Introduction
PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. Cyclophilin-A is a cyclosporin binding-protein and may play a role in cyclosporin A-mediated immunosuppression. Cyclophilin-A can also interact with several HIV proteins, including p55 gag, Vpr, and capsid protein, and has been shown to be necessary for the formation of infectious HIV virions. Multiple pseudogenes that map to different chromosomes have been reported.
Overview
| Product Name |
Rat KIM1 ELISA Kit PicoKine™
See all TIM-1/KIM-1/HAVCR primary antibodies, ELISA kits and proteins |
| SKU/Catalog Number |
EK0882 |
| Storage & Handling |
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles(Shipped with wet ice.) |
| Description |
Detect Rat Kim 1/HAVCR1 with <2pg/ml sensitivity. Format: 96-well plate with removable strips. Compatible samples: cell culture supernates, serum, plasma(heparin, EDTA) and urine. This is a TMB colorimetric sandwich ELISA kit with short assay time and fast experiment set up. Kim 1/HAVCR1 tissue specificity: Expressed at a low level in normal kidney but are increased dramatically in postischemic kidney. Expressed in proliferating bromodeoxyuridine-positive and dedifferentiated vimentin-positive epithelial cells in regenerating proximal tubules. . |
| Cite This Product |
Rat KIM1 ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0882)
|
Product Specs
| Reactivity/Species |
Rat |
| Applications |
ELISA
*Our Boster Guarantee covers the use of this product in the above
tested applications.
**For positive and negative control design, consult "Tissue specificity" under
"Protein Target Info" tab. |
| Sample Type |
cell culture supernates, serum, plasma(heparin, EDTA) and urine. |
| Sensitivity |
<2pg/ml |
| Assay Range |
31.2pg/ml-2000pg/ml |
| Immunogen/Standard |
Expression system for standard: NSO; Immunogen sequence: S18-V238 |
| Antibody Clonalities |
Capture antibody|Detection antibody
monoclonal antibody from mouse|polyclonal antibody from goat |
| Cross Reactivity |
There is no detectable cross-reactivity with other relevant proteins. |
| Pack Size |
96wells/kit, with removable strips. |
Gene/Protein Basic Information For Havcr1 (Source: Uniprot.org, NCBI)
| Uniprot Id | O54947 |
|---|
| NCBI Gene Id | 286934 |
|---|
| Species Of This Entry | Rat |
|---|
| Gene Name | Havcr1 |
|---|
| Protein Name | Hepatitis A virus cellular receptor 1 homolog |
|---|
| Superfamily | immunoglobulin superfamily |
|---|
| Alternative Names | TIM-1/KIM-1/HAVCR|CD365; HAVCR1; HAVCR-1; HAVCRT cell immunoglobin domain and mucin domain protein 1; hepatitis A virus cellular receptor 1; Kidney injury molecule 1; KIM1; KIM-1; T-cell immunoglobulin and mucin domain-containing protein 1; TIM1; TIM-1; TIM-1TIM; TIM1TIMD-1; TIMD1T-cell membrane protein 1 |
|---|
| Molecular Weight | 33964 |
|---|
| Post Tranlational Modifications | Phosphorylation; Glycosylation; Cleavage; Deacetylation; Acetylation; Methylation |
|---|
| Gene Location | 10q21, on Chromasome 10, gene sequence: |
|---|
*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
Ontology For Havcr1 (Source: Uniprot.org, NCBI)
| Protein Function | May play a role in T-helper cell development and the regulation of asthma and allergic diseases. Receptor for TIMD4. May play a role in kidney injury and repair (By similarity). . |
|---|
| Tissue Specificity | Expressed at a low level in normal kidney but are increased dramatically in postischemic kidney. Expressed in proliferating bromodeoxyuridine-positive and dedifferentiated vimentin-positive epithelial cells in regenerating proximal tubules. |
|---|
| Related Diseases | Injury To Kidney; Acute Kidney Injury; Kidney Diseases; Hepatitis; Hepatitis A; Asthma; Inflammation; Kidney Failure, Chronic; Kidney Failure; Chronic Kidney Disease; Proteinuria Of Undiagnosed Cause; Autoimmune Reaction; Diabetic Nephropathy; Allergy; Ischemia; Diabetes Mellitus; Autoimmune Diseases; Fibrosis; Malignant Neoplasms |
|---|
| Related Pathways | Glomerular Filtration; Excretion; Pathogenesis; Cell Activation; Immune Response; Cytokine Production; T Cell Activation; Cell Proliferation; Cell Differentiation; T Cell Proliferation; Phagocytosis; Cell Death; Secretion; T Cell Differentiation; Cell Adhesion; Lymphocyte Proliferation; Regeneration; Dedifferentiation; Lymphocyte Activation; Programmed Cell Death |
|---|
| Background | KIM1(TIM-1), also known as Hepatitis A virus cellular receptor 1, is a protein that in Rat is encoded by the HAVCR1 gene. Infection of canine osteogenic sarcoma cells expressing HAVCR1 with HAV led to conclude that the protein is indeed a receptor for the virus. Immunofluorescence microscopy demonstrated internalization of HAV by dog cells expressing HAVCR1. Using a monoclonal antibody to rat Tim1, Tim1 was expressed after activation of naive T cells and on T cells differentiated in Th2-polarizing conditions. By homology of synteny with the rat Tim1 gene and database analysis, was mapped the HAVCR1 gene to 5q33.2. |
|---|
| Scientific References | PMID: 9461608 by Ichimura T., et al. Kidney injury molecule-1 (KIM-1), a putative epithelial cell adhesion molecule containing a novel immunoglobulin domain, is up-regulated in renal cells after injury. |
|---|
Kit Components And Assay QC Data Details Of Rat KIM1 ELISA Kit PicoKine™
*The quality control (QC) data in this section is obtained from Boster's internal QC results and is for reference only. It may differ from the users' lab test results. The users can expect to generate data with similar linearity and quality demonstrated in the typical data but may not achieve exactly the same O.D. values.
