. Epub 2014 Feb 12. Neuroprotective Effect Of Allicin Against Traumatic Brain Injury Via Akt/Endothelial Nitric Oxide...

Ye Xh, Wu Y, Guo Pp, Wang J, Yuan Sy, Shang Y, Yao Sl. Brain Res. 2010 Apr 6;1323:174-83. Doi: 10.1016/J.Brainres.2010.01.079. Epub 2010 Feb 4. Lipoxin A4 Analogue Protects Brain And Reduces Inflammation In A Rat Model Of Focal Cerebral Ischemia R...

Jin Y, Wan Y, Chen G, Chen L, Zhang Mq, Deng L, Zhang Jc, Xiong Xz, Xin Jb. Plos One. 2014 Oct 16;9(10):E111044. Doi: 10.1371/Journal.Pone.0111044. Ecollection 2014. Treg/Il-17 Ratio And Treg Differentiation In Patients With Copd.

Sereshki N, Gharagozloo M, Ostadi V, Ghahiri A, Roghaei Ma, Mehrabian F, Andalib Aa, Hassanzadeh A, Hosseini H, Rezaei A. Int J Fertil Steril. 2014 Apr;8(1):59-66. Epub 2014 Mar 9. Variations In T-Helper 17 And Regulatory T Cells During The Menstr...

Hualin C, Wenli X, Dapeng L, Xijing L, Xiuhua P, Qingfeng P. Inflammation. 2012 Jun;35(3):1087-93. Doi: 10.1007/S10753-011-9415-4. The Anti-Inflammatory Mechanism Of Heme Oxygenase-1 Induced By Hemin In Primary Rat Alveolar Macrophages.

Zhou X, Cao Y, Ao G, Hu L, Liu H, Wu J, Wang X, Jin M, Zheng S, Zhen X, Alkayed Nj, Jia J, Cheng J. Antioxid Redox Signal. 2014 Oct 20;21(12):1741-58. Doi: 10.1089/Ars.2013.5587. Epub 2014 Mar 13. Camkk??-Dependent Activation Of Amp-Activated Prot...

Sun Hx, Chen Lq, Zhang J, Chen Fy. J Ethnopharmacol. 2014 May 14;153(3):831-9. Doi: 10.1016/J.Jep.2014.03.052. Epub 2014 Mar 29. Anti-Tumor And Immunomodulatory Activity Of Peptide Fraction From The Larvae Of Musca Domestica.

Ruan Z, Liu S, Zhou Y, Mi S, Liu G, Wu X, Yao K, Assaad H, Deng Z, Hou Y, Wu G, Yin Y. Plos One. 2014 Jun 2;9(6):E97815. Doi: 10.1371/Journal.Pone.0097815. Ecollection 2014. Chlorogenic Acid Decreases Intestinal Permeability And Increases Expressi...

Meng A, Wang B, Zhang X, Qi N, Liu D, Wu J. Inflammation. 2015;38(3):1260-6. Doi: 10.1007/S10753-014-0093-X. Additive Suppression Of Lps-Induced Il-10 And Tnf-?? By Pre-Treatment Of Dexamethasone And Sb203580 In A Murine Alveolar Macrophage Cell L...

Chen W, Guo Y, Yang W, Zheng P, Zeng J, Tong W. Exp Brain Res. 2015 Oct;233(10):2823-31. Doi: 10.1007/S00221-015-4352-3. Epub 2015 Jun 13. Protective Effect Of Ginsenoside Rb1 On Integrity Of Blood-Brain Barrier Following Cerebral Ischemia.

Li J, Yang L, Yao Q, Li Y, Tian Y, Li S, Jiang S, Wang Y, Li X, Guo Z. Evid Based Complement Alternat Med. 2014;2014:381976. Doi: 10.1155/2014/381976. Epub 2014 May 14. Effects And Mechanism Of Bufei Yishen Formula In A Rat Chronic Obstructive Pul...

Li Y, Li Sy, Li Js, Deng L, Tian Yg, Jiang Sl, Wang Y, Wang Yy. Biol Pharm Bull. 2012;35(10):1752-60. Epub 2012 Aug 3. A Rat Model For Stable Chronic Obstructive Pulmonary Disease Induced By Cigarette Smoke Inhalation And Repetitive Bacterial Infe...

Niu Z, Ma J, Chu H, Zhao Y, Feng W, Cheng Y. Neurosci Lett. 2012 Nov 7;529(2):112-7. Doi: 10.1016/J.Neulet.2012.09.034. Epub 2012 Sep 27. Melanocortin 4 Receptor Antagonists Attenuates Morphine Antinociceptive Tolerance, Astroglial Activation And ...

