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Product Info Summary
|Size:||96 wells/kit, with removable strips.|
|Sample Types:||cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).|
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Rat Laminin ELISA Kit PicoKine™
96 wells/kit, with removable strips.
*Question: How many samples can I assay/run in this kit?
Rat Laminin ELISA Kit PicoKine™ (96 Tests). Quantitate Rat LAMA1 in cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA). Sensitivity: 10pg/ml.
Storage & Handling
Store at 4°C for 6 months, at -20°C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)
Cite This Product
Rat Laminin ELISA Kit PicoKine™ (Boster Biological Technology, Pleasanton CA, USA, Catalog # EK0435)
Clonality of Antibodies
See Datasheet for details
Expression system for standard: from rat sarcoma basement membrane
156 pg/ml - 10,000 pg/ml
There is no detectable cross-reactivity with other relevant proteins.
EK0435 is reactive to LAMA1 in Rat samples
Validated Sample Types
cell culture supernatants, cell lysates, serum and plasma (heparin, EDTA).
EK0435 is guaranteed for ELISA in Rat by Boster Guarantee
See how Boster Bio validate our ELISA kits: ELISA Validation Information
|EK0435-CAP||Anti-Rat LAMA1 Pre-coated 96-well strip microplate||1|
|EK0435-ST||Rat LAMA1 Standard||2 vials, 10 ng/tube|
|EK0435-DA||Rat LAMA1 Biotinylated antibody (100x)||100ul|
|AR1103||Avidin-Biotin-Peroxidase Complex (100x)||100ul|
|AR1104||Color Developing Reagent (TMB)||10ml|
|AR1106-5||Wash Buffer (25x)||20ml|
|PLA-SEA||Adhesive plate sealers||4|
*The kit components are not available for individual purchase.
Materials Required But Not Included In Kit
- Microplate Reader capable of reading absorbance at 450nm.
- Automated plate washer (optional).
- Pipettes and pipette tips capable of precisely dispensing 0.5 µl through 1 ml volumes of aqueous solutions.
- Multichannel pipettes are recommended for large amount of samples.
- Deionized or distilled water.
- 500ml graduated cylinders.
- Test tubes for dilution.
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Validation Standard Curve O.D. At 450nm
Data Example Images
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Rat LAMA1 PicoKine ELISA Kit standard curve
Intra Assay Consistency & Inter Assay Consistency
We measured random samples of Rat Laminin ELISA Kit PicoKine™ within the same batch/lot to ensure the consistency of the kits' performances. ELISA intra assay consistency is measured using wells from the same plate/assay kit. ELISA inter assay consistency is measured using wells from different plates from the same batch production/lot.
|Intra-Assay Precision||Inter-Assay Precision|
We ensure reproducibility by testing three samples with differing concentrations of Laminin S/Laminin beta 2 in ELISA kits from four different production batches/lots.
|Lots||Lot 1 (pg/ml)||Lot 2 (pg/ml)||Lot 3 (pg/ml)||Lot 4 (pg/ml)||Mean (pg/ml)||Standard Deviation||CV (%)|
Gene/Protein Information For LAMA1 (Source: Uniprot.Org, NCBI)
Laminin subunit beta-2
LAMB2; Laminin B1s chain; Laminin beta 2; Laminin S; laminin subunit beta-2; laminin, beta 2 (laminin S); Laminin-11 subunit beta; Laminin-14 subunit beta; Laminin-15 subunit beta; Laminin-3 subunit beta; Laminin-4 subunit beta; Laminin-7 subunit beta; LAMSLaminin-9 subunit beta; S-LAM beta; S-laminin subunit beta Lamb2|SLAM|laminin subunit beta 2|laminin subunit beta-2|S-LAM beta|S-laminin subunit beta|laminin chain B3|laminin chain beta 2|laminin, beta 2|laminin-11 subunit beta|laminin-14 subunit beta|laminin-15 subunit beta|laminin-3 subunit beta|laminin-4 subunit beta|laminin-7 subunit beta|laminin-9 subunit beta*if product is indicated to react with multiple species, protein info is based on the gene entry specified above in "species".
For more info on LAMA1, check out the LAMA1 Infographic
We have 30,000+ of these available, one for each gene! check them out.
In this infographic you will see the following information for LAMA1: database IDs, super-family, protein function, synonyms, molecular weight, chromosomal locations, tissues of expression, subcellular locations, post translational modifications, and related diseases, research areas & pathways. If you want to see more information included, or would like to contribute to it and be acknowledged, please contact us [email protected]
EK0435 has been cited in 1 publications:
*The publications in this section are manually curated by our staff scientists. They may differ from Bioz's machine gathered results. Both are accurate. If you find a publication citing this product but is missing from this list, please let us know we will issue you a thank-you coupon.
Lin Cy, Li Lt, Su Wt. Mater Sci Eng C Mater Biol Appl. 2014 Sep;42:474-8. Doi: 10.1016/J.Msec.2014.05.063. Epub 2014 Jun 6. Three Dimensional Chitosan Scaffolds Influence The Extra Cellular Matrix Expression In Schwann Cells.
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