| Catalog number | Description | Quantity |
| EK0882-CAP | 96-well plate precoated with anti-Rat TIM-1/KIM-1/HAVCR antibody | 1 |
| EK0882-ST | lyophilized recombinant Rat TIM-1/KIM-1/HAVCR standard | 10ng/tube |
| EK0882-DA | biotinylated anti-Rat TIM-1/KIM-1/HAVCR antibody | 130ul |
| AR1103 | Avidin-Biotin-Peroxidase Complex(ABC) | 130ul |
| AR1106-1 | sample diluent buffer | 30ml |
| AR1106-2 | antibody diluent buffer | 12ml |
| AR1106-3 | ABC diluent buffer | 12ml |
| AR1104 | TMB color developing agent | 10ml |
| AR1105 | TMB stop solution | 10ml |
| PLA-SEA | Adhesive cover | 4 |
- Microplate reader in standard size.
- Automated plate washer.
- Adjustable pipettes and pipette tips. Multichannel pipettes are recommended in the condition of large amount of samples in the detection.
- Clean tubes and Eppendorf tubes.
- Washing buffer (neutral PBS or TBS).
- Preparation of 0.01M TBS: Add 1.2g Tris, 8.5g NaCl; 450μl of purified acetic acid or 700μl of concentrated hydrochloric acid to 1000ml H2
- Preparation of 0.01 M PBS: Add 8.5g sodium chloride, 1.4g Na O and adjust pH to 7.2-7.
This data is generated from a recent batch of EK0882 Rat KIM1 ELISA Kit PicoKine™. It is not necessarily the same data set used to generate the standard curve shown in the image of this product. TMB reaction incubate at 37°C for 15-25min
| Concentration(pg/ml) | 0 | 31.2 | 62.5 | 125 | 250 | 500 | 1000 | 2000 |
| O.D. | 0.029 | 0.115 | 0.171 | 0.282 | 0.560 | 0.900 | 1.538 | 2.256 |
We measured random samples of Rat KIM1 ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
| Intra-Assay Precision | Inter-Assay Precision |
|---|
| Sample | 1 | 2 | 3 | 1 | 2 | 3 |
| n | 16 | 16 | 16 | 24 | 24 | 24 |
| Mean(pg/ml) | 87 | 272 | 938 | 86 | 293 | 1026 |
| Standard deviation | 3.82 | 20.67 | 59.09 | 3.95 | 23.14 | 84.13 |
| CV(%) | 4.4% | 7.6% | 7.9% | 4.6% | 7.9% | 8.2% |
We measured the performance consistency of Rat KIM1 ELISA Kit PicoKine™ to ensure the reproducibility of the assays. Three samples with differing target protein concentrations were assayed using four different lots.
Reproducibility
To assay reproducibility, three samples with differing target protein concentrations were assayed using four different lots.
| Lots | Lot1 (pg/ml) | Lot2 (pg/ml) | Lot3 (pg/ml) | Lot4 (pg/ml) | Mean (pg/ml) | Standard Deviation | CV (%) |
|---|
| Sample 1 | 87 | 94 | 102 | 93 | 94 | 5.33 | 5.6% |
| Sample 2 | 272 | 238 | 277 | 253 | 260 | 15.54 | 5.9% |
| Sample 3 | 938 | 849 | 928 | 895 | 902 | 34.74 | 3.8% |
*number of samples for each test n=16.
Specific Protocols
Boster provides comprehensive technical information for WB, IHC/IF/ICC, Flow Cytometry sample preparation protocols, assay protocols, troubleshooting tips and assay optimization tips.
See all TIM-1/KIM-1/HAVCR primary antibodies,
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Total number of citations: 6
| Baisong Zhao, Yongying Pan, Zixin Wang, Haiping Xu and Xingrong Song. Yonsei Med J. 2017 Jan;58(1):131-138. Hyperbaric Oxygen Pretreatment Improves Cognition and Reduces Hippocampal Damage Via p38 Mitogen-Activated Protein Kinase in a Rat Model |
PubMed ID - |
| Involvement of the NF-?B signaling pathway in the renoprotective effects of isorhamnetin in a type 2 diabetic rat model |
PubMed ID 27123259 |
| Glycyrrhetinic acid might increase the nephrotoxicity of bakuchiol by inhibiting cytochrome P450 isoenzymes |
PubMed ID 27904813 |
| Exogenously administered adenosine attenuates renal damage in streptozotocin-induced diabetic rats |
PubMed ID 27418253 |
| Exogenously administered adenosine attenuates renal damage in streptozotocin-induced diabetic rats |
PubMed ID 27418253 |
| Protective effect of Salvianolic acid A on ischaemia-reperfusion acute kidney injury in rats through protecting against peritubular capillary endothelium damages |
PubMed ID 29071768 |