Liu W, Dong Xs, Sun Yq, Liu Z. Int J Clin Exp Med. 2014 Apr 15;7(4):919-26. Ecollection 2014. A Novel Fluid Resuscitation Protocol: Provide More Protection On Acute Kidney Injury During Septic Shock In Rats.

Zhang Gb, Tian Lq, Li Ym, Liao Yf, Li J, Bing Fh. Phytomedicine. 2013 Aug 15;20(11):964-8. Doi: 10.1016/J.Phymed.2013.04.009. Epub 2013 Jun 6. Protective Effect Of Homonojirimycin From Commelina Communis (Dayflower) On Influenza Virus Infection In...

Yin Y, Qin T, Yu Q, Yang Q. Amino Acids. 2014 Jul;46(7):1763-74. Doi: 10.1007/S00726-014-1735-X. Epub 2014 Apr 8. Bursopentin (Bp5) From Chicken Bursa Of Fabricius Attenuates The Immune Function Of Dendritic Cells.

Tang D, Gao J, Wang S, Yuan Z, Ye N, Chong Y, Xu C, Jiang X, Li B, Yin W, Miao Y, Wang D, Jiang K. Tumour Biol. 2015 Jul;36(7):5617-26. Doi: 10.1007/S13277-015-3233-5. Epub 2015 Mar 1. Apoptosis And Anergy Of T Cell Induced By Pancreatic Stellate ...

Chen X, Zhao C, Li X, Wang T, Li Y, Cao C, Ding Y, Dong M, Finci L, Wang Jh, Li X, Liu L. Nat Chem Biol. 2015 Jan;11(1):19-25. Doi: 10.1038/Nchembio.1657. Epub 2014 Nov 10. Terazosin Activates Pgk1 And Hsp90 To Promote Stress Resistance.

Liu R, An L, Liu G, Li X, Tang W, Chen X. Antiviral Res. 2015 Aug;120:101-11. Doi: 10.1016/J.Antiviral.2015.05.008. Epub 2015 May 27. Mouse Lung Slices: An Ex Vivo Model For The Evaluation Of Antiviral And Anti-Inflammatory Agents Against Influenz...

Dai M, Wu L, He Z, Zhang S, Chen C, Xu X, Wang P, Gruzdev A, Zeldin Dc, Wang Dw. J Cell Physiol. 2015 Sep;230(9):2108-19. Doi: 10.1002/Jcp.24939. Epoxyeicosatrienoic Acids Regulate Macrophage Polarization And Prevent Lps-Induced Cardiac Dysfunction.

Zhang Y, Zhang S, Li H, Huang M, Xu W, Chu K, Chen L, Chen X. Mol Med Rep. 2015 Jul;12(1):988-94. Doi: 10.3892/Mmr.2015.3515. Epub 2015 Mar 19. Ameliorative Effects Of Gualou Guizhi Decoction On Inflammation In Focal Cerebral Ischemic-Reperfusion ...

Shi Jq, Chen J, Wang Br, Zhu Yw, Xu Y, Wang J, Xiao H, Shi Jp, Zhang Yd, Xu J. Peptides. 2011 Aug;32(8):1617-25. Doi: 10.1016/J.Peptides.2011.06.013. Epub 2011 Jun 21. Short Amyloid-Beta Immunogens Show Strong Immunogenicity And Avoid Stimulating ...

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Wang Sb, Deng Yq, Ren J, Xiao Bk, Chen Z, Tao Zz. Scand J Immunol. 2013 Dec;78(6):507-15. Doi: 10.1111/Sji.12118. Lactoferrin Administration Into The Nostril Alleviates Murine Allergic Rhinitis And Its Mechanisms.

Huang L, Cao J. Oxid Med Cell Longev. 2014;2014:248786. Doi: 10.1155/2014/248786. Epub 2014 Mar 5. The Protective Effects Of Shen-Fu Injection On Experimental Acute Pancreatitis In A Rat Model.

Ou J, Shi W, Xu Y, Tao Z. Ann Allergy Asthma Immunol. 2014 Dec;113(6):658-665.E1. Doi: 10.1016/J.Anai.2014.08.015. Epub 2014 Sep 17. Intranasal Immunization With Dna Vaccine Coexpressing Der P 1 And Ubiquitin In An Allergic Rhinitis Mouse Model.

Jin Q, Cheng J, Liu Y, Wu J, Wang X, Wei S, Zhou X, Qin Z, Jia J, Zhen X. Brain Behav Immun. 2014 Aug;40:131-42. Doi: 10.1016/J.Bbi.2014.03.003. Epub 2014 Mar 12. Improvement Of Functional Recovery By Chronic Metformin Treatment Is Associated With...

Ozkan Zs, Deveci D, Kumbak B, Simsek M, Ilhan F, Sekercioglu S, Sapmaz E. J Reprod Immunol. 2014 Jun;103:53-8. Doi: 10.1016/J.Jri.2013.11.002. Epub 2013 Dec 8. What Is The Impact Of Th1/Th2 Ratio, Socs3, Il17, And Il35 Levels In Unexplained Infert...

Presti I, D'Orazio G, Labra M, La Ferla B, Mezzasalma V, Bizzaro G, Giardina S, Michelotti A, Tursi F, Vassallo M, Di Gennaro P. Appl Microbiol Biotechnol. 2015 Jul;99(13):5613-26. Doi: 10.1007/S00253-015-6482-8. Epub 2015 Mar 7. Evaluation Of The...

Chen F, Ni Y, Ye Y, Sun H, Li X, Xu S. Int Immunopharmacol. 2010 Oct;10(10):1153-60. Doi: 10.1016/J.Intimp.2010.06.021. Epub 2010 Jul 4. Comparison Of Immunosuppressive Activity Of Stephanoside E And Its Aglycone From Stephanotis Mucronata In Vitro.

Yu Zm, Liu Zh, Chen J, Zeng Q. Chin J Integr Med. 2014 Dec;20(12):934-43. Doi: 10.1007/S11655-014-1863-X. Epub 2014 Nov 27. Anti-Inflammatory Effect Of Qingwen Baidu Decoction (????????????

Zhu Hc, Qiu T, Liu Xh, Dong Wc, Weng Xd, Hu Ch, Kuang Yl, Gao Rh, Dan C, Tao T. Cell Immunol. 2012;274(1-2):12-8. Doi: 10.1016/J.Cellimm.2012.02.012. Epub 2012 Mar 14. Tolerogenic Dendritic Cells Generated By Relb Silencing Using Shrna Prevent Acu...

Guida F, Luongo L, Aviello G, Palazzo E, De Chiaro M, Gatta L, Boccella S, Marabese I, Zjawiony Jk, Capasso R, Izzo Aa, De Novellis V, Maione S. Mol Pain. 2012 Aug 23;8:60. Doi: 10.1186/1744-8069-8-60. Salvinorin A Reduces Mechanical Allodynia And...

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11 Customer Q&As for Rat IL-10/Interleukin-10 ELISA Kit PicoKine™

Question

Q: how to thaw whole blood sample for IL-10 ELISA after freezing?

R. Kapoor

Verified customer

Asked: 2020-09-17

Answer

A: do not freeze and thaw whole blood. erythrocytes are fragile and, if frozen and thawed, will undergo hemolysis rendering the samples useless. To keep your blood samples to test IL-10 for a later time, you should let the blood clot in glass tubes and separate the serum to freeze for later analysis.

Boster Scientific Support

Answered: 2020-09-17

Question

Q: is there any online tool I can use to streamline the data analysis for my ELISA results?

Verified Customer

Verified customer

Asked: 2020-07-28

Answer

A: We have a web based ELISA curve fitting (4pl) and data analysis tool. Please give it a try: bosterbio.com/biology-research-tools/ELISA-data-analysis-online. You can also consult our article on ELISA data analysis: bosterbio.com/ELISA-data-analysis-instructions

Boster Scientific Support

Answered: 2020-07-28

Question

What is the recommended dilution for rat serum samples using EK0418?

Verified customer

Asked: 2019-11-05

Answer

The recommended dilution for rat serum samples using the Rat IL-10 ELISA Kit PicoKine™ (EK0418) is 1:1.

Boster Scientific Support

Answered: 2019-11-05

Question

Is there a standardized total protein amount of samples that should be pipetted per well, so that it lies within the assay range of EK0418? Is protein quantification needed?

Verified customer

Asked: 2019-11-05

Answer

For the Rat IL-10 ELISA Kit PicoKine™ (EK0418), add 100ul of diluted sample into each well. It is unnecessary to quantify the amount of total protein.

Boster Scientific Support

Answered: 2019-11-05

Question

Is it possible to use plasma with heparin in the analyses of the EK0418?

Verified customer

Asked: 2019-10-24

Answer

The Rat IL-10 ELISA Kit PicoKine™ (EK0418) has not been tested with plasma samples with heparin. We cannot guarantee that it would work. It is suggested to run pilot tests to see if the kits would work on plasma samples with heparin if you would like to try.

Boster Scientific Support

Answered: 2019-10-24

Question

Could you tell me the size of the detected band of Rat IL-10 ELISA Kit PicoKine EK0418 in Western blotting? Thanks

Verified Customer

Verified customer

Asked: 2019-09-30

Answer

The product Rat IL-10 ELISA Kit PicoKine EK0418 has MW of approximately 20kDa.

Boster Scientific Support

Answered: 2019-09-30

Question

I have further questions about kits: EK0418 , EK0514 and EK0393. About Sample Dilution: -Can I use the Bradford method to determine protein concentration? It says that final protein concentration should be calculated so that it lies in the middle of linear dynamic range of the assay. So, for example: If I consider (for IL1beta) 31.2pg/ml-2000pg/ml as the Assay Range, the middle of the assay range would be around 1000pg/ml. But this range refers to IL1beta, not total proteins. Measuring plasma total proteins would result in much higher protein levels when compared to IL1beta levels. Could you please explain me how should I proceed with these dilutions/protein quantification in more detail? Is there a standardized total protein amount of samples that should be pipetted per well, so that it lies within the assay range? I only found this general tip: "Serum, plasma, cell and tissue extracts are typically diluted by 50% with binding buffer". (ELISA sample preparation guide).

Verified Customer

Verified customer

Asked: 2019-06-28

Answer

Please suggest the customer use the following receommeded dilution ratio for rat serum samples. EK0418 1:1 EK0514 1:100 EK0393 1:1 It is useless and unnecessary to quantify the amount of total protein. Add 100ul of diluted sample into each well.

Boster Scientific Support

Answered: 2019-06-28

Question

Q: how are cell lysates prepared for use in Picokine® ELISA kits?

Verified Customer

Verified customer

Asked: 2019-05-24

Answer

A: for those Picokine® ELISAs where cell or tissue lysate is a validated sample type, sample preparation instructions for lysate are included in the product insert. Components in lysate and lysis buffer can impact immunoreactivity, so if lysate is not a validated sample type, care must be taken in sample preparation and validation.

Boster Scientific Support

Answered: 2019-05-24

Question

Q: Are Boster Bio recombinant proteins and antibodies sterile?

Z. Chen

Verified customer

Asked: 2018-09-10

Answer

A: although the vials are bottled using aseptic techniques, heat-treated vials, and sterile stock solutions, they are not considered or guaranteed to be sterile. If sterile material is needed for an experiment, the material can be filtered through a 0.2 micron filter designed for use with biological fluids.

Boster Scientific Support

Answered: 2018-09-10

Question

Q: we need your suggestion regarding the dilution ratio of serum samples for detection of IL-10 in Rat plasma? I am trying to measure a few parameters and it requires 100ul of diluted samples for each well. We have limited sample quantitys so we like to dilute as much as possible.

Verified Customer

Verified customer

Asked: 2018-04-13

Answer

A: unable to understand the physiological or pathological context of your samples we cannot recommend a dilution ratio without performing a pilot test with your samples. Here is how you can perform a pilot study on your own: perform a serial dilution of your samples on the IL-10 ELISA kit to make sure you have a linear ascending curve followed by a plateau, which signifies the samples saturating the detection limit of the kit. Then you can pick the dilution ratios from samples in the linear part of the curve as your experimental dilution ratio.
If you are interested in using our ELISA service, you can also send us your sample and we will take care of everything for you. You can check our service details here: bosterbio.com/services/assay-services/ELISA-testing-service
Since you mentioned you have limited samples, our cost effective multiplex ELISA service would fit perfectly for your needs, where we can generate dozens of data points using as little as 25ul sample volume. Information on this service is also in the above link.

Boster Scientific Support

Answered: 2018-04-13

Question

Q: What is the optimal O.D. value for IL-10 ELISA kit? I performed your IL-10 ELISA on serum samples. For my positive control, I received an O.D. value of 0.826, while my negative control received a value of 0.136. I obtained both of these controls from the ELISA kit, where your kit's typical data shows O.D. values much higher than my positive control and your background is lower. My samples O.D. values are around 0.225 and the highest is only 0.357. can I consider these samples contain IL-10 even though the O.D. values are not very high?

M. Parker

Verified customer

Asked: 2014-09-05

Answer

A: The absolute O.D. values may change according to incubation time. The more you incubate the higher the O.D. values are going to be. what you should focus on is whether your sample O.D. values are statistically significantly higher than your blank values. in the above example, you could extend your development time in the substrate incubation step to obtain higher O.D. values, as long as your negative controls' O.D. values are not increasing faster in proportion to your positive controls. normally, a sample with O.D. value 2 standard deviations higher than your negative controls can be considered positive. We calculate the sensitivity of this ELISA kit by converting cutoff O.D. value, calculated as the average of 20 negative controls plus 2 standard deviations of the 20 negative controls, into a concentration. in other words, when we claim this IL-10 ELISA kit to have sensitivity of 4pg/ml, that means the minimum amount of IL-10 that can be declared/interpreted as positive by the above standard is 4pg/ml.

Boster Scientific Support

Answered: 2014-09-05

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